(Laboratory) Identification of Staphylococcus Flashcards
List down the step-by-step procedure in the Collection of the Bacterial Sample for Staphylococcus.
- Use sterile cotton swab to collect bacterial sample in the skin/nasal cavity
- Inoculate the sample in MSA
- Incubate at 37C for 18-24 hours
- Observe for growth and color development
What do we use to collect a bacterial sample for the patient?
Sterile Cotton Swab
Site where we collect the bacterial sample from the patient.
Skin or Nasal Cavity
What medium is used to inoculate the sample from the collected bacterial sample?
MSA or Mannitol Salt Agar
Incubation period from the collected bacterial sample in MSA.
37C for 18-24 hours
Positive result in the Collection of Bacterial Sample
Yellow colonies
List down the step-by-step procedure in the Colonial Morphology.
- Get an inoculum and streak the BAP using multiple interrupted streak method
- Incubate at 37C for 18-24 hours
- Examine the colonies and describe
Medium used in the Colonial Morphology.
BAP (Blood Agar Plate)
NA (Nutrient Agar)
Incubation period for Colonial Morphology.
37C for 18-24 hours
List down the step-by-step procedure in the Cell Morphology on Gram stain.
- Using a sterile loop, place a loopful of saline into a labelled slide
- Emulsify a colony into the saline
- Air dry and heat fix
- Stain with gram stain
- View under microscope (OIO)
What is seen on Cell Morphology on Gram Stain of Staphylococcus?
Gram positive cocci in clusters
List down the step-by-step procedure in the Oxidase Test.
- Using a sterile loop/applicator stick, rub a colony into the oxidase strip
- Observe for color change
What is the positive result in the Oxidase Test?
Purplish to black
What is the negative result in the Oxidase Test?
No color change
List down the step-by-step procedure in the Catalase Test.
- Place 1 drop of 3% hydrogen peroxide reagent in a glass slide
- Emulsify a colony using applicator stick
- Observe
How many drops of the 3% hydrogen peroxide reagent is added in the Catalase Test procedure?
1 drop
Reagent used in the Catalase Test.
3% hydrogen peroxide reagent
What is the positive result in the Catalase Test?
Bubble Formation
What is the negative result in the Catalase Test?
No gas bubbles
List down the step-by-step procedure in the DNAse Test.
- Using bacteria from a plate, inoculate the DNAse medium in a straight line
- Incubate the DNAse medium for 18-24 hours
- After incubation, add a small amount of 1N HCl to the plate, flooding the colonies.
- Discard the excess acid and observe the media surrounding the colonies.
Incubation period in the DNAse Test.
18-24 hours
What is added to the plate of the DNAse medium after the incubation?
1N HCl (1 Normal Hydrochloric acid)
Positive result in the DNAse Test.
Clearing surrounding the colonies
Screening Coagulase Test
Slide Coagulase
Confirmatory Coagulase Test
Tube Coagulase Test
What is added on the “T” portion on the slide of the Slide Coagulase Test?
Plasma
What is added on the “C” portion on the slide of the Slide Coagulase Test?
Saline
The “T” on the slide for the Slide Coagulase Test means _____
Test
The “C” on the slide for the Slide Coagulase Test means _____
Control
Positive result in the Slide Coagulase Test
Clumping
Negative result in the Slide Coagulase Test
No Clumping
A negative control in the Slide Coagulase Test should show (clumping or no clumping).
No clumping
List down the step-by-step procedure in the Tube Coagulase Test.
- Add 0.5 mL of plasma into a tube
- Inoculate a loopful of bacteria
- Incubate for 2 hours and observe for clot formation
- If no clot is observed, incubate for another 2 hours and observe for clot formation
- If no clot is observed after a total of 4 hours incubation, incubate further up to 18-24 hours
Specimen used in the Tube Coagulase Test (In Laboratory Setting)
0.5 mL of Plasma