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MBIO 1010 Lab > Lab Safety > Flashcards

Lab Safety Flashcards

1
Q

When do you disinfect your lab area?

A

before and after each lab session

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2
Q

How do you disinfect the lab work area?

A

wet the counter with disinfectant and wipe over surface with paper towel

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3
Q

When should you wash your hands?

A

before leaving lab

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4
Q

How long should you wash your hands?

A

for at least 15 seconds

with soap and water

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5
Q

How do you avoid inadvertent contamination to your mouth?

A

keep fingers and pencils away from it

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6
Q

Can you eat and drink in the lab?

A

no

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7
Q

What do you do if equipment is missing or not working properly (especially microscopes)?

A

report to the instructor immediately

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8
Q

Are you allowed personal effects in the lab?

A

no

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9
Q

What is the lab coat policy?

A

must wear a buttoned lab coat at all times

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10
Q

How do you clean your lab coat between labs?

A
  • take home in plastic bag, do not let come into contact with other belongings
  • wash separately from other clothes with detergent and bleach
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11
Q

Can your lab coat be worn in other labs before it is washed?

A

no, it must be washed first

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12
Q

What do you do if you know/suspect your lab coat has been contaminated with bacterial culture?

A

report incident to demonstrator

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13
Q

What happens when the demonstrator determines the appropriate means of decontaminations for a contaminated lab coat?

A

spot disinfection or sterilization in the autoclave

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14
Q

What happens if sterilization int he autoclave is needed for a contaminated lab coat?

A

lab coat remains overnight in the lab and returned to you the following day

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15
Q

What does risk group 1 cultures mean?

A

unlikely to cause disease in healthy workers

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16
Q

What does risk group 2 cultures mean?

A

can cause human disease but are unlikely to under normal circumstances (immune compromised may be at risk)

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17
Q

How should you treat all organisms in the lab?

A

like they cause disease

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18
Q

What are the safety measures when handling risk group 2 organisms?

A
  • wear disposable gloves

- be aware of aerosols when flaming loops

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19
Q

Where is paper waste discarded?

A

regular waste paper baskets

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20
Q

Where is used petri plates discarded?

A

large biohazard bins sitting beside door

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21
Q

Where is disposable gloves discarded?

A

large biohazard bins sitting beside door

22
Q

Where is glass slides, broken glass and Pasteur pipettes discarded?

A

broken glass/sharps discard containers

23
Q

Where is used swabs and toothpicks discarded?

A

broken glass/sharps containers

24
Q

Where is disposable pipettes discarded?

A

broken glass/sharps containers

25
Q

What do you do with used glassware (test tubes, beakers, bottles and flasks)?

A
  • clean of all labels

- place upright in the trays on the glassware discard trolleys

26
Q

Do you pour stuff down the sink in the MBIO lab?

A

NO

27
Q

What do you do for a spilled bacterial culture when broken glass is involved?

A

notify instructor or demonstrator immediately

  • do not pick up glass with fingers
  • prevent other students from walking in it
  • instructor cleans up
28
Q

What do you do for a spilled bacterial culture if no broken glass is involved? (5 steps)

A
  • cover spill with paper towel
  • squeeze disinfectant onto outer edges of spill and work towards centre
  • allow to sit for at least 1 minute
  • gather up soaked towels (throw in regular garbage)
  • disinfect area again with fresh paper towels and discard in regular garbage
29
Q

Why do you squeeze disinfectant onto outer edges of spill and work towards centre on a bacterial culture spill?

A

minimizes amount of aerosols

30
Q

What do you do if culture is splashed on skin?

A

wash skin with alcohol (handwashing sink), then wash with soap and water

31
Q

What do you do if culture is splashed into eyes?

A

rinse eyes with water from nearest tap/eyewash station

-avoid rubbing eyes

32
Q

What do you do for accidental contamination of the mouth?

A

treated by rinsing with copious amounts of water and reported to the instructor

33
Q

What do you do for cuts or wounds not he hands or fingers?

A

should be kept covered by bandages, and the bandage should be covered with a glove
-see TA if you need a bandaid

34
Q

What do you do for clothes that have been contaminated?

A
  • disinfected with small amount of disinfectant (from washing sinks) and then allow to dry completely
  • once dried, contaminated clothes should be washed separately with detergent and bleach
35
Q

What do you do if a fire occurs?

A

turn off gas and call for lab instructor

36
Q

What must be done to hair in the lab?

A
  • long hair tied back

- excessive use of hairspray and other products should be avoided

37
Q

How do you light a Bunsen burner?

A

with a striker provided

38
Q

What to do when clothing is on fire?

A

smother it

39
Q

What do you do in case of a fire alarm?

A

wait for signal from instructor to evacuate lab, head for stairs and exit

40
Q

Do you use the pilot flame of the Bunsen burner for sterilization?

A

NO

41
Q

What does WHMIS stand for?

A

Workplace Hazardous Materials Information System

42
Q

What is WHMIS?

A

a system for safe management of hazardous materials

43
Q

Who legislates WHMIS?

A

provincial and federal governments

44
Q

What are the 3 parts of WHMIS?

A

cautionary labels, safety dada sheets (SDS) and worker education programs

45
Q

What does SDS stand for?

A

safety data sheets

46
Q

What are cautionary labels?

A

placed on all containers with controlled products

47
Q

What 2 places must cautionary labels be placed on?

A

containers obtained by suppliers and those dispensed in the workplace

48
Q

What 7 parts must be included on the supplier labels?

A
  • product identifier
  • supplier identifier
  • SDS
  • hazard symbol
  • risk phrases (nature of hazards)
  • precautionary measures
  • first aid measures
49
Q

What are the 3 parts of workplace labels?

A
  • product name
  • precautionary measures (simplified)
  • reference to availability of SDS
50
Q

What does MSDS stand for?

A

material safety data sheets

51
Q

What is MSDS

A

links to online SDS for controlled products and biohazardous materials available on installed laboratory computers

MBIO 1010 Lab (1 decks)

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