LABORATORY MANAGEMENT Flashcards
5 HIERARCHY OF CONTROLS (from most to least effective)
ELIMINATION
SUBSTITUTION
ENGINEERING CONTROLS
ADMINISTRATIVE CONTROLS
PPE
Removes hazard at the source
Preferred solution to protect workers
because no exposure can occur
Elimination
using a safer alternative to the source of
the hazard
effective substitutes reduce the potential
for harmful effects and do not create new
risks
SUBSTITUTION
reduce or prevent hazards from coming
into contact with workers.
Can include modifying equipment or the
workspace, using protective barriers,
ventilation, and more.
Can cost more than administrative
controls or PPE. However, long-term
operating costs tend to be lower,
especially when protecting multiple
workers.
Engineering
Controls
Establish work practices that reduce the
duration, frequency, or intensity of
exposure to hazards.
Administrative
Controls
Equipment worn to minimize exposure to
hazards
PPE
Might seem to be less expensive than
other controls, but can be costly over
time.
PPE
Biological hazards are disease producing agents (pathogens) that can be transmitted to individuals through various routes of exposure (modes of transmission). Exposure to these hazards may result in acute or chronic health conditions.
BIOHAZARDS
5 COMMON MODES OF TRANSMISSION
- BLOOD AND BODY FLUIDS
- FECAL-ORAL
- CONTACT
- SMALL PARTICLE AEROSOL (AIRBORNE)
- More than one mode: contact,
and/or droplet spray
EXAMPLES OF PATHOGENS: BLOOD AND BODY FLUIDS
Hepatitis B Virus (HBV)
Hepatitis C Virus (HCV)
Human Immunodeficiency Virus
(HIV)
EXAMPLES OF PATHOGENS: FECAL-ORAL
Hepatitis A Virus (HAV)
Hepatitis E Virus (HEV)
MODE OF TRANSMISSION:
Herpes Simplex Viruses (HSV)
Human Papilloma Virus (HPV)
Cytomegalovirus (CMV)
Vancomycin Resistant
Enterococcus (VRE)
Methicillin Resistant
Staphylococcus Aureus (MRSA)
Clostridium difficile (C. difficile)
CONTACT
EXAMPLES OF PATHOGENS: AIRBORNE
Mycobacterium Tuberculosis
More than one mode: contact,
and/or droplet spray
Severe Acute Respiratory
Syndrome Coronavirus 2 (SARSCoV-2)
Herpes Zoster Virus (HZV)
Influenza virus (Flu)
Measles (Rubeola virus)
CHAIN OF INFECTION
MICROORGANISM
RESERVOIR/SOURCE
PORT OF EXIT
MOT
PORT OF ENTRY
SUSCEPTIBLE HOST
bacteria, virus, fungus, parasite
microorganisms
habitat for organisms to grow: human, animal, air, food, soil, water, equipment
reservoir/source
SECRETION (SALIVA)
EXCRETION (URINE, FECES)
PORT OF EXIT
CONTACT, DROPLET, AIRBORNE, VECTOR, FORMITES
MODE OF TRANSPORT
MUCOSA LINING, OPEN WOUND, ORAL, RESPIRATORY TRACT, URINARY TRACT
PORT OF ENTRY
VERY YOUNG & ELDERLY, WITH CHRONIC DISEASE, MALNOURISHED, IMMUNOCOMPROMISED
SUSCEPTIBLE HOST
Agents that have no known potential for infecting healthy people
BSL1
Microorganisms associated with human diseases that are rarely serious and for which preventive or therapeutic interventions are often available.
BSL2
Material that may contain viruses not normally encountered in a clinical laboratory and mycobacteria
BSL 3 WITH RISK BASED
ENHANCEMENTS
dangerous and exotic agents that pose a higher risk of aerosol transmitted laboratory infections and life-threatening disease for which effective treatments are limited.
