Lab Topics

Question 1

What are the main parts of a compound light microscope?

Answer 1

• Ocular lens
• Diopter adjustment
• Head
• Arm
• Nose piece
• Objective lenses
• Stage clip
• Aperture
• Diaphragm
• Condenser
• Illuminator
• Mechanical Stage
• Fine adjustment
• Coarse adjustment
• Stage controls
• Brightness adjustment
• Light Switch

Question 2

How to convert microliters to militers?

Answer 2

Divide by 1000

Question 3

What is the mean, standard error, and standard deviation formulas used for Excel?

Answer 3

Mean: =AVERAGE
Stand. Dev.: = Stdev
Stand. Error: =SD/SQRT(Sample Size)

Question 4

What would be considered room temperature?

Answer 4

22 degrees celsius

Question 5

What is a dataset?

Answer 5

It is the total set of information that derive from multiple sources.

Question 6

What are the different types of data?

Answer 6

Quantitative (Discrete and Continuous) and Qualitative

Question 7

What is the scientific method?

Answer 7

A systematic framework that provides biologists and other modern scientists a way to help logically organize the progression of their scientific studies.

Question 8

What is confirmation bias?

Answer 8

This tendency to search for, interpret,

favor, and recall information in a way that confirms one’s preexisting beliefs or hypotheses

Question 9

What is the correct order of the scientific method?

Answer 9

Observation, question, hypothesis, prediction, experiment, conclusion

Question 10

What are dependent variables? (y-axis)

Answer 10

factors that are actually measured, counted, or observed. They
are the variables you expect to change in response to the independent variable.

Question 11

What are independent variables? (x-axis)

Answer 11

are factors that are intentionally manipulated in an experiment
because it is predicted that they will affect the dependent variable.

Question 12

What are controlled variables?

Answer 12

are factors/conditions held constant between control and treatment
groups.

Question 13

Experiments can prove that hypothesis are true.

Answer 13

False! They cannot prove anything, but only provide support for the analysis.

Question 14

What is a prediction?

Answer 14

If, then statement

Question 15

What is the temperature coefficient formula?

Answer 15

Q10 = [rate at (ToC +10)] / [rate at ToC]

Question 16

Which color is the 4x microscope lenses?

Answer 16

Red

Question 17

What is the instrument used in lab to measure liquids?

Answer 17

Micropipette. The one we use in lab is called P1000 because it can dispense a maximum of 1000 microliters, which is equal to 1 mililiter.

Question 18

If we see 0500 on the micropipette display, it means that we…

Answer 18

Have 500 microliter and 0.5 ml

Question 19

What is a spectrophotometer?

Answer 19

It is a machine that analyzes the wavelength of colored substances.

Question 20

What is a cuvette?

Answer 20

A rectangular container used in the spectrophotometer.

Question 21

What is the formula for enzyme activity?

Answer 21

Absorbance/(1.36^10-4) * T

Question 22

Define the range for an acidic, a basic, and a neutral pH.

Answer 22

Acidic: <7
Neutral: 7
Basic: >7

Question 23

What is the meaning of the overlapping error bars?

Answer 23

They mean that there is no significant difference between the groups.

Question 24

What is the main goal of a pie chart?

Answer 24

To represent sample proportions

Question 25

What is the main goal of a bar or column graph?

Answer 25

It represents the frequency of each species as a column.

Question 26

What is the main goal of a histogram?

Answer 26

To represent the frequency of continuum data in intervals provided by us.

Question 27

What is the main goal of a scatterplot?

Answer 27

To connect data represented by points (x,y)

Question 28

The null effect is said to

Answer 28

Predict no effect

Question 29

What are the three main groups we should see in a study?

Answer 29

Test or treatment group; negative control group; positive control group.

Question 30

What is modern systematics?

Answer 30

The study of the diversification of living forms.

Question 31

What is the main goal of ANOVA and T-test?

Answer 31

They are used to determine is there is a significant difference between the means of two or more groups by examining within group and between group variations in the data.

Question 32

If there are two groups being compared then we should use…

Answer 32

a T-test

Question 33

If there are three or more groups being compared…

Answer 33

We should use one-way ANOVA

Question 34

When we run statistics through Excel, what are the data we should focus on?

Answer 34

Mean, variance, and p-value.

Question 35

What is the most common p-value used by scientists?

Answer 35

P-value = 0.05

Question 36

If the p-value exceeds 0.05….

Answer 36

we will accept the null hypothesis

Question 37

If the p-value is lower than 0.05…

Answer 37

we reject the null hypothesis in favor of the alternative hypothesis

Question 38

What is a hemocytometer and how should we use it?

Answer 38

An improved Neubaurer Hemocytometer is a microscopic slide with grids to help with identifying and counting specimens.

Question 39

What is the total volume for one large corner of the square in a hemocytometer?

Answer 39

0.1 microliters

Question 40

How to measure the respiration rate of an organism?

Answer 40

By measuring its uptake of oxygen or production or carbon dioxide.

Question 41

What is the gas-phase CO2 sensor used in one of our labs?

Answer 41

Respirometer

Question 42

What does ppm mean?

Answer 42

Parts per million

Question 43

What is the formula for a linear equation?

Answer 43

y=mx+b