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BIO 220 - [Lab Quizzes] > Lab Quiz #1 - Microscope Quiz > Flashcards

Lab Quiz #1 - Microscope Quiz Flashcards

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1
Q

What is the definition of a compound microscope?

A

instrument consisted of at LEAST 2 lenses: ocular lens (eyepiece) and objective lens

(our microscopes also have a condenser lens)

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2
Q

What is the function of the compound microscope?

A

magnify the image; NOT the object

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3
Q

What is the definition of resolution?

A

ability of microscope to show fine structure details with clarity/sharpness

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4
Q

What is the function of the main/power switch?

Know where this is located

A

turns on light source

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5
Q

What is the function of the brightness adjustment knob?

Know where this is located

A

regulates amount of light to the microscope

(set it to comfortable eye setting)

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6
Q

What is the function of the light source/LED bulb

Know where this is located

A

provides white light

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7
Q

What parts of the microscope can you find at the base?

A

main/power switch j
brightness adjustment knob
(light source/LED bulb)

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8
Q

What is the substage assembly area?

A

area underneath the stage

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9
Q

What parts of the microscope can you find in the substage assembly area?

A

iris/aperture diaphragm with lever

condenser lens

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10
Q

What is the function of the iris/aperture diagram with lever

Know where this can be found

A

fine tunes the light

(slowly move lever to let light in)

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11
Q

What is the function of the condenser lens

Know where this can be found

A

prevents scattering of light as it travels from the bottom

seen through hole in stage and under specimen

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12
Q

Name all of the compound lenses in our compound microscope

Which lens does NOT contribute to magnification?

A

ocular lens
objective lens
condenser lens

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13
Q

Compare Bright Field Setting/Microscopy vs. Dark Field Microscopy: backgound; object color; light; specimens

A

BF setting/microscopy
- light background
- colored/dyed object
- light passes through specimen
- specimen is dead

DF setting/microscopy
- dark background
- object NOT colored; no dyes used
- light reflects off specimen = shiny appearance
- used for live specimens

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14
Q

Why is BF setting/microscopy used to observe dead specimens?

A

In BF setting/microscopy, we use it to observe colored/dyed specimens. dyes are toxins that will kill things, therefore, any specimen that is dyed has literally dieded 😂

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15
Q

What is the function of the stage?

Know where this is located

A

rest the slide/specimen on top of it

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16
Q

What is the function of the slide adjuster knob?

Know where this is located

A

moves slide back and forth and side to side to scan the slide starting at the lowest magnification (4x)

  • part of stage -
17
Q

What is the function of the scale of specimen position?

Know where this is located

A

are coordinates for positioning/pinpointing a landmark on the slide

  • part of stage-
18
Q

TRUE or FALSE: the scale of specimen position is used for measuring size of specimen

A

False, scale of specimen are coordinates for positioning/pinpointing a landmark on slide

19
Q

TRUE or FALSE: Objective lenses have a constant magnification

A

False, objective lenses vary in magnification. Ocular lens have a constant magnification of 10x

20
Q

Which lens on the microscope controls total magnification?

A

objective lens (bc ocular lens have a constant value, and objective lens vary, making it the controller of total magnification)

21
Q

What is the function of the objective lens?

Know where this is located

A

responsible for primary magnification of the image (depends on which objective lens you use)

22
Q

What is the function of the revolving nosepiece?

Know where this is located

A

used to change from one objective lens to the next objective

23
Q

Name the different types of objective lens, their magnification, and what knob you use when utilizing that specific lens.

A

Scanning OL: 4x - coarse knob and some fine focus knob

Low Power OL: 10x - fine focus knob

High Power OL: 40x - fine focus knob

Oil Immersion lens: 100x - fine focus knob

24
Q

What is the function of the ocular lens?

Know where this is located

A

always magnifies image to 10x; constant

25
Q

What is the formula for total magnification?

A

ocular lens magnification X objective lens magnification

26
Q

What is the total magnification using the high-power lens?

A

ocular lens = 10x
high power OL = 40x

40 x 10 = 400x

27
Q

What objective lens are you using if the total magnification is 100x?

A

total magniciation = 100x
ocular lens = 10x

100/10 = 10x = low power OL

28
Q

What is the function of the coarse adjuster/focus knob?

Know where this is located

A

moves stage up and down to achieve an initial/rough focus

(is the bigger, inner knob)

29
Q

What is the function of the fine adjuster/focus knob?

Know where this is located

A

allows only fine movement of stage to achieve a later, fine focus

(is the smaller, outer knob)

30
Q

Define field of vision

A

area you can see when looking through a microscope

31
Q

Describe the relationship between range of field (visual field) and magnification

Explain how magnification affects the visual field and observational size of specimen

A

inverse relationship

At 4x, the magnification is low, therefore the visual field is large. Specimen will appear “small”

Compared to 40x, the magnification is high, therefore the visual field is small. Specimen will appear “large”

32
Q

Define depth of field

A

distance between objective lens and specimen

33
Q

Describe the relationship between depth of field and magnification

Explain how magnification affects the depth of field

A

inverse relationship

At 40x, the magnification is high, therefore it will have a smaller depth of field.

Compared to 4x, the magnification is low, therefore it will have a high depth of field

34
Q

A specimen has several planes known as the…

A

planes of focus

35
Q

What plane is deal to focus on?

A

center plane

36
Q

Explain how the coarse focus knob and the fine focus knob help achieve an ideal plane of focus

A

coarse focus knob - gets you to any one of several planes of focus

fine focus knob - gets you to the center plane of focus = better resolution

37
Q

Describe blue light

A
  • short wavelength
  • penetrates deep into specimen
  • provides best resolution
38
Q

What light gives the best resolution?

A

blue light

39
Q

What does PH stand for regarding the field setting disk of a microscope setting?

A

phase contrast

BIO 220 - [Lab Quizzes] (4 decks)

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