Lab quiz Flashcards

1
Q

Why do tissues able to stain?

A

because of van der Waals’s forces

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2
Q

Who developped first microscope ?

A

Antonie van Leeuwenhoek

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3
Q

(TF) did the first microscope used below light?

A

no because used metal plate so examinated with natural light (or candlelight)

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4
Q

(TF) if a use the 20x objective lens I am looking at the 20x magnification.

A

False
Ocular lenses magnify 10x so total magnification is 10 x magnification of objective lens (eg 10x20=200x)

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5
Q

What can we use to control light source on a compound microscope?

A

To control light: diaphragm and condenser

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6
Q

What are the 4 types of tissues?

A

epithelial tissue
connective tissue
muscle tissue
nervous tissue

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7
Q

What are the steps in paraffin sectioning?

A
  1. sample collection ( appropriate thickness )
  2. fixation (buffered formalin, formaldehyde for hardening)
  3. dehydration (increase alcohol concentration to displace water out tissues)
  4. clearing (remove alcohol with xylol)
  5. infiltration ( drop tissues into warm liquid paraffin wax (get solid at RT). Paraffin allow smooth sectioning)
  6. sectioning (w/ microtome or blade)
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8
Q

What are the steps in staining paraffin section?

A
  1. remove wax with xylol
  2. hydration (add water to decrease alcohol concentration)
  3. staining (water-based stain)
  4. dehydration (increase alcohol concentration)
  5. clearing (xylol remove alcohol and extra stain)
  6. mounting (Permenant when between 2 glasses)
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9
Q

Explain most common dyes in histology, which cell is stained.

A

H&E use 2 stains: hematoxylin need mordant (alum) to stain nucleic acids, nuclei, RER -> blue to black
Eosin stain cytoplasm -> pink, orange,red

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10
Q

What can affect the color of tissues staining ?

A

Color Depend on: size, material, time exposure, concentration, how long wasted off, size nucleus, genetic material, nucleic acid content

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11
Q
A
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