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Clinical Bacteriology (Finals) > (Laboratory) Identification of Staphylococcus > Flashcards

(Laboratory) Identification of Staphylococcus Flashcards

1
Q

List down the step-by-step procedure in the Collection of the Bacterial Sample for Staphylococcus.

A
  1. Use sterile cotton swab to collect bacterial sample in the skin/nasal cavity
  2. Inoculate the sample in MSA
  3. Incubate at 37C for 18-24 hours
  4. Observe for growth and color development
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2
Q

What do we use to collect a bacterial sample for the patient?

A

Sterile Cotton Swab

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3
Q

Site where we collect the bacterial sample from the patient.

A

Skin or Nasal Cavity

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4
Q

What medium is used to inoculate the sample from the collected bacterial sample?

A

MSA or Mannitol Salt Agar

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5
Q

Incubation period from the collected bacterial sample in MSA.

A

37C for 18-24 hours

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6
Q

Positive result in the Collection of Bacterial Sample

A

Yellow colonies

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7
Q

List down the step-by-step procedure in the Colonial Morphology.

A
  1. Get an inoculum and streak the BAP using multiple interrupted streak method
  2. Incubate at 37C for 18-24 hours
  3. Examine the colonies and describe
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8
Q

Medium used in the Colonial Morphology.

A

BAP (Blood Agar Plate)
NA (Nutrient Agar)

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9
Q

Incubation period for Colonial Morphology.

A

37C for 18-24 hours

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10
Q

List down the step-by-step procedure in the Cell Morphology on Gram stain.

A
  1. Using a sterile loop, place a loopful of saline into a labelled slide
  2. Emulsify a colony into the saline
  3. Air dry and heat fix
  4. Stain with gram stain
  5. View under microscope (OIO)
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11
Q

What is seen on Cell Morphology on Gram Stain of Staphylococcus?

A

Gram positive cocci in clusters

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12
Q

List down the step-by-step procedure in the Oxidase Test.

A
  1. Using a sterile loop/applicator stick, rub a colony into the oxidase strip
  2. Observe for color change
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13
Q

What is the positive result in the Oxidase Test?

A

Purplish to black

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14
Q

What is the negative result in the Oxidase Test?

A

No color change

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15
Q

List down the step-by-step procedure in the Catalase Test.

A
  1. Place 1 drop of 3% hydrogen peroxide reagent in a glass slide
  2. Emulsify a colony using applicator stick
  3. Observe
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16
Q

How many drops of the 3% hydrogen peroxide reagent is added in the Catalase Test procedure?

A

1 drop

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17
Q

Reagent used in the Catalase Test.

A

3% hydrogen peroxide reagent

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18
Q

What is the positive result in the Catalase Test?

A

Bubble Formation

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19
Q

What is the negative result in the Catalase Test?

A

No gas bubbles

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20
Q

List down the step-by-step procedure in the DNAse Test.

A
  1. Using bacteria from a plate, inoculate the DNAse medium in a straight line
  2. Incubate the DNAse medium for 18-24 hours
  3. After incubation, add a small amount of 1N HCl to the plate, flooding the colonies.
  4. Discard the excess acid and observe the media surrounding the colonies.
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21
Q

Incubation period in the DNAse Test.

A

18-24 hours

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22
Q

What is added to the plate of the DNAse medium after the incubation?

A

1N HCl (1 Normal Hydrochloric acid)

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23
Q

Positive result in the DNAse Test.

A

Clearing surrounding the colonies

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24
Q

Screening Coagulase Test

A

Slide Coagulase

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25
Q

Confirmatory Coagulase Test

A

Tube Coagulase Test

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26
Q

What is added on the “T” portion on the slide of the Slide Coagulase Test?

A

Plasma

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27
Q

What is added on the “C” portion on the slide of the Slide Coagulase Test?

A

Saline

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28
Q

The “T” on the slide for the Slide Coagulase Test means _____

A

Test

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29
Q

The “C” on the slide for the Slide Coagulase Test means _____

A

Control

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30
Q

Positive result in the Slide Coagulase Test

A

Clumping

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31
Q

Negative result in the Slide Coagulase Test

A

No Clumping

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32
Q

A negative control in the Slide Coagulase Test should show (clumping or no clumping).

A

No clumping

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33
Q

List down the step-by-step procedure in the Tube Coagulase Test.

A
  1. Add 0.5 mL of plasma into a tube
  2. Inoculate a loopful of bacteria
  3. Incubate for 2 hours and observe for clot formation
  4. If no clot is observed, incubate for another 2 hours and observe for clot formation
  5. If no clot is observed after a total of 4 hours incubation, incubate further up to 18-24 hours
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34
Q

Specimen used in the Tube Coagulase Test (In Laboratory Setting)

A

0.5 mL of Plasma

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35
Q

Positive result in the Tube Coagulase Test

36
Q

Negative result in the Tube Coagulase Test

A

No Coagulum

37
Q

An enzyme that catalyzes the release of oxygen from hydrogen peroxide (H2O2)

38
Q

A test that demonstrate the presence of catalase

A

Catalase Test

39
Q

Reagent used for the routine culture of Catalase Test

40
Q

Reagent used for the detection of catalase in anaerobe

41
Q

An enzyme-like protein and causes plasma to clot by converting fibrinogen to fibrine

