LABORATORY MANUAL IN HEMATOLOGY Flashcards
What are the methods of obtaining blood specimens?
VAS
- Skin puncture
- venipuncture
- arterial puncture
This is commonly obtained using skin puncture
Capillary puncture
Capillary puncture is commonly obtained using what method?
Skin puncture
Venous blood is obtained by what method?
Venipuncture
This is obtained by arterial puncture
Arterial blood
This method is not included in current medical technology practice in the Philippines
Arterial blood extraction
This is obtained from the tip of the 3rd or 4th finger or the earlobe in adults
Peripheral blood
Peripheral blood is obtained from what body part/s in adults
from the tip of the 3rd or 4th finger or the earlobe in adults
In obtaining peripheral blood, wipe the area with a cotton ball soaked in what?
70% isopropyl alcohol
The puncture depth in obtaining a peripheral blood sample must be how deep?
2-3 mm in depth
Difficulty in obtaining blood can be relieved how?
By putting warm compress on the site
This is essential for tests that require anticoagulation (except for microtechniques) and when a large quantity of blood is needed
Venipuncture
Why is venipuncture the most convenient method of blood collection?
Because it reduces the number and variety of apparatus required
Venous blood is usually obtained from what veins?
antecubital veins
What are these antecubital veins?
- median cubital
- cephalic
- basilic
Venipuncture can be performed using what (2) methods?
- syringe method
- evacuated tube method
The tourniquet must be how many inches above the puncture site?
3-4 inches
In what manner must the tourniquet be tied?
in a half-knot manner
In what manner must the tourniquet be tied?
in a half-knot manner
What is the recommended needle gauge to be used for syringe method?
20-21 gauge needle
At what angle must the syringe be inserted?
15 degree angle
in syringe method, how deep must the syringe go?
0.5 - 1 cm
What is the additive in plain red tube?
none
At how many minutes will the blood clot in a plain red tube?
clot formation in 30mins
Plain red tube is used for what?
- blood chemistry
- serology
- blood banking
Which lavendar tube is contained in a glass?
Liquid K2EDTA
Which lavendar tube is contained in plastic?
spray-dried k2 edta
What are the additives in a lavendar tube?
- liquid k2 edta
- spray-dried k2 edta
What is the function of the additives present in the lavendar tube?
binds calcium to prevent clotting
How many times must the lavendar tube be inverted?
8 times
Lavendar tube is used for blood cell counting
True or False
true
What is the additive present in light blue (glass) tube?
3.2 sodium citrate
What is the additive present in light blue (plastic) tube?
3.8 sodium citrate
