Lab Techniques Flashcards
Suggest two precautions which should be put in place when working with chemicals
Ensure pregnant workers do not use it Wear gloves Wear a lab coat Have an established procedure for spills Maintain a clean working area before and after use Ensure they are disposed of safely
What is PPE
personal protective equipment
Why is PPE used
To protect the body from harm
Give an example of PPE which is appropriate for working with UV light
A lab coat
Safety glasses
A face shield
Why are buffers used in an experiment
The keep the ph constant
Name a piece of equipment used to measure the absorbance of a solution
Colorimeter
What type of cells produce antibodies
B lymphocytes
What term is used to describe fused cells
Hybridomas
Name the chemical used to fuse cells together
PEG polyethylene glycol
Other than a microscope, what piece of equipment is required to perform a cell count
Haemocytometer
Name a biological control measure
Use a more sustainable strain of micro organism
i.e. Less virulent
Introducing genes to the organisms which prevent it from surviving outside the lab
What are autopipettes used for
Measuring small volumes precisely
Explain how autopippettes are used
Like a syringe but have two stops
1st for drawing
2nd for dispensing
What are curettes used for
To dispense precise volumes of liquid reagents i.e. For titration
What is a dilution series
Repeated dilution from a stock solution
