Lab Practical Exam 1 (all the quizzes) Flashcards
when using the 100x objective you should only use the ____ to make the image clearer.
A. coarse focus knob
B. condenser knob
C. fine focus knob
D. Iris diaphragm lever
fine focus knob
to increase resolution, we can add a drop of ____ to the slide.
A. 70% ethanol
B. immersion oil
C. mineral oil
D. water
immersion oil
what is NOT an example of a structural stain?
A. flagellar stain
B. capsule stain
C. endospore stain
D. simple stain
simple stain
gram staining works based on the difference of ___ between Gram negative and Gram positive cells
A. the DNA
B. the cell membrane
C. the cell wall
D. the capsule
the cell wall
which is the correct sequence of Gram staining procedure?
A. Crystal violet, iodine, decolorizer, safranin
B. iodine, crystal violet, decolorizer, safranin
C. crystal violet, safranin, decolorizer, iodine
D. safranin, iodine, decolorizer, crystal violet
crystal violet, iodine, decolorizer, safranin
what does “S” stand for in “SAM-(EMC)?”
Shape
what should NOT be done during a wet mount?
A. put a coverslip on slide
B. use 5 drops of water from source
C. draw organism
D. observe slide at 400x magnification
use 5 drops of water from source
a single cell or bacterium when duplicated enough can form a visible___.
A. streak
B. species
C. clump
D. colony
colony
using a wet mount with 100x total magnification, which group of organisms will we NOT be able to determine motility?
A. amoebozoa
B. protozoa
C. ciliophora
D. bacteria
bacteria
eukaryotes include all the following EXCEPT:
A. bacteria
B. amoebozoa
C. fungi
D. excavata
bacteria
where will you place your bacterial plates at the end of today’s class?
A. class bin for incubation at 37 degrees Celsius
B. biohazard bin
C. cardboard box for storage at 4 degrees Celsius
D. back on the bench for growth at room temperature
class bin for incubation at 37 degrees Celsius
all the following are true about prokaryotes EXCEPT:
A. have NO nuclear membrane
B. contain a true nucleus
C. are submicroscopic
D. lack sterols
contain a true nucleus
Titer = ?
N/DF*V
Which one is NOT an important factor in titer?
A. size of tube
B. number of colonies
C. volume
D. dilution factor
size of tube
in the titer equation, DF stands for…
the dilution factor
the liquid portion of the sample is called:
A. pellet
B. titer
C. dilution
D. supernatant
supernatant
in lab 2 of this exercise, you will count the number of colonies on your plates. which is the countable range of colonies you will be looking for?
30-300 CFU
how can we avoid a bacterial lawn when we perform a plate count?
A. perform serial dilution
B. sterilize the surface of an agar plate with 70% ethanol
C. incubate the plate at a low temperature
D. add herbicide to the agar side
perform serial dilution
MSA stands for:
Mannitol Salt Agar
___ might be added to selective media.
A. specific inhibitors
B. salt
C. antibiotics
D. dyes
E. all of above
all of above
the rich broth in the fluid thioglycollate is used for:
A. indicating oxygen
B. preventing contamination of the media
C. providing nutrients
D. minimizing oxygen diffusion rates
providing nutrients
while putting your needle in the Fluid Thioglycollate soft agar, you should:
keep needle straight while inserting it and removing it from the tube
gas production may be seen in which biochemical test?
A. MR-VP test
B. sugar fermentation tube
C. simmon’s citrate
D. MacConkey agar
sugar fermentation tube
in Bio221, what technique do you use to inoculate the simmon’s citrate test?
A. needle stab
B. spread plate
C. needle stabe streak
D. loop inoculation
needle stab streak
why is it important to use cultures that are 24-36 hours old for detecting catalase?
A. these cultures are metabolically inactive and easier to be tested
B. fresh cultures do not have catalase
C. these cultures are turbid enough, making the change of color easy to recognize
D. these cultures are metabolically active and more likely to produce catalase
these cultures are metabolically active and more likely to produce catalase
which of following enzymes can convert hydrogen peroxide into water and oxygen?
A. catalase
B. tryptophanase
C. deaminase
D. oxidase
catalase
the Phenylalanine Deaminase Test uses the indicator: ____, and a positive result turns ___.
ferric chloride; green
which of following descriptions is correct?
A. lipases are responsible for making lipids
B. amylases break down starch to sugars
C. helicases unravel the DNA helix
D. both amylases break down starch and helicases unravel the DNA helix
both amylases break down starch and helicases unravel the DNA helix
when you are finished with the swab at the end of Lab 1 for Enzyme Reactions, where will you place it?
biohazard bin
for lab 2 of Enzyme reactions, you should record all of the following EXCEPT:
A. organism name
B. number of colonies
C. growth
D. color
number of colonies
from what environmental source will we be extracting spores from?
A. hand swabs
B. water
C. soil
D. drainpipes
soil
what is NOT a stage of biofilm development?
A. active dilution
B. maturation
C. non-reversible attachment
D. reversible attachment
active dilution
active dispersal of the entrapped cells typically occurs when…
A. a disinfectant is used
B. the surrounding environment becomes more favorable
C. the cell membrane is destroyed
D. there is an increase in production of cell structures
the surrounding environment becomes more favorable
in the process of cytosine deamination, cytosine is converted to:
A. thymine
B. adenine
C. guanine
D. uracil
uracil
antiseptics are used on ____ while disinfectants are used on ___.
A. living surfaces, living surfaces
B. non-living surfaces, living surfaces
C. living surfaces, non-living surfaces
D. non-living surfaces, non-living surfaces
living surfaces, non-living surfaces
in the maturation stage of a biofilm, bacteria lose their….
A. cell wall
B. flagella
C. capsule
D. glycocalyx
flagella