BSL4
- Controlled access
- Hand washing sink
- Sharp hazards warning policy
- Personal protective equipment
- Laboratory bench
- Autoclave
BSL1
- Controlled access
- Hand washing sink
- Sharp hazards warning policy
- Physical containment device
- Personal protective equipment
- Laboratory bench
AUTOCLAVE
BSL2
*Air tight when disinfecting
* Self-closing, double-door access
* Controlled access
* Personal shower out (risk-based
enhancement)
* Sharp hazards warning policy
* Hand washing sink
* Sealed penetrations
* Physical containment device
* Powered air purifying respirator
(risk-based enhancement)
* Laboratory bench
* Autoclave
* Exhaust HEPA filter (risk-based
enhancement)
* Effluent decontamination system
(risk-based enhancement)
BSL 3 WITH RISK BASED
ENHANCEMENTS
- Air tight
- Self-closing, double-door access
- Controlled access
- Sharp hazards warning policy
- Hand washing sink
Sealed penetrations
Physical containment device
Positive pressure protective suit
Laboratory bench
Autoclave
Chemical shower out
Personal shower out
Supply and exhaust HEPA filter
Effluent decontamination system
BSL4
Most important means of preventing the spread of infection
HAND WASHING
If hands are visibly soiled, wash hands with soap and water for ___
mins
1-2 MINS
If hands are not visibly soiled, wash hands for at least ___. or use
____ hand cleaners
30 secs
alcohol based
*CDC Guidelines: During hand washing, thoroughly clean between your fingers and under your fingernails for at least _____. And rinse your hands in a _____ position to prevent recontamination.
20 secs
downward
Worn to protect the clothing and skin of
health
Protect workers from contamination
Should be put on first
lab gown
Worn to protect against splashes and
inhalation of droplets containing
pathogens from infective patients.
mask, googles, face shields
Worn to protect healthcare workers from contamination by patient body substances
gloves
DONNING
1.Gown should be put on first
2.Mask should cover both nose
and mouth
3.Gloves should be pulled over the
gown cuff
DOFFING
1.Gloves are removed first.
2.Gown should be pulled from the
shoulders towards the hands
3. Mask should be removed
Decontamination of Body Fluid Spills
✓ _______
✓ Recommended contact time is _____
5.25% NaCL or 10% chlorine bleach (1:10 dilution)
15 minutes
enclosed workspaces with a ventilated hood that is designed to contain pathogenic microorganisms during microbiological processes.
Biosafety Cabinets
Class I and II Biosafety cabinets are used for Biosafety levels __ and __
I and II
Class III BSCs are most suitable for work with hazardous agents that require Biosafety Level __OR __
3 OR 4
the most basic biosafety cabinet that provides
protection to the environment and the laboratory personnel.
CLASS 1
It doesn’t provide protection to the product as the unsterilized room air is drawn over the work surface.
CLASS 1
cabinets protect the operator and the environment from the aerosol but not the sample.
CLASS 1
provide both kinds of protection (of the samples and the environment) since makeup air is also HEPA- filtered.
CLASS II
5 TYPES OF CLASS II BIOSAFETY CABINETS
TYPE A1
TYPE A2
TYPE B1
TYPE B2
TYPE C1
The type A1 cabinets have a minimum inflow
velocity of ____
75ft/min
This type of cabinet is not as widely used as it is not safe to work with hazardous chemical substances.
CLASS II TYPE A1
The type A2 cabinets have a minimum inflow
velocity of
100 ft/min.
Because of the chances of the release of
hazardous chemicals into the environment, _____ cabinets are also not extensively used.
CLASS II TYPE A2
These cabinets have a dedicated duct system
which allows the release of the contaminated air
out of the facility.
CLASS II TYPE B1
In the case of Type B1 cabinets, ___ of the air is recirculated, whereas the remaining ___ is exhausted out of the facility
40%
60%
All of the contaminated airflow (100%) in these cabinets is externally exhausted which means the air drawn into the cabinet is 100% exhausted into the atmosphere.
Because none of the air is recirculated, these
cabinets are the best to be used for tasks involving the release of chemical vapors.
CLASS II TYPE B2
expensive, and their use is limited to toxicology laboratories where protection against hazardous chemicals is imperative.
CLASS II TYPE B2
Similar to Type B cabinets in their working mechanism, but these are designed to reduce operating costs add flexibility to the laboratories.
the cabinets provide protection to the
environment, the operator, and the workstation or
the biological material.
CLASS II TYPE C1