Laboratory Flashcards

1
Q

Importance of diagnostics

A

Find cause
Compare results
Change care
Targeted treatment

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2
Q
Ocular/eye piece
Objective lens
Iris diaphragm
Substage condenser
Coarse focus
Fine focus
Rheostat
A
...
Magnifies (low x4/10, high x40/100)
Controls the amount of light let through
Gathers the light and concentrates it into a cone of light which illuminates specimen
Brings object into focus
Refines image.
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3
Q
Hazardous waste regulations
Year
Register with
Cosignment note kept for
Inspected by
Lists?
A
2005
Gov annually
3 years
HSE
Waste products deemed hazardous by waste framework directive
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4
Q

Collection and disposal of waste regulations
Year
Replaces
Incineration our holding waste requires

A

1988
Control of pollution act 1974
Disposal licence

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5
Q
Centrifuge
Spins at?
Used for?
Plasma
Buffy coat
Clot
A

Separates liquids into different densities
10,000RMP / 5 minutes
Haematocrit, biochemistry, urine, faecal egg count
Fluid in which cellular components are suspended in
WBC
RBC

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6
Q

Microscope
Oil immersion
Main scale
Vernier scale

A

X100, refracts light
Attached to stage
Moves with stage

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7
Q
Refractometer
Used for
Callibrate to
Ppe
Lower the spg
Higher
Dog
Cat
Horse
A
Spg, total solid
1.000 (distilled water)
Gloves, apron
More dilute
Concentrated
1.015-1.045
1.035-1.060
1.020-1.050
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8
Q

Analysers
Haematology
What it does?
Anticoagulant used?

A

Analysis of components of the blood

Ethylenediaminetetraacetic acid

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9
Q
Analysers
Biochemistry
What it does?
Anticoagulant used?
Dry analyser
Wet analyser
Serum=
A
Analysis of components of plasma
Lithium heparin
Colour change due to light
Colour change due to chemical
Plasma - clotting agents
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10
Q
Analysers
Electrolyte + blood gas analyser
What it does?
Anticoagulant
Gives info on
A

Tells PH of the blood
Lithium heparin
potassium, sodium, chloride

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11
Q
Needles
16g
18g
19g
20g
21g
22g
23g
24g
25g
26g
Iv dog, cat, horse
Im dog, cat, horse
A
White
Pink
Cream
Yellow
Green
Black
Blue
Purple
Orange
Brown
21/23, 23,25, 20
21/23, 23, 18
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12
Q

Large amounts of pressure in the syringe cause
Colour?
Yellow serum =
Milky white =

A

Haemolysis
Pink / red
Icteric (bilirubin)
Lipaemic (fat, unstarved)

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13
Q

Sample preservation
Plasma proteins act as a
Thrombin is a
Anticoagulants block

A

Catalyst for conversion of prothrombin to thrombin (thromboplastin)
Calcium and a clotting factor which converts fibrinogen into fibrin
Calcium

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14
Q
Blood tubes
EDTA
Sodium fluoride/potassium oxalate
Lithium heparin
Sodium citrate
Plain
Serum
A

Pink cap, mauve vac, haema, pcv, blood smear,
Yellow top, grey vac, glucose estimate
Orange top, green vac, biochem, Electrolytes
Blue top, blue vac, clotting profile
White top, red vac, urine
Brown top, red vac, serum, bile acids, biochem

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15
Q
Urine
Better to catch when
Types of collection
Run within?
Refrigerated for
Transport sample
A

Midstream
Free flow, catheter, cystocentesis, manual expression,
1 hour
6 hours plain container
Boric acid, hydrochloric acid, thymol, acetic acid- red top

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16
Q
Faeces
Lid colour
Equine rectal sample
Refrigerated
E. Coli
A

Blue, 50g max
Vet
48 hours max
Gram - rod

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17
Q

Skin and hair

Preservative

A

Liquid paraffin

Potassium hydroxide

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18
Q

Secretions
Common preservative
Transport for virus

A

Aimes charcoal

Glasgow virus

19
Q

Tissue preservation

Preservative

A

Formalin saline 10x sample

Fixes sample

20
Q
Cerebrospinal fluid
Found?
Perform when
2 sample points
Diagnose
Maintain
Limbs restrained cranially during Lumbar by
Tube colour
A

