lab safety

Question 1

Name 5 BSL 3 organisms

Answer 1

Rickettsia, Brucella, HIV, Coxiella burnetti, Western equine encephalomyelitis virus

Question 2

Purpose of quality assurance in lab

Answer 2

reliability, accuracy, reproducibility

Question 3

Time frame for a lab to notify state if moving or changing a name

Answer 3

30d

Question 4

OSHA requires an exposure plan when a lab has

Answer 4

biohazardous materials

Question 5

QC methods performed with every batch of media prepared

Answer 5

sterility check

Question 6

Diseases reported to local health officer

Answer 6

anthrax, avian influenza, botulism, brucellosis, burkholderia, plauge, smallpox, tularemia, hemorrhagic fever (ebola/marburg)
Annie always brought brian burgandy pants smaller than him.

Question 7

a clinical lab must notify the health officer for cases of

Answer 7

botulism

Question 8

Name the organization that regulates labeling and shipment of etiologic agents

Answer 8

Interstate Quarantine Regulations (governed by Code of Federal Reg-Dept of Transportation)

Question 9

glassware sterilization procedure

Answer 9

autoclave at 15lbs pressure, 220^C for 15min

Question 10

service records included in equipment performance and maintenance logs

Answer 10

calibration
validation
maintenance

Question 11

water supply equipment should check for

Answer 11

conductivity

Question 12

4 requirements for submission to MDL of specimens

Answer 12

1. use correct mailing containers
2. provide all information
3. collect adequate voluems (10 blood, 3 serum, 3 CSF)
4. submit through MDL

Question 13

medical labs carry records for how many years? medicare?

Answer 13

medical: 3 y

medicare 2y

Question 14

Purpose of QC systems

Answer 14

1. reliability, accuracy, reproducibilty

2. meet established standards

Question 15

primary and secondary objectives of proficiency testing

Answer 15

1. provide labs with performance quality

2. provide physicians assurance of quality

Question 16

5 aspects of quality control program

Answer 16

1. SOPs
2. Media prep manual
3. equipment monitoring/logs
4. preventive maintenance
5. CAPs subscription
6. QC surveillance reports

Question 17

most important QC legally-required record

Answer 17

up-to-date lab procedure manual

Question 18

define sterilize

Answer 18

physical/chemical procedure to destory microbial life and spores

Question 19

define disinfectant

Answer 19

phys/chem procedure to elminate pathogenic microorganisms, but not al microbial forms

Question 20

antisepsis

Answer 20

germicide used on skin to inhibit surivial of microorganisms

Question 21

most effective method of sterilization

Answer 21

steam

Question 22

personal contamination procedure

Answer 22

1. alert
2. clean exposed surface
3. apply first aid
4. notify supervisor
5. report to medical clinic

Question 23

Surface contamination procedure

Answer 23

1. alert
2. isolate contaminatearea
3. ppe
4. cover spill
5. apply disinfectant 20 min
6. clean up spill, dispose materal
7. notify supervisor

Question 24

examples of disinfectants

Answer 24

chlorine, paraformaldehydes, phenols, ammonium compounds

Question 25

examples of antiseptics

Answer 25

70% alcohol, hand soap, iodine compounds

Question 26

lab director responsibilities that can be delegated to supervisor

Answer 26

1. remedial actions that deviate from SOPs
2. patient test results not reported until test functions properly
3. orientation provided to all personnel
4. evals and CAP documentation