42
Q

Best single pathogenicity test for Staphylococcus aureus

A

Coagulase Test

43
Q

Specimen used in the Slide Method of Coagulase Test? (According to the Recorded Lecture)

44
Q

A method under the Coagulase Test that detects bound coagulase

A

Slide Method

45
Q

Positive result in the Slide Method of Coagulase Test

A

Agglutination

45
Q

Positive result in the Slide Method of Coagulase Test

A

Agglutination

45
Q

Positive result in the Slide Method of Coagulase Test

A

Agglutination

46
Q

Specimen used in the Tube Method of Coagulase Test? (According to the Recorded Lecture)

A

0.5 mL rabbit’s plasma

47
Q

A method under the Coagulase Test that detects unbound coagulase (staphylocoagulase)

A

Tube Method

48
Q

Positive result in the Tube Method of the Coagulase Test

A

Coagulum/Clot

49
Q

Medium used in the Oxidation-Fermentation (O/F) Reactions Test

A

O/F Glucose Medium or the Hugh and Leifson’s Medium

50
Q

How many tubes are needed in the Oxidation-Fermentation (O/F) Reactions Test?

51
Q

Describe the 2 tubes in the Oxidation-Fermentation (O/F) Reactions Test.

A

1st Tube: Overlaid with mineral oil or melted paraffin (Anaerobic Environment)
2nd Tube: Left open in the air (Aerobic Environment)

52
Q

Oxidative utilization of the carbohydrate will result in acid production or color yellow in _____

A

Open tube only

53
Q

Fermentative utilization of the carbohydrate will result in acid production or yellow in _____

A

Both open and closed tubes

54
Q

pH indicator in the Oxidation-Fermentation (O/F) Reactions Test

A

Bromthymol blue

55
Q

The pH indicator bromthymol blue changes into color _____ when it detects acid

56
Q

In the Oxidation-Fermentation (O/F) Reactions Test, if the closed tube is positive, what does it indicates?

A

Yellow Color
Fermentative

57
Q

In the Oxidation-Fermentation (O/F) Reactions Test, if the open tube is positive, what does it indicates?

A

Yellow Color
Oxidative

58
Q

In the Oxidation-Fermentation (O/F) Reactions Test, if both the open and closed tube is positive, what does it indicates?

A

Staphylococci specie

59
Q

In the Oxidation-Fermentation (O/F) Reactions Test, if the open tube is positive and the closed tube is negative, what does it indicates?

A

Micrococcaceae specie

60
Q

Active chemical component in the Modified Oxidase Test

A

Tetramethyl-para-phenylenediamine dihydrochloride

61
Q

A reagent impregnated disc recommended for use in qualitative procedures to aid in the differentiation of Staphylococcus and Micrococcus by the detection of the oxidase enzyme.

A

Microdase disc

62
Q

Positive result in the Modified Oxidase Test

A

Blue to purple to black complex
Micrococci specie

63
Q

Negative result in the Modified Oxidase Test

A

No color change
Staphylococcus

64
Q

Medium used in the Mannitol Fermentation Test

A

MSA (Mannitol Salt Agar)

65
Q

Positive result in the Mannitol Fermentation Test

A

Yellow color
Staphylococcus aureus

66
Q

Negative result in the Mannitol Fermentation Test

A

Pink color
Coagulase-negative Staphylococci specie

67
Q

pH indicator in the Mannitol Fermentation

A

Phenol red

68
Q

Phenol red turns _____ when it detects acid

69
Q

Medium used in the DNAse Test

A

DNAse medium

70
Q

Positive result in DNAse Test

A

Staphylococcus aureus

71
Q

Negative result in DNAse Test

A

Staphylococcus epidermidis

72
Q

A test used to determine the ability of an organism to hydrolyze DNA and utilize it as a source of carbon and energy for growth.

A

DNAse Test

73
Q

Medium used in the Gelatin Liquefaction/Hydrolysis Test

A

12% Gelatin Medium

74
Q

Incubation period in Gelatin Liquefaction/Hydrolysis Test

A

35ºC for 16-18 hours

75
Q

Positive result in Gelatin Liquefaction/Hydrolysis Test

A

Staphylococcus aureus

76
Q

Negative result in Gelatin Liquefaction/Hydrolysis Test

A

Staphylococcus epidermidis

77
Q

A protein derived from the connective tissues of vertebrates that is collagen

78
Q

These are proteases secreted extracellularly by some bacteria which hydrolyze or digest gelatin.

A

Gelatinases

79
Q

Staphylococcus aureus is coagulase (positive or negative)

A

Coagulase positive

80
Q

Staphylococcus epidermidis is coagulase (positive or negative)

A

Coagulase negative

81
Q

Staphylococcus saprophyticus is coagulase (positive or negative)

A

Coagulase negative

82
Q

Staphylococcus aureus is novobiocin (susceptible or resistant)

A

Novobiocin susceptible

83
Q

Staphylococcus epidermidis is novobiocin (susceptible or resistant)

A

Novobiocin susceptible

84
Q

Staphylococcus saprophyticus is novobiocin (susceptible or resistant)

A

Novobiocin resistant

85
Q

Sugar fermentation is of no diagnostic value except for _____, which is usually fermented anaerobically by Staphylococcus aureus but not by other species

Clinical Bacteriology (Finals) (4 decks)

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