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BIOL 221 > Lab Practical Exam 1 (all the quizzes) > Flashcards

Lab Practical Exam 1 (all the quizzes) Flashcards

1
Q

when using the 100x objective you should only use the ____ to make the image clearer.
A. coarse focus knob
B. condenser knob
C. fine focus knob
D. Iris diaphragm lever

A

fine focus knob

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2
Q

to increase resolution, we can add a drop of ____ to the slide.
A. 70% ethanol
B. immersion oil
C. mineral oil
D. water

A

immersion oil

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3
Q

what is NOT an example of a structural stain?
A. flagellar stain
B. capsule stain
C. endospore stain
D. simple stain

A

simple stain

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4
Q

gram staining works based on the difference of ___ between Gram negative and Gram positive cells
A. the DNA
B. the cell membrane
C. the cell wall
D. the capsule

A

the cell wall

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5
Q

which is the correct sequence of Gram staining procedure?
A. Crystal violet, iodine, decolorizer, safranin
B. iodine, crystal violet, decolorizer, safranin
C. crystal violet, safranin, decolorizer, iodine
D. safranin, iodine, decolorizer, crystal violet

A

crystal violet, iodine, decolorizer, safranin

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6
Q

what does “S” stand for in “SAM-(EMC)?”

A

Shape

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7
Q

what should NOT be done during a wet mount?
A. put a coverslip on slide
B. use 5 drops of water from source
C. draw organism
D. observe slide at 400x magnification

A

use 5 drops of water from source

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8
Q

a single cell or bacterium when duplicated enough can form a visible___.
A. streak
B. species
C. clump
D. colony

A

colony

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9
Q

using a wet mount with 100x total magnification, which group of organisms will we NOT be able to determine motility?
A. amoebozoa
B. protozoa
C. ciliophora
D. bacteria

A

bacteria

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10
Q

eukaryotes include all the following EXCEPT:
A. bacteria
B. amoebozoa
C. fungi
D. excavata

A

bacteria

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11
Q

where will you place your bacterial plates at the end of today’s class?
A. class bin for incubation at 37 degrees Celsius
B. biohazard bin
C. cardboard box for storage at 4 degrees Celsius
D. back on the bench for growth at room temperature

A

class bin for incubation at 37 degrees Celsius

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12
Q

all the following are true about prokaryotes EXCEPT:
A. have NO nuclear membrane
B. contain a true nucleus
C. are submicroscopic
D. lack sterols

A

contain a true nucleus

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13
Q

Titer = ?

A

N/DF*V

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14
Q

Which one is NOT an important factor in titer?
A. size of tube
B. number of colonies
C. volume
D. dilution factor

A

size of tube

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15
Q

in the titer equation, DF stands for…

A

the dilution factor

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16
Q

the liquid portion of the sample is called:
A. pellet
B. titer
C. dilution
D. supernatant

A

supernatant

17
Q

in lab 2 of this exercise, you will count the number of colonies on your plates. which is the countable range of colonies you will be looking for?

A

30-300 CFU

18
Q

how can we avoid a bacterial lawn when we perform a plate count?
A. perform serial dilution
B. sterilize the surface of an agar plate with 70% ethanol
C. incubate the plate at a low temperature
D. add herbicide to the agar side

A

perform serial dilution

19
Q

MSA stands for:

A

Mannitol Salt Agar

20
Q

___ might be added to selective media.
A. specific inhibitors
B. salt
C. antibiotics
D. dyes
E. all of above

A

all of above

21
Q

the rich broth in the fluid thioglycollate is used for:
A. indicating oxygen
B. preventing contamination of the media
C. providing nutrients
D. minimizing oxygen diffusion rates

A

providing nutrients

22
Q

while putting your needle in the Fluid Thioglycollate soft agar, you should:

A

keep needle straight while inserting it and removing it from the tube

23
Q

gas production may be seen in which biochemical test?
A. MR-VP test
B. sugar fermentation tube
C. simmon’s citrate
D. MacConkey agar

A

sugar fermentation tube

24
Q

in Bio221, what technique do you use to inoculate the simmon’s citrate test?
A. needle stab
B. spread plate
C. needle stabe streak
D. loop inoculation

A

needle stab streak

25
Q

why is it important to use cultures that are 24-36 hours old for detecting catalase?
A. these cultures are metabolically inactive and easier to be tested
B. fresh cultures do not have catalase
C. these cultures are turbid enough, making the change of color easy to recognize
D. these cultures are metabolically active and more likely to produce catalase

A

these cultures are metabolically active and more likely to produce catalase

26
Q

which of following enzymes can convert hydrogen peroxide into water and oxygen?
A. catalase
B. tryptophanase
C. deaminase
D. oxidase

A

catalase

27
Q

the Phenylalanine Deaminase Test uses the indicator: ____, and a positive result turns ___.

A

ferric chloride; green

28
Q

which of following descriptions is correct?
A. lipases are responsible for making lipids
B. amylases break down starch to sugars
C. helicases unravel the DNA helix
D. both amylases break down starch and helicases unravel the DNA helix

A

both amylases break down starch and helicases unravel the DNA helix

29
Q

when you are finished with the swab at the end of Lab 1 for Enzyme Reactions, where will you place it?

A

biohazard bin

30
Q

for lab 2 of Enzyme reactions, you should record all of the following EXCEPT:
A. organism name
B. number of colonies
C. growth
D. color

A

number of colonies

31
Q

from what environmental source will we be extracting spores from?
A. hand swabs
B. water
C. soil
D. drainpipes

A

soil

32
Q

what is NOT a stage of biofilm development?
A. active dilution
B. maturation
C. non-reversible attachment
D. reversible attachment

A

active dilution

33
Q

active dispersal of the entrapped cells typically occurs when…
A. a disinfectant is used
B. the surrounding environment becomes more favorable
C. the cell membrane is destroyed
D. there is an increase in production of cell structures

A

the surrounding environment becomes more favorable

34
Q

in the process of cytosine deamination, cytosine is converted to:
A. thymine
B. adenine
C. guanine
D. uracil

A

uracil

35
Q

antiseptics are used on ____ while disinfectants are used on ___.
A. living surfaces, living surfaces
B. non-living surfaces, living surfaces
C. living surfaces, non-living surfaces
D. non-living surfaces, non-living surfaces

A

living surfaces, non-living surfaces

36
Q

in the maturation stage of a biofilm, bacteria lose their….
A. cell wall
B. flagella
C. capsule
D. glycocalyx

A

flagella

BIOL 221 (9 decks)

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