Lab Quiz #1 - Microscope Quiz Flashcards
What is the definition of a compound microscope?
instrument consisted of at LEAST 2 lenses: ocular lens (eyepiece) and objective lens
(our microscopes also have a condenser lens)
What is the function of the compound microscope?
magnify the image; NOT the object
What is the definition of resolution?
ability of microscope to show fine structure details with clarity/sharpness
What is the function of the main/power switch?
Know where this is located
turns on light source
What is the function of the brightness adjustment knob?
Know where this is located
regulates amount of light to the microscope
(set it to comfortable eye setting)
What is the function of the light source/LED bulb
Know where this is located
provides white light
What parts of the microscope can you find at the base?
main/power switch j
brightness adjustment knob
(light source/LED bulb)
What is the substage assembly area?
area underneath the stage
What parts of the microscope can you find in the substage assembly area?
iris/aperture diaphragm with lever
condenser lens
What is the function of the iris/aperture diagram with lever
Know where this can be found
fine tunes the light
(slowly move lever to let light in)
What is the function of the condenser lens
Know where this can be found
prevents scattering of light as it travels from the bottom
seen through hole in stage and under specimen
Name all of the compound lenses in our compound microscope
Which lens does NOT contribute to magnification?
ocular lens
objective lens
condenser lens
Compare Bright Field Setting/Microscopy vs. Dark Field Microscopy: backgound; object color; light; specimens
BF setting/microscopy
- light background
- colored/dyed object
- light passes through specimen
- specimen is dead
DF setting/microscopy
- dark background
- object NOT colored; no dyes used
- light reflects off specimen = shiny appearance
- used for live specimens
Why is BF setting/microscopy used to observe dead specimens?
In BF setting/microscopy, we use it to observe colored/dyed specimens. dyes are toxins that will kill things, therefore, any specimen that is dyed has literally dieded 😂
What is the function of the stage?
Know where this is located
rest the slide/specimen on top of it
What is the function of the slide adjuster knob?
Know where this is located
moves slide back and forth and side to side to scan the slide starting at the lowest magnification (4x)
- part of stage -
What is the function of the scale of specimen position?
Know where this is located
are coordinates for positioning/pinpointing a landmark on the slide
- part of stage-
TRUE or FALSE: the scale of specimen position is used for measuring size of specimen
False, scale of specimen are coordinates for positioning/pinpointing a landmark on slide
TRUE or FALSE: Objective lenses have a constant magnification
False, objective lenses vary in magnification. Ocular lens have a constant magnification of 10x
Which lens on the microscope controls total magnification?
objective lens (bc ocular lens have a constant value, and objective lens vary, making it the controller of total magnification)
What is the function of the objective lens?
Know where this is located
responsible for primary magnification of the image (depends on which objective lens you use)
What is the function of the revolving nosepiece?
Know where this is located
used to change from one objective lens to the next objective
Name the different types of objective lens, their magnification, and what knob you use when utilizing that specific lens.
Scanning OL: 4x - coarse knob and some fine focus knob
Low Power OL: 10x - fine focus knob
High Power OL: 40x - fine focus knob
Oil Immersion lens: 100x - fine focus knob
What is the function of the ocular lens?
Know where this is located
always magnifies image to 10x; constant
