Laboratory Glasswares and Plasticwares Flashcards
Identify whether it is a glassware or a plasticware:
reusable
glassware
Identify whether it is a glassware or a plasticware:
disposable
plasticware
Identify whether it is a glassware or a plasticware:
highly shock proof
plasticware
Identify whether it is a glassware or a plasticware:
breakable
glassware
Identify whether it is a glassware or a plasticware:
ideal for acidic solutions
glassware
Identify whether it is a glassware or a plasticware:
high thermal and corrosion resistance
glassware
Identify whether it is a glassware or a plasticware:
ideal for alkaline solutions
plasticware
Identify whether it is a glassware or a plasticware:
relatively inert
plasticware
Identify whether it is a glassware or a plasticware:
good optical properties
glassware
Identify whether it is a glassware or a plasticware:
flexible
plasticware
Identify whether it is a glassware or a plasticware:
low cost
glassware
Identify whether it is a glassware or a plasticware:
for cryogenic experiments
plasticware
Enumerate all the types of glasswares
- high thermal resistant glass
- high silica glass
- glass with high resistance to alkalis
- low actinic glass
- standard flint glass
Enumerate all the types of plasticwares
- polyolefins: polyethylene, polypropylene
- polycarbonate resin
- Tygon
- Teflon fluorocarbon resins
Chemical resistance part
- polystyrene
- polyethylene
- polypropylene
- Teflon
- polycarbonate
Aside from the mentioned glasswares, cite other lab glasswares.
- buret or biuret
2. cuvette
Enumerate other lab glass vessels
- volumetric flask
- graduated cylinders
- Erlenmeyer flask and griffin beaker
what type of material is a high thermal resistant glass?
borosilicate glass with low alkali content
what glassware resists heat, corrosion, and thermal shock?
high thermal
resistant glass
what are the most common high thermal resistant glass?
- pyrex
- Klimax
- Exax
6x stronger than borosilicate
glass
corex
Corex is a type of high thermal resistant glass and it is also known as…
alumina-silicate glass
Type of high thermal resistant glass that is able to resist clouding due to alkali or scratching
corex
Type of high thermal resistant glass that is ideal for higher temperature thermometers, graduated cylinders, and centrifuge tubes
corex
Type of high thermal resistant glass that is recommended for use in application involving high temperature and drastic heat shock and extreme chemical treatment with acids and dilute alkali
Vycor
Type of high thermal resistant glass that is ideal for ashing and ignition techniques
vycor
Vycor is heatable to ___C and withstand down shock from ____C to ___ water
900C; 900C to ice water
it has a 96% silica content comparable to fuse quartz in its thermal endurance, chemical stability, and electrical characteristics
high silica glass
high silica glass has a __% silica content
96%
it is radiation resistant, good optical qualities, and temperature capabilities
high silica glass
ideal for high precision analytical work and can
also be used for optical reflectors and mirrors
high silica glass
known as the soft glass
glass with high resistance to alkalis
boron-free glassware for strong alkali solution
and digestion with strong alkalis
glass with high
resistance to
alkalis
glass with high
resistance to
alkalis is a ___-free glassware
boron-free glassware
it has a less thermal resistant than borosilicate glass
glass with high
resistance to
alkalis
this glassware is amber-colored
low-actinic glass
why is the low-actinic glass amber colored?
- to reduce the amount of light passing through the substance within the glassware - To avoid exposing to light which will lead to degradation, hence, leading to false results
highly protective laboratory glassware for
handling heat-labile substances in the 300-
500nm range (bilirubin, carotene, and Vit. A)
low actinic glass
heat-labile substances that low actinic glass handle
bilirubin
carotene
Vit. A
standard flint glass is also known as
soda lime glass
Standard flint glass is composed of a mixture of oxides: __, __, and __
Si, Ca and Na
lowest in cost readily fabricated
standard flint glass
poorly resistant to high temperature and sudden
changes in temperature
standard flint glass
easy to melt and shape thus used as reagent
bottles and disposable laboratory glassware
standard flint glass
release alkali into the pipetted liquid
standard flint glass
beginning to replace glassware in the lab
plasticware
unique high resistance to corrosion and
breakage as well as its varying flexibility
plasticware
relatively inexpensive
▪ allowing most items to be completely
disposable after its use
plasticware
polyethylene and polypropylene falls under the group of
polyolefins
unique group of resins with relatively inert
chemical properties
polyolefins
polyolefins is unaffected by:
- acids (howv, conc. sulfuric acid slowly attacks polyethylene at room temp)
- alkalis
- salt solutions
- most aqueous soln
Hydrocarbons that can cause moderate swelling at RT of polyolefins
- aromatic
- aliphatic
- chlorinated
Substances that can slowly penetrate polyolefins plastics?
- organic acids
- essential oils
- halogens
strong oxidizing agents attack this group at
elevated temperatures only
polyolefins
what agents attack polyolefins at an elevated temperature only?
strong oxidizing agents
At what temp does strong oxidizing agents attack polyolefins?
elevated temp only
which is more expensive, polypropylene or polyethylene?
polypropylene
sterilizable however, it absorbs pigment and tend to become discolored
polypropylene
twice as strong as polypropylene (from -100C to +160C)
polycarbonate resin
can endure thermal shock
polycarbonate resin
chemical resistance is not as wide as the
polyolefin
polycarbonate resin
unsuitable for use with bases as amines,
ammonia, and alkalis as well as oxidizing
agents
polycarbonate resin
polycarbonate resin is unsuitable for use with?
