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Molecular Biology Final Topics > Laboratory Questions > Flashcards

Laboratory Questions Flashcards

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1
Q

The noncoding region of gene, locked between exons, that will not be translated into proteins.

A

Introns

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2
Q

How do helicases untangle DNA?

A

By cutting and ligating one or both strands of the double helix

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3
Q

Which DNA polymerase is primarily involved in DNA repair?

A

Pol B

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4
Q

Which enzyme recognizes specific base sequences in DNA and breaks or restricts the DNA polymer at the sugar-phosphate backbone?

A

Endonucleases

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5
Q

An example of simple proteins.

A

Histones

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6
Q

A derived protein.

A

Fibrin and Proteases

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7
Q

Defect in chromosome 15.

A

Prader-Willi syndrome and Angelmann syndrome

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8
Q

Pipettes are calibrated every?

A

6 months

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9
Q

Recommended pipette for water.

A

Air-displacement

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10
Q

Recommended pipette for ammonium hydroxide.

A

Positive-displacement

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11
Q

Recommended pipette for hydrochloric acid.

A

Positive-displacement

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12
Q

Samples are spotted in the center of the paper, high voltage is applied, and the spots migrate according to their charges.

A

Paper

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13
Q

Separates amphoteric molecules (have both acidic and basic/alkaline properties) according to their charge as defined by the pKA values of proton-accepting sites within a molecule.

A

Tubes (isoelectric focusing)

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14
Q

Separation technique of molecules and ions performed in a narrow capillary tube structure using electric current.

A

Capillaries

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15
Q

Extracted from seaweeds.

A

Agarose gel

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16
Q

Application of heat to separate dsDNA (template) into two single strands.

A

Denaturation

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17
Q

Binding of primers to the ssDNA template by hybridization.

A

Annealing

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18
Q

DNA polymerase obtained from Thermus aquaticus.

A

Taq polymerase

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19
Q

DNA polymerase obtained from Thermococcus litoralis.

A

Vent polymerase

20
Q

Number of cycles is usually?

A

30-50 cycles

21
Q

Primers can be multiple (more than a pair)

A

Multiplex PCR

22
Q

Uses 2 pairs of primers for a single target. Second pair will bind slightly inside the binding site of first pair.

A

Nested PCR

23
Q

AKA quantitative PCR (qPCR).

A

Real-time PCR

24
Q

It reduces premature DNA polymerase activity during the initial stages of the reaction setup.

A

Hot start PCR

25
Q

Causative agent of whooping cough?

A

Bordetella pertussis

26
Q

The causative agent of the 3rd most common cause of community-acquired pneumonias.

A

Legionella pneumophilia

27
Q

Causative agent of Guilian-Barre syndrome.

A

Chlamydophilia pneumoniae

28
Q

Also known as the Acid-fast bacilli.

A

Mycobacterium tuberculosis

29
Q

Specimen choice of MTB

A

Sputum and Gastric Aspirators

30
Q

Also known as the walking pneumonia.

A

Mycoplasma pneumoniae

31
Q

Causative agent of “flow seed”.

A

Neisseria gonorrhoeae

32
Q

Recommended microscope for Treponema pallidium.

A

Dark field microscope

33
Q

Causative agent of genital ulcer.

A

Haemophilus ducreyi

34
Q

Specimen of choice for Haemophilus ducreyi

A

Ulcer

35
Q

Antibody involve in allergies.

A

IgE

36
Q

Standard screening for HIV.

A

ELISA

37
Q

Fluorescent Glycoprotein Method

A

Western blot

38
Q

Type of HIV that is more pathogenic.

A

HIV-1

39
Q

Type of herpes that causes genital herpes.

A

HSV-2

40
Q

Virus associated with Hodgkin lymphoma.

A

EBV

41
Q

Causative agent of chicken pox.

A

Varicella Zoster

42
Q

Diagnostic Method for Varicella Zoster.

A

Gel electrophoresis and PCR with hybrdization

43
Q

Gold standard method for detection of SARS-CoV 2.

A

RT-PCR

44
Q

Conventional method for detect parasite.

A

Morphology by microscopy

45
Q

How many percent of Chocolate in Chocolate agar?

A

0 or None

46
Q

Recommended swab for Bordetella pertussis.

A

Calcium alginate and Darcon polyester with flexible wire shaft

47
Q

How many swabs are collected for Bordetella pertussis?

A

2

Molecular Biology Final Topics (2 decks)

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