Laboratory: Lipids and Lipoproteins Flashcards

1
Q

Combining of complex lipids is called

A

Esterification

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2
Q

Plasma is 93% water but can be mixed with lipids due to
lipoproteins via coating. True or False?

A

True

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3
Q

Largest lipoprotein but least dense

A

Chylomicron

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4
Q

These two are the transporters of triglycerides

A

Chylomicrons
VLDL

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5
Q

These two are the transporters of cholesterol

A

LDL
HDL

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6
Q

Chylus specimen indicates post-meal with the presence of __?

A

White creamy float

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7
Q

What causes the turbidity of specimen?

A

VLDL

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8
Q

VLDL increase is from protein increase. True or False?

A

False, it is from carbohydrate, saturated fatty acids and trans fatty acids (dietary absorption)

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9
Q

Agent that increases risk of
atherosclerosis

A

Proatherogenic

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10
Q

It carries cholesterol from the liver to the tissues

A

LDL

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11
Q

Endogenous or stored TGs are carried by ____ from
liver to tissues

A

VLDL

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12
Q

Prevents occurrence of athersclerosis

A

Antiatherogenic

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13
Q

Higher LDL can mean in lower risk of cardiovascular
disease. True or False?

A

False, it is higher HDL that indicates lower risk of CVD

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14
Q

Structure of HDL that does not carry any lipids

A

Discoidal or nascent

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15
Q

HDL that carries lipids are most commonly seen as what shape or structure?

A

Spherical

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16
Q

Delay or error in conversion of VLDL to LDL

A

Type III hyperlipoproteinimia

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17
Q

Transient products of VLDL lipolysis

A

IDL

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18
Q

Similar structure with VLDL and LDL minus Apo B-100, replaced by albumin

A

Lipoprotein X

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19
Q

Elevated levels of LP X can result to kidney failure. True or False?

A

True

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20
Q

Determination of of size of lipoprotein is based on _?

A

Lipid content

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21
Q

Density of lipoprotein is based on _?

A

Protein content

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22
Q

VLDL decreases when adipocyte releases free fatty
acids. True or False?

A

False, VLDL increases

23
Q

Apo A is found in _

A

HDL

24
Q

Apo A-IV is found in _ and _

A

HDL
Chylomicrons

25
Q

Apo B-100 is found in _

A

LDL

26
Q

Apo B-48 is found in _

A

Chylomicrons

27
Q

Apo C is found in _, _ and _

A

VLDL, HDL and LDL

28
Q

Apo D is found in _

A

HDL

29
Q

Apo E is found in _, _, _ and _

A

VLDL
IDL
Chylomicrons
HDL

30
Q

Total Cholesterol is obtained via addition of?

A

HDL, LDL and VLDL

31
Q

SI method for computation of VLDL

A

Plasma TG / 2.175

32
Q

Conventional method for computation of VLDL

A

Plasma TG / 5.0

33
Q

Prolonged tourniquet application causes ___

A

False elevation of lipid content in blood

34
Q

Lipoproteins that requires fasting

A

TG
LDL

35
Q

Lipoproteins that does not require fasting

A

TC
HDL

36
Q

Male have lower normal range than female. True or False?

A

False, female have lower normal range

37
Q

Conversion factor of cholesterol

A

0.026

38
Q

Conversion factor of TG

A

0.0113

39
Q

Bloor’s reagent extraction and KOH saponification is used in what cholesterol method?

A

Abell-Kendal Method

40
Q

Extract with Xeolite and use of acetic anhydride and sulfuric acid is what cholesterol method?

A

Liebermann-Buchard

41
Q

Cholestadienyl monosulfonic acid is what color?

A

Green complex

42
Q

Use of chlorform and sulfuric acid as reagent is in what method?

A

Salkowski

43
Q

70% of lipids in blood esterified and 30% are free cholesterol. True or False?

A

True

44
Q

This falsely increase results in enzymatic reaction sequence

A

Oxidizing agents such as soaps

45
Q

This falsely decrease results in enzymatic reaction sequence

A

Reducing agent such as ascorbic acid

46
Q

HDL measurement requires removal of TG and VLDL. True or False?

A

False, it requires removal of LDL and VLDL

47
Q

LDL-Cholesterol and VLDL-Cholesterol can be precipitated using polyanions like

A

Dextran sulfate magnesium
Heparin with manganese
Phosphotungstate with magnesium

48
Q

Formaldehyde + chromotropic acid → red
chromophore is the principle of what TG method?

A

Van Handel Zilversmith

49
Q

Acidic buffer in VHZ results to ?

A

Red chromophore

50
Q

Alkaline buffer in VHZ results to ?

A

Blue chromophore

51
Q

Formaldehyde + diacetyl acetone → diacetyl lutidine is the principle of what TG method?

A

Hantzsch Condensation

52
Q

Diacetyl lutidine is what color?

A

Faint green

53
Q
A