Lab Skills and Calculations

Question 1

Calculations for solutions using mass/volume concentration equation.

Answer 1

c x v = mass

Question 2

Calculation for solutions based on percentage.

Answer 2

% as g/ml x volume = mass of solute (g)

Question 3

What is the molarity concentration equation?

Answer 3

Volume (L) x molarity desired (mol/L) x MW (g/mol) = solute (g)

Question 4

What are the four main pipettes?

Answer 4

P10, 20, 200 and 1000

Question 5

What is the SNP we used and the region it amplifies?

Answer 5

SNP785 and 312bp.

Question 6

What does the lysis buffer contain and why?

Answer 6

Detergent - break cell membrane.
Chelating agent (EDTA) - prevents DNAses from breaking DNA

Question 7

What is the desired A260/A280 ratio and what does it indicate if it is less?

Answer 7

1.8 - 2.0
Contamination has occurred if less.

Question 8

How is DNA concentration calculated?

Answer 8

In ng/ul
A260 x 50ng/ul x dilution factor

Question 9

What is the specific restriction enzyme and its sequence?

Answer 9

FnuH1 - GCNGC/CGNCG region

Question 10

Why must the restriction enzyme be kept on ice?

Answer 10

It is thermolabile.