Laboratory Exercise 3: Differential Stains Flashcards
Staining
Are used to make this minute organism visible, display its structure in the microscope, reveal their chemical nature and influence its growth and development.
3 different methods of staining:
Simple, Differential, Selective Staining
This is employed to study the morphology of the bacteria in films with simple dyes, direct staining.
Simple Staining
This staining method uses one dye only.
Simple Staining
Stained microorganisms with unstained background, uses Methylene blue
Positive Staining
Unstained environment with stained background, uses India ink
Negative Relief Staining
This is used to show by special staining methods and reveal the physiochemical characteristics and differences among bacteria that aid in their description and classification, two or more dye employed.
Differential Staining
This staining method uses two/more dyes.
Differential Staining
This dye initially stains the bacterial cell.
Primary Stain
Enhances the affinity of the primary stain to the organism
Mordant
Most critical stage, because this will differentiate bacteria based on its cell wall composition that reacts to the primary stain used.
Decolorizer
Stain the background or stain the organism not reactive to the primary stain.
Counter Stain
This is used to present bacterial spores, capsules, and flagella by appropriate staining method of different organism.
Selective Staining
Spore Staining
Modified Writz Method
Capsule Staining
Modified Maneval’s Staining
