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BIOL-111-FRO1 lab > LAB TEST > Flashcards

LAB TEST Flashcards

1
Q

NaOH is the abbreviation for

A

Sodium Hydroxide

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2
Q

The red triangle (if on a chemical label of an actual container) will contain a number that refers to

A

Flammability

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3
Q

How many more H+ ions per Liter will there be in a solution go pH 4 compared to pH6

A

100

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4
Q

How much mass of potassium permanganate is contained

A

0.10 kg

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5
Q

When mixing cane sugar with water, the sugar disappears because it

A

is soluble

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6
Q

How many more H+ ions per liter will there be in a solution of pH3 compared to pH6

A

1000

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7
Q

Buffers are important chemicals that keep a stable ____ in organisms

A

pH

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8
Q

The yellow triangle (if on a chemical label of an actual container ) will contain a number that refers to

A

Instability reactivity

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9
Q

How many more H+ ions per liter will there be in a solution of pH 3 compared to pH 7

A

10,000

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10
Q

True or False Enzymes can be considered activists which bring molecules together

A

False

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11
Q

Your stomach has a pH of 2. Its contents are

A

Acidic

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12
Q

When enzymes lose their 3d shape they lose the ability to function. this change of shape that leads to loss of function is called

A

Denaturation

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13
Q

True or false
Catalase is found only in animal cells

A

False

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14
Q

Catalase break down which toxic substance in cells

A

Hydrogen Peroxide

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15
Q

Which of the following are the products of the reaction catalyzed by catalase

A

water and oxygen

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15
Q

in the catalase experiment, what do bubbles represent

A

Breakdown of hydrogen peroxide into oxygen gas

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16
Q

after boiling the test tubes, what is the next step before testing for catalase activity

A

allow the test tubes to cool

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17
Q

About how much hydrogen peroxide should be added to each test tube when testing for catalase activity

A

5ml

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18
Q

why is it so important to boil some of the tissues (liver or potato) in this experiment

A

to observe how cooking changes the tissue color

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19
Q

what type of microscope will be used In this lab

A

Compound light microscope

20
Q

What is the total magnification when using a 10x ocular lens and a 40x objective lens?

A

400x

21
Q

What does the diaphragm lever control?

A

The light passing through the specimen

22
Q

Why should you avoid using the coarse focus knob when the high-power objective is in place?

A

It could hit (and maybe break) the slide or lens

23
Q

When increasing the magnification, what typically happens to the field of view?

A

It decreases

24
Q

What will happen to the resolution of the image when the magnification is increased?

A

Resolution will increase

25
Q

Which term describes the range of depth that can be in focus at one time?

A

Depth of field

26
Q

What should you first try if you have turned the coarse adjustment knob all of the way and the image is not in focus?

A

Return the coarse focus knob to the starting position and try again slowly

27
Q

How should you carry the microscope?

A

With both hands: one on the arm and one on the base

28
Q

Which of the following lenses provides the smallest depth of field?

A

High-power objective

29
Q

What is the main purpose of preparing a bacterial smear?

A

To adhere the cells to the slide so they are not washed off during staining

30
Q

Why is it important to prepare a thin smear?

A

To visualize individual cells and their arrangement

31
Q

What is the primary goal of heat-fixing the smear?

A

To kill and adhere the bacteria to the slide

32
Q

What is a potential consequence of using too much heat when fixing a slide?

A

The slide could shatter.

33
Q

How should the inoculating loop be treated before touching the bacterial culture?

A

Flame the loop until it is red-hot and allow it to cool

34
Q

What is the main purpose of the Gram staining technique?

A

To differentiate bacteria into two groups based on cell wall composition

35
Q

Gram-negative bacteria appear what color after Gram staining?

A

Red/Pink

35
Q

What is the primary difference between Gram-positive and Gram-negative bacteria in terms of cell wall structure?

A

Gram-positive bacteria have a thicker peptidoglycan layer

36
Q

Gram-positive bacteria appear what color after staining?

A

purple

37
Q

In the Gram staining process, what happens if you skip the iodine step?

A

Both types of bacteria will appear red

38
Q

What shape is DNA?

A

Double helix

39
Q

What are the three components of a nucleotide?

A

Base, sugar, and phosphate group

40
Q

What is DNA extraction?

A

The process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered.

41
Q

True or False: Never lay a pipette with a sample in it on its side. Chamber contamination can result.

A

True

42
Q

Which precipitation method is used during this experiment?

A

Salt precipitation

42
Q

Fill in the blank: ______ will be the basis of almost all the experimental work that you’ll do. So it is important to develop good technique in order to get precise and accurate results.

A

Pipetting

43
Q

What does the sequence of bases in DNA determine?

A

The information available for building and maintaining an organism

44
Q

How should you dispense the sample out of your micropipette?

A

Depress the push button to the first stop, and then depress to the second stop

45
Q
A

BIOL-111-FRO1 lab (1 decks)

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