LAB PRACTICAL Flashcards
What are the reactants of photosynthesis?
6H2O + 6CO2
What are the products of photosynthesis?
C6H12O6 + 6O2 (byproduct)
What is the energy source for photosynthesis?
Sun light
Light is the result of a(n) ______ traveling in the pattern of a(n) _______.
Photons, wavelength
Where in the chloroplast does the light INDEPENDENT reaction occur?
Stroma
Where in the chloroplast does the light DEPENDENT reaction occur?
Thylakoids
What is the biological function of a stomata?
Allows for gas exchange
Why did chlorophyll A move the least amount along the chromatography paper?
Because it had a higher molecular weight
What is the function of the cuticle (waxy layer that surrounds leaf)?
Protects the leaf
PICTURE IDENTIFY THE PORTION OF THE MODEL WHERE MESOPHYLL CELL (PALISADE)
Mesophyll Cell — Palisade
What is the primary pigment used by photosynthesis?
Chlorophyll A
What is the ecological role of photosynthesis?
To convert light energy into chemical energy (food)
What is the purpose of phenol red pH indicator used to detect the presence of CO2 in water?
CO2 combined with water forms carbonic acid - phenol red turns yellow in an acidic solution and red in a basic solution - so when plants take up the CO2, the solution becomes more basic.
What is the net photosynthetic equation?
6 CO2 + 6 H2O —> C6H12O6 + 6 O2
What (supposedly) happened to CO2 levels in the containers full of germinated beans?
CO2 is a product that was released and is supposed to be reused in cellular respiration.
What molecule is the chief product of cellular respiration?
ATP
What is the cellular respiration equation?
C6H12O6 + 6O2 —> 6 H2O + 6 CO2 —- the breakdown of glucose to form ATP
Define Aerobic Respiration
the breakdown of glucose using O2 to form ATP by the 3 pathways of glycolysis, krebs cycle, and electron transport chain
What organic molecule is being split during the process of glycolysis?
Glucose
What element is known as the final hydrogen/electron acceptor?
O2
Where in the cell does glycolysis occur?
Cytoplasm
PICTURE************** Name the internal portion of the structure labled A.
Matrix
What occurs within the matrix of the mitochondria?
The Krebs Cycle
What is the name of the fold of the membrane within the mitochondria?
Cristae
What occurs along the cristae of the mitochondria?
Electron Transport Chain
PICTURE***************** What is the name of the entire structure?
Mitochondria
What are the substrates for cellular respiration?
C6H12O6 (glucose) + 6O2 (molecular oxygen)
What are the products of cellular respiration?
6H2O (water) + 6CO2 (carbon dioxide)
Where do the substrates for cellular respiration come from?
It can come from food which is made by autotrophs by taking in light energy (sun) then having photosynthesis occur which then produces glucose.
What molecule is being broken down to produce CO2 during fermentation?
Glucose
Where in the cell does glycolysis occur?
Cytoplasm
Where in the cell does the krebs cycle occur?
the matrix of the mitochondria
Where in the cell does the electron transport chain occur?
Along the cristae of the mitochondria
If you are in a sealed room, what would you think will happen with the O2 concentrations in the room over time?
The O2 will decrease because we (humans) breathe it in and use it for our bodies.
If you are in a sealed room, what would you think will happen with the CO2 concentrations in the room over time?
The CO2 will increase because it is what we release as a waste as humans by exhaling.
Cellular respiration is controlled by enzymes, so if you lowered the temperature, would you expect a different rate of production? Would the difference be a lower rate or increased rate?
Yes, lower rate.
Which of the five nucleotides is found only in DNA?
Thymine
Which of the five nucleotides is only found in RNA?
Uracil
How many nucleotides can be found with both RNA and DNA?
3 - adenine, cytosine, guanine
What type of bond forms between the 2 stands of DNA?
Hydrogen Bond
What is the term for a linear piece of DNA which contains multiple genes?