Clear, colourless liquid
Cushions the brain and Spinal chord (subarachnoid space + ventricular system)
Ultrafiltrate (choroid plexus)
CNS issue nothing else worked
Cisternal (non collapsing et tube, 1.5inch)
Lumbar (2-3.5inch)
Meningitis, infection, tumour (protein, glucose)
Axis occipital bone alignment
Flexing coxofemoral joints
EDTA 1ml/5kg

21
Q
Bone marrow
Greater tubercle of humerus
Iliac crest
Intratrochantic fossa of femur
Cytology (saline)
   Leukaemia
   Myeloma
Histopathology (formuline)
   Myelofibrosis
   Metastatic neoplasia
   Lymphoma
A
Dog
Dog
Cat
Cancer of dysplasia (change to blood cells)
Cancer of maturation defect to plasma cells
Defect in production of blood cells
Spreading of cancer
Cancer of lymphatic system
22
Q

Synovial fluid
What is it?
Found where?
Tubes used

A

Viscous fluid which reduces friction
Radiocarpal, intercarpal, carpal sheath
Plain or EDTA

23
Q

Fine needle aspiration
Who can do?
Needle, syringe
Vaccum

A

Rvn
25g, 2.5ml
2-4ml

24
Q

Equine tracheal wash / BAL

Tube colour

A

EDTA/plain

5-10ml warmed saline

25
Pericardiocentesis Relieves? Thrombocentesis Enters? Abdominal paracentesis Removes? Tube
Cardiac tamponade Thoracic cavity Peritoneal fluid (ascites) EDTA, pain tube
26
``` Haemolysis Lysis Turgid Osmosis Permeability Isotonic Hypotonic Hypertonic Crenation ```
``` Breakdown of red blood cells Breakdown down of a cell membrane Swelling of a cell (hypotonic) Movement of water from a lower concentration to a higher concentration through a semi Permeable membrane Tinicity and osmotic pressure the same Lower concentration than the cell Higher concentration than the cell Shrinking (hypertonic) ```
27
Storage and transportation Maintain temp at ``` Packaging and labelling Classified by Category A Catergory B Withstand pressure of Label Occult blood ```
``` 2-8 degrees Potential harm Infectious substance that causes fatal disease to human or animals UN2814 - HUMANS UN2900 - ANIMALS infectious substance UN3373 - diagnostic speciemens 95pka Delivery, senders address, emergency contact, UN no, hazard warning Hidden blood ```
28
ELISA Detects antibody, antigen Foal immunoglobulins (igg) Absorption? Transfusion?
Enzyme linked immunoabsorbant assay Impregnated with antigens which antibodies stick to (colour change) Detects prescence of antigen directly Failure of passive transfer from colostrum Absorption of antibodies 6-8 hours. Stops at 24/36hrs <400mg/dl
29
``` Haematopoiesis Haemophilia Leukopenia Leucocytosis Penia Philia/cytosis Rouleux Reticulocyte Hypochromasia Howell-jolly body Heinz body Aggulanation ```
``` Production of blood cells Impaired ability to make blood clots Deficiency of white blood cells Increase in white blood cells Deficiency Increase/decrease in cells Aggregation of RBC in chains Immature RBC RBC with lack of colour (hypoxia) Cluster of DNA remnants in RBC (splenectomy) RBC composed of denatured haemoglobin Cells combine ```
30
``` Staining Romanowsky stains Made from? Study? Contains? Used for? ```
Water soluble eosin, methylene blue, absolute methanol Malaria parasite Leishmans (ppe) , diff quick(fixation solution methanol (blue), stain solution 1 eosin (red), stain solution 2 methylene blue (purple)) , giemsa Differential white cell count, presence of blood parasite, rough platelet count
31
``` Staining Supra-vital sign Introduced into? Includes? Used for? ```
