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LAB SAFETY AND QUALITY CONTROL MEASURES Flashcards

1
Q

Hazard identification
Risk assessment and control
Biosafety and biosecurity measures
Safety skills, knowledge and training

A

Elements of laboratory safety

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2
Q

Use a Biosafety Level 2 (BSL2) facility
Separate laboratory for fungal and viral soecimens
Use a Class II biosafety cabinet
Ensure proper monitoring and maintenance

A

Fungal Specimen processing

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3
Q

TRUE OR FALSE

Fungal infection and contamination is more complicated to handle than bacterial agents

A

TRUE

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4
Q

TRUE OR FALSE

Do not always wear PPE during specimen processing

A

FALSE

always wear PPE during specimen processing

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5
Q

When handling clinical isolates especially dimorphic pathogens what position do we maintain it so that it prevents aerosolization

A

Maintain on slants

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6
Q

Plated media Handling

Seal with ___ before removing from the biosafety cabinet

A

gas-permeable tape

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7
Q

Culture vessels for fungal culture must be sealed because of ___

A

spores

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8
Q

Surface decontamination

Use __% bleach for surfaces in contact with clinical specimens or culture

A

10%

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9
Q

Dispose of clinical materials

Use ___ bags secured with tape and dispose in designated biomedical waste containers

A

biohazard

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10
Q

Personne in the Mycology laboratory should be

A

Healthy
Not on antibiotics or immunosuppresive drugs
Trained on safety techniques

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11
Q

Biological Safety Cabinet (BSC)
Alcoholic sand flask
Plastic-baked gauze/cotton pads
Stainless steel containers

A

Essential equipment and materials

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12
Q

Mycology laboratory

Prohibited activities

A

smoking, eating

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13
Q

Mycology laboratory

Handwashing protocols

A

Soap and water; disinfectant solution

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14
Q

Mycology laboratory

Use of biological safety cabinets

A

BSC Class IIA

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15
Q

Mycology laboratory

Transport of cultures and specimens

A

racks or trays

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16
Q

Mycology laboratory

Aerosol reductions

A

electric incinerators minimize aerosol and heat output

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17
Q

Mycology laboratory

Handling contaminated liquids

A

splash-proof containers; avoid aerosols

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18
Q

BSL 2 work practices and facilities
Use of secondary containers for specimen transport
Specimen processing in Class II biosafety cabinets
Disinfection with bleach and alcohol

A

Virology Laboratory Safety considerations

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19
Q

Boiling
Pasteurization
UV Light irradiation

A

Physical Methods of disinfection

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20
Q

Not less than 62.8°C or more than 65.6°C for at least 30 min (LTLT; holder method)

Not less than 71.7°C for at least 15 s (HTST)

A

Pasteurization

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21
Q

At 100°C for 15 minutes

A

Boiling

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22
Q

Not recommended for disinfection of biosafety cabinets because of ineffective penetration; __ deteriorates plastic in the BSC; ___ light intensity decreases over time

A

UV light irradiation

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23
Q

Ethanol (60%-80%)
Ethanol (70%)
Phenolic compoinds
Quaternary ammonium compounds
Chlorine bleach
Chlorine bleach followed by rinsing with 70% alcohol or water

A

Chemical methods of disinfection

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24
Q

Active against enveloped viruses (Herpesviruses, vaccinia virus, influenza virus) and non-enveloped viruses (adenovirus, enterovirus, rhinovirus and rotaviruses) ; also effective against bacteria, fungi, and mycobacteria

A

Ethanol (60%-80%)

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25
Q

Disinfects external surfaces of equipment and small surfaces

A

Ethanol (70%)

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26
Q

Bactericidal, fungicidal, tuberculocidal, and virucidal but not effective against enterovirus

A

Phenolic compounds

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27
Q

Fungicidal, bactericidal and virucidal (only against enveloped viruses); not sporicidal and tuberculocidal

A

Quaternary ammonium compounds

28
Q

Recommended for surface disinfection and has a broad spectrum of activity; used for decontamination of blood spills (1:10 dilution of 5.15% to 6.25% sodium hypochlorite)

A

Chlorine bleach

29
Q

Recommended to decontaminate stainless steel surfaces

A

Chlorine bleach followed by rinsing with 70% alcohol or water

30
Q

Moist heat (steam)
Incineration
Flame sterilization
Dry heat (hot air)

A

Physical methods of sterilization

31
Q

Ethylene oxide

A

Chemical Methods of Sterilization

32
Q

Sterilization of supplies and media (121°C for 15 minuets) and medical wastes (132°C for 30 to 60 minutes)

A

Moist heat (steam)

33
Q

Medical wastes

A

Incineration

34
Q

Used for metal tools including inoculating loops and wires, tips of forceps, glass objects such as culture tubes and microscope sludes

