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Hematology 2 Lab > LABORATORY ACTIVITY 9 > Flashcards

LABORATORY ACTIVITY 9 Flashcards

1
Q

 RBC is (normocytic)

A

6-8 mm

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2
Q

 Lower than 6 =

A

microcytic

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3
Q

 Higher than 8=

A

macrocytic

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4
Q

 Normal shape:

A

biconcave discoid

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5
Q

 If there are spikes it can be:

A

acanthocytes or spur cells

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6
Q

 Normal color of RBC:

A

Pink to orage or salmon pink

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7
Q

 Normal color of Reticulocyte:

A

Pinkish gray or blue-gray

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8
Q

INCLUSIONS ● We can see

A

malarial parasites, basophilic stippling, howell jolly bodies, heinz bodies

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9
Q

PURPOSE:
1. Serve as a [?] to verify the result produced by automated analyzers.
2. Shows generalized B, problem that leads to [?]

A

quality control

anemia

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10
Q

○ Ex. you are wondering why RBC is very low so you prepared a smear and you saw that there are acanthocytes, spherocytes and other abnormal cells, so we do this to verify and to compare the results with that of the [?]

A

automation

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11
Q

○ Remember the principle of automation is [?]. So it detects using the size of the RBCs. If RBC is smaller or bigger than normal, it will not count those RBCs. Leading to low RBC count.

A

electrical impedance and light scattering

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12
Q

o Especially if there are problems in WBC and Platelets. Why? For example, we have hypersegmented WBC, so maybe it is not only a problem with the WBC but also in the RBC like [?].

A

anemia

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13
Q

o Hypersegmented WBC also exist in [?] and both of these deficiencies can cause anemia. So maybe there is a problem with the erythropoiesis of the bone marrow.

A

Vit. B12 deficiency and folate deficiency

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14
Q

o [?] are important for the maturation of RBC in the BM.

A

Folate and Vit. B12

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15
Q

o If there is decreased platelet, it is

A

acute ammonia

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16
Q

PREPARATION OF WEDGE SMEAR
● [?] drop of blood
● [?] angle for rodaks and (25-40) angle for steninger

A

2-3 mm

30-45

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17
Q

QUALITY
● Film is [?] of the slide
● [?], buller form is not allowed
● [?] edge (no dropping marks)
● Without [?]
● [?] when exposed to light
● In stain blood cell examination, we are going to read until the [?] of the stained sample.

A

⅔ to ¾

Finger shaped/ tongue shaped

Feathery

holes/irregularities

Rainbow

center

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18
Q

FACTORS AFFECTING A GOOD SMEAR

A

(P) Pressure

(A) Angle

(S) Size

(S) Speed

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19
Q

(P) Pressure

Thick:
Thin:

A

Decrease
Increase

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20
Q

(A) Angle

Thick:
Thin:

A

Increase
Decrease

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21
Q

(S) Size

Thick:
Thin:

A
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22
Q

(S) Speed

Thick:
Thin:

23
Q

● Romanowsky stain (?) : primary or contrasting stain

A

Wright stain/ Wright Giemsa

24
Q

● [?] (has many colors)

A

Polychrome stain

25
Q

● stains RNA (acidic): [?]

A

Free methylene blue

26
Q

● stains Hemoglobin (basic) and eosinophilic granules (basic): [?]

A

Free eosin or eosin y

27
Q

which is negatively charged and acidic

A

Free eosin or eosin y

28
Q

which is positively charged and basic

A

Free methylene blue

29
Q

Thick films

A

EXCESSIVE BLUE STAIN

30
Q

Insufficient staining time

A

EXCESSIVE PINK

31
Q

Prolonged staining time

A

EXCESSIVE BLUE STAIN

32
Q

Prolonged washing time

A

EXCESSIVE PINK

33
Q

Inadequate washing

A

EXCESSIVE BLUE STAIN

34
Q

High acidity of stain

A

EXCESSIVE PINK

35
Q

Too high alkalinity of buffer

A

EXCESSIVE BLUE STAIN

36
Q

Mounting coverslip before the slide is dry

A

EXCESSIVE PINK

37
Q

You only mount a coverslip when you are going to preserve

A

EXCESSIVE PINK

38
Q

 side to side

A
  1. CROSS SECTIONAL
39
Q

 tail towards the head

A
  1. LONGITUDINAL METHOD
40
Q

 reference and the preferred method, so first vertically then horizontally

A
  1. BATTLEMENT METHOD
41
Q

 we are going to count three consecutive horizontal edge fields, so two fields is towards the center and two fields horizontally and two fields vertically

A
  1. HORIZONTAL EDGE
42
Q

Abnormal size =

43
Q

Abnormal shape =

A

Poikilocyte

44
Q

Normal color of RBC: Salmon pink (?)

A

normal Hb synthesis

45
Q

● Decreased color w/ increased central pallor =

A

Hypochromic

46
Q

● Increased color w/ decreased central pallor =

A

Hyperchromic

47
Q

● Normal: central pallor

48
Q

(failure to degrade RNA in the retic due to deficiency in Pyrimidine 59 nucleotidase

A

basophilic stippling

49
Q

Distance from the edge of the slide

50
Q

Wright Giemsa (?)

A

Carbol Fuschin and Methylene Blue

51
Q

a polychrome stain: has two colors staining [?]

A

cytoplasm and nucleus

52
Q

Laboratory Activity 9: STAINED BLOOD CELL EXAMINATION

Specimen Required:

A

Stained blood smears (CSR Control slides)

53
Q

STAINED BLOOD CELL EXAMINATION

  1. Using oil immersion objective, study a thin area of the blood smear. Cells must be (?).
  2. Examine about (?) microscopic fields and make observations of:
    (?)
  3. Report the degree of anisocytosis and/or poikilocytosis following the standard manner of reporting
    as (?)
A

evenly distributed and
free of stain precipitates, not overlapping nor bunched together

15

a. RBCs’ hemoglobin content, size, shape, and presence of inclusion bodies.
b. WBCs’ cytoplasm and nucleus
c. Platelets’ size, shape and disrtibution

  • ; +1, +2, +3 or +4

Hematology 2 Lab (7 decks)

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