Chromosome (they’re linear in humans)
Genes hold information to build what other type of molecule?
Protein
A particular codon will code for what monomer?
nucleotide
What type of RNA contains the anti-codon?
tRNA
Which type of RNA is a structural component of a ribosome?
rRNA
For the non-sense strand: AGCTTA what is the the sense strand of DNA?
TCGAAT
For the non-sense strand: AGCTTA what is the mRNA molecule?
UCGAAU
For the non-sense strand: AGCTTA what is the tRNA molecule?
AGCUUA
Define chromosome.
structure that contains genes
Define gene.
a stretch of a chromosome which codes for a particular protein
Define genome.
the collection of all the genes found in all of the chromosomes for a particular organism
Transcription results in the formation of what molecule?
mRNA
Translation results in the formation of what molecule?
proteins
For the non-sense strand of DNA: ATGCTGGACCAG what is the sense strand?
TAC GAC CTG GTC
For the non-sense strand of DNA: ATGCTGGACCAG what is the mRNA molecule?
UAC GAC CUG GUC
For the non-sense strand of DNA: ATGCTGGACCAG what is the tRNA molecule?
AUG CUG GAC CAG
What is a codon?
3 nucleotides which encode one amino acid
Where would you expect to find the codons?
mRNA
What is an anti-codon?
the opposite code of that of the mRNA
Where does one find rRNA?
Ribosome
What was the purpose of salt to extract DNA from the strawberries?
to help the DNA appear and solidify
What was the purpose of dish soap to extract DNA from strawberries?
Pulls apart lipids and proteins from the cells so DNA can be released
Was there any protein found with the DNA after the DNA was extracted from the strawberries?
Proteins were found and indicated by a tan color
What is the term for the exchange of genetic material between 2 homologous chromosomes?
Crossing Over
What is being separated through the process of meiosis I?
Homologous Chromosomes
What is being separated through the process of meiosis II?
Sister Chromatids
What is the ploidy of the cells when meiosis is completed?
Haploid
What are the 3 main functions of mitosis (cell division)?
- Asexual Reproduction 2. Growth & Development 3. Repair & Cell Replacement
Which phase of mitosis is the process of separating duplicated chromosomes into 2 single chromosomes?
Anaphase
What is the name of the portion of a duplicated chromosome where the sister chromatids are attached to one another?
Centromere
Which phase of interphase results in the replication of DNA?
S Phase
Three ____ and one egg are produced during oogenesis.
Polar Bodies
PICTURE********* Identify the phase of mitosis in model.
PICTURE
PICTURE********* Identify the SPINDLE FIBERS on the model.
PICTURE
What is the main function of meiosis?
Create gametes
What happens in G1 Phase of interphase (3)?
- Cell Grows 2. Produces Proteins 3. Does normal cell metabolism
What happens in S Phase of interphase?
Cell replicates its DNA
What happens in the G2 phase of interphase?
Proteins for cell reproduction are produced.
At the end of meiosis, what do males produce?
4 Sperm Cells
At the end of meiosis, what do females produce?
1 Ovum, 3 polar bodies
What is the difference between diploid and haploid cells?
- Diploid - 2 sets of chromosomes 2. Haploid - 1 set of chromosomes
What is the objective during meiosis I?
to separate the homologous chromosomes
What is the objective during meiosis II?
to separate the sister chromatids
Define crossing over.
the exchange of DNA
Define homologous chromosomes
2 sister chromatids - chromosome pairs, each from one parent.
Three ____ and one egg are produced during oogenesis.
Polar Bodies
PICTURE********* Identify the phase of mitosis in model.
PICTURE
PICTURE********* Identify the SPINDLE FIBERS on the model.
PICTURE
What is the main function of meiosis?
Create gametes
What happens in G1 Phase of interphase (3)?
- Cell Grows 2. Produces Proteins 3. Does normal cell metabolism
What happens in S Phase of interphase?
Cell replicates its DNA
What happens in the G2 phase of interphase?