A

Flame sterilization

35
Q

For sterilization of dry glassware including flasks, pipettes, tubes, petri dishes, and metal instruments

A

Dry heat (hot air)

36
Q

Most common chemical method

A

Ethylene oxide

37
Q

Recommended when working with microbes not known to cause disease in healthy adults (e.g Saccharomyces cerevisae)

38
Q

Access is restricted to authorized personnel
Readily accessible sinks for hand washing
Readily accessible eyewash stations
Wearing of appropriate PPE
Laboratory bench tops must be resistant to chemicals and liquids
Laboratory surfaces and equipment are easily cleaned and disinfected
Decontamination of solid wastes

39
Q

Recommended for microbes associated with human disease but are not aerosol-transmitted (e.g Candida ablicans, Cryptococcus neoformans, Hepatitis B virus, Herepes simplex virus)

40
Q

Universal biohazard signs
Processing of specimens in a BSC
Decontamination of equipment must be available (e.g autoclave)
Use of appropriate PPE
Sharps are placed in a puncture-resistant containers

A

BSL 2

(+ BSL 1 practices)

41
Q

Recommended for hazardous microbes that are primarily aerosol-transmitted (e.g Blastomyces dermatitidis, West nile virus)

42
Q

Controlled access to the laboratory
Cultures and clinical materials are processed in a BSC (Class II)
Negative-pressure airflow
HEPA-Filtered exhaust air from BSCs
Use of appropriate PPE and containment devices
HEPA-filtered respirators or masks
Serologic determination of immune status of personnel

A

BSL 3

(+ BSL 1 and 2 practices)

43
Q

Recommended for agents that can cause life-threatening or untreatable diseases brought about by aerosols or unknown transmission (Ebola virus, Marburg virus, Crimean-congo hemorrhagic fever virus)

44
Q

Change of clothing before entering, and shower on exit
Decontamination of all wastes of exit
Use of BSC (Class III)
Specialized ventilation and waste management systems

A

BSL 4

(+ BSL 3 practices)

45
Q

Goal of Quality Control

A

Accuracy
Reliability
Reproducability

46
Q

Closeness to actual results

47
Q

Standard of quality over time

A

Reliability

48
Q

Consistency of measurement
-Different appraisers
-Same equipment

A

Reproducibility

49
Q

Check for cracks, uneven filling, hemolysis, freezing, drying out, bubbles,contamination

A

Inspection of media and petri dishes

50
Q

Verify media are within expiration dates

A

Expiration dates

51
Q

Check for contamination or foreign substances

A

Reagent inspection

52
Q

Record temperatures of refrigerators, freezers, and incubators
Acceptable variation:±1°C
Include humidity and CO2 readings if applicable

A

Daily temperature monitoring

53
Q

Avoid placing objects on grid panels to maintain airflow
(Perform smoke testing before use)

A

Biological safety cabinets

54
Q

Use distilled or deionized water
Maintain temperature fluctuation within ±1°C

A

Water baths

55
Q

Follow manufacturer’s instructions or package insert strictly

A

Test kit storage

56
Q

Waste disposal Chemical wastes :Treatment methods

A

Decomposition, decontamination, landfill disposal

57
Q

Waste disposal Chemical wastes

Flammable fluids and chemicals

A

Original, securely sealed, and labeled containers
Never pour down the drain

58
Q

Waste disposal Chemical wastes

Risks of improper disposal

A

Hazardous fumes
Disruption of sewage treatment facilities
Damage to laboratory plumbing

59
Q

Waste disposal Chemical wastes

Disposal of non hazardous solvents : Water soluble and less than a pint

A

flush with plenty of water

60
Q

Waste disposal Chemical wastes

Disposal of non-hazardous solvents : For non-soluble or volumes over one pint

A

use a designated solvent waste container

61
Q

Waste is labeled, segregated by compatibility, stored in secondary containment, and in an isolated area

A

Proper hazardous waste storage

62
Q

No VEHS labels
No secondary containment
No segregation and containers covered in residue

A

Improper hazardous waste storage

63
Q

Waste disposal Solid wastes

Risk of puncture wounds and infectious agents
Use leakproof, puncture-proof containers labeled as contaminated waste

A

Needles, syringe, and scalpels

64
Q

Waste disposal Solid wastes

Dispose in labeled cardboxes, not reular trash cans

A

Broken and damaged glassware

65
Q

Waste disposal Solid wastes

Paper towels, packaging materials, certain plastics
Discard in regular trash bins

A

Non-contaminated waste

66
Q

Waste disposal Solid wastes

Highest risk level in medical mycology laboratory: includes clinical specimens, contaminated materials, cultures

A

Biologically contaminated waste

67
Q

Waste disposal Solid wastes

Use discard pans with 5% hypochlorite solution or similar disinfectant

A

Biologically contaminated waste

MYVCOVIRO LAB (2 decks)

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