Living tissue removed from body but begot cessation of chemical life Methylene blue, brilliant cresyl blue Reticulocyte count Detect Heinz body
32
``` White blood cells Difference? Neutrophil Basophil Eosinophil Monocyte Lymphocyte Increase in band neutrophil = Manual counting uses ```
``` G. Size and staining G. Inflammation from infection G. Increase with Allergic reaction AG. Increase with parasitic infection AG. Acquired immunity A shift to the left Diluting chamber and haemocytometer ```
33
``` Packed cell volume Measures? Anticoagulant? Dog Cat Horse Myeloid = ```
``` Hydration and blood loss EDTA 37-55% 24-45% 30-49% Bone marrow ```
34
AST test | BUN
Liver function | Kidneys (blood urea nitrogen)
35
``` Urine Pigment? Green? Sg measures? Increase? Decrease? Ph dog, cat, horse? Haemoglobinuria Myoglobinurea Centrifuge ```
``` Urochrome Biliverdin/meds Density of known volume of fluid Renal, sediment, fluid loss, chemical Hyperthyroidism, pu/pd, renal, steroids 5-7, 7-9, 7-9 Iv haemolysis Trauma to muscles 1,500 RMP, 5 minutes ```
36
``` Casts Formed? Consist of? Form in distal tubules if? Dissolve in? Types? ```
``` Kidney tubules Precipitated proteins Acidic conditions Alkaline urine Hyaline (rectangular, clear) Cellular (clump of cells) Granular (grainy) Waxy (hyaline but square, not clear) ```
37
``` Crystals Triple phosphate/magnesium ammonium phosphate/struvite Ca phosphate Ca oxalate Ammonium biurate Uric acid Cystine Calcium carbonate Leucine Tyrosine Phosphate and calcium carbonates = ```
``` Alkaline, bacterial UTI Alkaline urine Acidic, antifreeze Acidic, dalmations, pss Acidic Acidic Alkaline, Equine and rabbit Acidic Acidic Alkaline ```
38
``` Vernier Invented by? Record along? Values? Whole number then a? Demodex Otodectes Cheyletiella ```
``` Pierre vernier X, Y axis 11 values Fraction Hair pluck Hair pluck Tape strip ```
39
Floatation = | Baerman technique detects?
``` Mcmasters technique (strongyle and parascaris equorum) salt, sugar, sodium nitrate, magnesium or zinc sulphate, 1500, 2 minutes, 3g, 42ml Angiostronguluas, 20g, 24hrs, iodine to highlight ```
40
``` Microbiological media Carbon compounds = Nitrogen compounds = Simple media contains? Enriched media Blood agar contains? Chocolate agar? Selective media? Selenite broth Mcconkeys agar Desoxychocolate citrate agar Biochemical media incubates? Plate storage at? Glassware cleaned with? Incubate plates at and for? ```
``` Energy Proteins for growth Basic growth factor (broth and agar) 10% animal blood (B-haemolytic streps) Heated (80°) rupture RBC Salmonella Ferment lactose Salmonella Dermatophytosis (destroy in autoclave, 121) 5°c Soft bristled brush 37°c and 18-24 hours ```
41
``` Staining slide Methylene blue stained for? Grams method? + - Primary stain = Mordent Decolouriser Red stain added to counter stain Ziehl-neilsens method Detect? ```
Blue for 2 minutes Purple - increase peptidoglycan, no lipopolysaccarhide Pink - botj peptidoglycan and lipopolysaccarhide Violet (methyl, crystal, gentain violet) Fixes violet stain (lugols iodine, gram iodine) Acetone Acidic fast organisms (Tuberculosis) Acidic fast =red, non = blue
42
``` Sensitivity testing Pg S Ot Ap C Ts Cfx A ```
``` Penicillin g Streptomycin Oxytetracycline. Amipenicillin Chloramphenicol Cotrimoxazole Cephalexin Amoxycillin ```
43
Fungal cultures | Selective media
Sabourauds agar | Yellow to red