Proteins for cell reproduction are produced.
At the end of meiosis, what do males produce?
4 Sperm Cells
At the end of meiosis, what do females produce?
1 Ovum, 3 polar bodies
What is the difference between diploid and haploid cells?
- Diploid - 2 sets of chromosomes 2. Haploid - 1 set of chromosomes
What is the objective during meiosis I?
to separate the homologous chromosomes
What is the objective during meiosis II?
to separate the sister chromatids
Define crossing over.
the exchange of DNA
Define homologous chromosomes
2 sister chromatids - chromosome pairs, each from one parent.
A person with a diploid genotype AA Bb can produce gametes with what potential genotypes?
AB, Ab
If rolling your tongue consists of a dominant trait, what type of genotype do you have if you can’t roll your tongue?
Homozygous Recessive
The physical expression of one’s genes is known as their what?
Phenotype
Define genotype.
All of the actual alleles that makes up an organisms genome.
What is the basic unit of inheritance?
Gene
What type of allele is only expressed if there are 2 copies present?
Recessive
Who is the father of classical genetics?
Gregor Mendel
What is meant by the term homozygous dominant?
When an organism’s genotype has all the same alleles and the alleles are dominant, so they are expressed.
Can 2 heterozygous individuals produce offspring which show the recessive trait?
Yes
Draw a punnett square for Bb*Bb
B b B BB Bb b Bb bb
What is the genotypic ratio for the Punnet Square of Bb*Bb?
1:2:1
What is the phenotypic ratio for the Punnett square of Bb*Bb?
3:1
Why would a male have more problems with X-Linked recessive traits than a female?
Males only receive 1 set of Sex Linked genes because the Y chromosome only contains info. on being male so they are more likely to have sex linked recessive disorders
Define genotype
the actual alleles which make up an organisms genome
Define phenotype
physical expression of the organisms genetics
Define homozygous
a genotype where organisms have the same alleles for a particular gene
Define heterozygous
a genotype where organisms have different alleles
Define dominant alleles
alleles which express themselves if there is 1 or 2 copies of the allele
Define recessive alleles
alleles which only express if there are 2 copies of the recessive allele present
Define alleles
variations in genes
Define codominance
when 2 alleles express themselves
Define incomplete dominance
when an intermediate phenotype is the result of 2 different alleles
What is the multi-regional hypothesis?
various populations of Homo erectus around the world evolved into modern humans
What is the Out of Africa hypothesis?
a population of Homo erctus in Africa led to modern humans and eventually left Africa to the rest of the world
How does mutation affect the gene pool?
It allows for allele frequency to change
How does natural selection affect the gene pool?
individuals with the better phenotypes survive and reproduce and pass the alleles to their offspring, the frequency of these successful alleles will then increase in the gene pool
How does non-random mating affect the gene pool?
If there is a mating preference for a certain combination of alleles, the frequency of alleles in a population will change.
Define p^2
Frequency of the homozygous p genotype
Define 2pq
Frequency of the heterozygote genotype
Define q^2
Frequency of the homozygous q genotype
Define Biological Evolution
The change in allele frequencies over time
Define Genetic Equilibrium
When the 5 conditions of the Castle-Hardy-Weinber law are met: 1. No mutation 2. No gene flow 3. No new natural selection 4. Random mating 5. No genetic drift
What does the Castle-Hardy-Weignber Equation describe?
Demonstrates allele frequencies within a population
Name a trend in human evolution of the skull which is considered to be an advanced trait?
Larger brain - ancestors had smaller brains
Define p
frequency of the p allele in a population
Define q
Frequency of the q allele in the population
Is it easier for dominant or recessive alleles to be selected out of the population?
Dominant
What phase of mitosis is this?
Prophase
What phase of mitosis is this?
Metaphase
What phase of mitosis is this?
Anaphase
What phase of mitosis is this?
Telophase
What part of cell division is this?
Cytokinesis - 2 daughter cells