Lab Quiz 1 Flashcards
What are the required materials for lab?
Safety goggles with side shield, lab handout, close-toed-shoes, and internet device
When you enter and leave the room, what should you do?
Wipe table and wash hands
What do you need to wear for lab?
Safety goggles, close-toed shoes, legs covered
Where should you store food/drinks/backpacks?
At front of the classroom or in the cabinet
If an injury occurs, what should you do?
Notify instructor
If there is a spill or broken equipment, what should you do?
Notify instructor
Small chemical spill: what should you do?
napkins
Large chemical spill: what should you do?
Hazchem spill response kit, chemical shower
Emergency evacuation: what is the first thing you should do?
Secure station if time permits
Emergency evacuation: what are the steps?
Secure station if time permits, walk to designated exit and down stairwell, meet in lot 2. Gather at lot 2, then notify instructor of presence. Wait for signal for re-entry
What goes down the drain?
Nothing, except for water you wash your hands with
What goes in the trash?
Nothing, except for napkins you used to wash your hands with
Where do gloves go?
Burn box
What goes in the blue recycling bin?
Paper, aluminum foil, cardboard
What goes in the glass recycling bin?
Not broken glass
What goes into the glass recycling BUCKET?
Broken glass
What goes in the burn box?
Pipettes and gloves
What goes into the biohazardous waste bin?
bacterial cultures, used pipette tips with certain bacteria on them
Where do you dispose of liquid chemicals?
In the hood, into a waste jar
Blue part on the NFPA
Health Hazard:
4= can be lethal,
3= can cause serious injury,
2= can cause temporary incapacitation or residual injury,
1= can cause significant irritation
0=no hazard
Red part on the NFPA
Flammability Hazard:
4= will vaporize and burn at normal temperatures.
3= can be ignited at almost all ambient temperatures
2= must be heated or high ambient temperature to burn.
1= must be preheated before ignition can occur
0= will not burn
White part on the NFPA
Special hazard:
OX= oxidizing.
SA = simple asphyxiants
W crossed out = reacts violently or explosively with water
Yellow part on the NFPA
Instability hazard:
4= may explode at normal temperatures and pressures.
3= may explode at high temperature or shock
2 = violent chemical change at high temperatures or pressures
1= normally stable. high temperatures make unstable.
0= stable
base units of measurement
meter, liter, gram
When should you use a micropipette
when pipetting less than a mL
when should you use a p-1000?
when pipetting 100-1000 microliters
p-20
2 microliters to 20 microliters
how to set volume on a micropipette
gently pull up and turn black wheel, then push black wheel back into original position
before pipetting anything:
place a disposable plastic pipette tip
how to hold a micropipette
place tip onto micropipette by holding micropipette by handle and then putting the pipette tip-down, sliding it into the tip.
what does the plunger do?
fluid taken up into and expelled from the pipette tip by using the micropipette; push down on the button
plunger stops: how many
2 stops
how do you aspirate fluid into the pipette tip?
push the plunger button down to the first stop. continue to hold it as you place the pipette tip into a fluid you want. Only very end of pipette tip needs to be placed into fluid. Release button slowly to draw fluid into pipette. Keep pipette in the fluid until the plunger has completely regained original position. Draw tip out of the fluid into the microcentrifuge tube.
how do you expel fluid from the pipette tip?
place pipette into tube where you want to expel liquid, slowly push down on plunger from first stop to the second stop. Hold down button until pipette is no longer touching the fluid
what direction should you hold the pipette?
straight down
what speed should you let the plunger up?
slowly
relationship between transmittance and absorbance
absorbance = -log transmittance
when to use a P10
.5 microliters-10 microliter
where should you dispose of needles or razors?
Sharp disposal box (small and red), or orange and white burn-up bin
Where should you dispose of non-sharp biohazards?
Large, red bins with plastic biohazard bag inserts