- bases: amines, ammonia and alkalis
- oxidizing agent
glass-clear and shatter proof
polycarbonate resin
ideal for centrifuge tubes & graduated cylinders
polycarbonate resin
why is polycarbonate resin ideal for centrifuge tubes & graduated cylinders?
because it is glass clear and shatter proof
non-toxic, clear plastic of modified PVC
tygon
meaning of PVC
polyvinylchloride
flexible and used to handle most chemicals
tygon
tubing is soft and flexible and quickly slips over
tabulatures but gripping tightly on glass or
metals
tygon
almost chemically inert with high corrosion
resistance at extreme temperatures
Teflon fluorocarbon resins
for cryogenic experiments and work at high
temperatures over extended periods
Teflon fluorocarbon resins
pure translucent white and inert
Teflon fluorocarbon resins
easy to clean and fast drying but it can be
scratched and misshaped
Teflon fluorocarbon resins
type of plastic that is useful with water and aqueous salt solutions?
polystyrene
polystyrene is not recommended for use with?
- acids
- aldehydes
- ketones
- ethers
- hydrocarbons
- essential oils
In polystyrene plastic, alcohols and bases can be used, but storage beyond __hrs is discouraged
24 hrs
alcohols and bases can be used, but storage
beyond 24 hours is discouraged
polystyrene
what are the classifications of polyethylene?
conventional and linear
conventional and linear polyethylene have different chemical resistances
False; similar chemical resistances
both classifications of polyethylene (i.e.,
conventional and linear) have similar chemical
resistances: they have excellent chemical
resistance to most substances, with the
exception of:
▪ aldehydes ▪ amines ▪ ethers ▪ hydrocarbons ▪ essential oils
For ________ polyethylene, the exceptions
should also include:
▪ lubricating oil
▪ silicones
conventional polyethylene
conventional polyethylene is not chemical resistant to?
▪ aldehydes ▪ amines ▪ ethers ▪ hydrocarbons ▪ essential oils ▪ lubricating oil ▪ silicones
the usage of any of the above-named chemical
groups in polyethylene should be limited to __ hours at ___
temperature
24 hrs; room temp
has the same chemical resistance as linear
polyethylene
polypropylene
this resin possesses excellent chemical resistance
to almost all chemicals used in the clinical lab
teflon
very susceptible to damage by most chemicals
polycarbonate
for a long period of time, polycarbonate is resistant to?
water
aqueous salts
food
inorganic acids
Resistance to chemicals decreases as?
the temperature of the resin nears its maximum
Chemical resistance will also vary as the?
concentration of the chemical increases
is calibrated to hold one exact volume of
liquid (TC)
volumetric flask
are long cylindrical tubes usually help
upright by an octagonal or circular base
graduated cylinders
designed to hold different volumes rather
than one exact amount
Erlenmeyer flask and griffin beaker
used for titration and for dispensing aliquots
of a solution
buret or biuret
high-silica glassware
cuvette
used to hold solutions for optical reading of
absorbance
cuvette
what are the two types of cuvette?
- round cuvette
- square cuvette
how do you clean glassware and plasticware?
- glass or plastic that is in direct contact with hazardous material
is usually disposable, otherwise, it must be decontaminated
(special techniques may be required for certain situations) - pre-soaking glassware in soapy water
- visual inspection should reveal spotless vessel walls
- some applications may require plastic rather than glass because
glass can absorb metal ions
in what instances during cleaning that some applications may require plastic rather than glass?
glass can absorb metal ions
How to wash glasswares?
- Wash in tap water to remove gross dirt
- Soak in suitable detergent for a few minutes
- Soak in running tap water until no trace detergent is left. 3 times
- Rinse with distilled water to remove any contaminants present in the detergent
- Dry in an oven at temperature less than 140C
How to wash glassware with blood clots?
10% Sodium Hydroxide
Soak for 12-24 hours, then follow routine procedure. Dry micropipettes using an acetone rinse
How to wash new pipettes (SI alkaline)
Rinse with 5% hydrochloric acid or 5% nitric acid. Wash following routine procedure
How to wash glassware with metal ion determination?
Acid soak (20% nitric acid) for 12-24 hrs. Rinse with distilled water 3-4 times. Water should be fresh for each rinsing step. Dry
How to wash glassware with grease?
- Soak in any organic solvent
- Dissolve 100g potassium hydroxide in 100 mL distilled water. Allow to cool. Add 900 mL commercial-grade
10% ethanol. ❗❗ Not to be used for delicate glassware. ❗❗ - Contrad 70 (manufactured by Harleco)
How to wash glasswares with permanganate stains?
- 50% hydrochloric acid. Rinse with tap water. Wash.
2. Dissolve 1% ferrous sulfate in 25% sulfuric acid.
How to wash bacteriologic glassware?
Soak in 2% to 4% cresol solution followed by autoclaving and thorough washing
how to wash glasswares with iron determination?
Soak in 1:2 dilution of concentrated HCl solution or 1:3 dilution of concentrated HNO3.