Laboratory Questions Flashcards
The noncoding region of gene, locked between exons, that will not be translated into proteins.
Introns
How do helicases untangle DNA?
By cutting and ligating one or both strands of the double helix
Which DNA polymerase is primarily involved in DNA repair?
Pol B
Which enzyme recognizes specific base sequences in DNA and breaks or restricts the DNA polymer at the sugar-phosphate backbone?
Endonucleases
An example of simple proteins.
Histones
A derived protein.
Fibrin and Proteases
Defect in chromosome 15.
Prader-Willi syndrome and Angelmann syndrome
Pipettes are calibrated every?
6 months
Recommended pipette for water.
Air-displacement
Recommended pipette for ammonium hydroxide.
Positive-displacement
Recommended pipette for hydrochloric acid.
Positive-displacement
Samples are spotted in the center of the paper, high voltage is applied, and the spots migrate according to their charges.
Paper
Separates amphoteric molecules (have both acidic and basic/alkaline properties) according to their charge as defined by the pKA values of proton-accepting sites within a molecule.
Tubes (isoelectric focusing)
Separation technique of molecules and ions performed in a narrow capillary tube structure using electric current.
Capillaries
Extracted from seaweeds.
Agarose gel
Application of heat to separate dsDNA (template) into two single strands.
Denaturation
Binding of primers to the ssDNA template by hybridization.
Annealing
DNA polymerase obtained from Thermus aquaticus.
Taq polymerase
DNA polymerase obtained from Thermococcus litoralis.
Vent polymerase
Number of cycles is usually?
30-50 cycles
Primers can be multiple (more than a pair)
Multiplex PCR
Uses 2 pairs of primers for a single target. Second pair will bind slightly inside the binding site of first pair.
Nested PCR
AKA quantitative PCR (qPCR).
Real-time PCR
It reduces premature DNA polymerase activity during the initial stages of the reaction setup.
Hot start PCR
