Lab practical 2

Question 1

Enterobacteriaceae Is a gram ______ rod.

Answer 1

• negative

Question 2

Macconkey only grows gram _____ rods

Answer 2

• negative

Question 3

Lactose Fermenters turn the colonies on Macconkey

Answer 3

• Pink

Question 4

Non-Lactose Fermenters have a ________ on macconkey agar

Answer 4

beige/white

Question 5

Heckteon agar grows what types of rods?

Answer 5

only grows gram negative rods

Question 6

Hecktoen is Used to differentiate species of ______________

Answer 6

Shigella vs. Salmonella

Question 7

Lactose Fermenters turn the colonies _______ on Hecktoen agar.

Answer 7

a bright orange color

Question 8

Non-Lactose Fermenter will keep the agar_________ on hecktoen agar

Answer 8

• green

Question 9

what is the TSI test used for?

Answer 9

used to differentiate enteric based on the ability to reduce sulfur and ferment carbohydrtates

Question 10

R/R =

Answer 10

no sugar fermentation

Question 11

R/Y (Red/Yellow) =

Answer 11

glucose is fermented but lactose and sucrose are not fermented

Question 12

Y/Y (Yellow/Yellow

Answer 12

Glucose ferments and sucrose and or lacctose also fermented

Question 13

R/Y With H2S (Red, black, yelow)

Answer 13

…

Question 14

SIM test stands for

Answer 14

Sulfide
Indole
Motility

Question 15

Positive sulfide test =

Answer 15

Black

Question 16

negative SUlfide test =

Answer 16

colorless

Question 17

indole positive result =

Answer 17

pink

Question 18

indole negative

Answer 18

colorless

Question 19

positive test for motility looks….

Answer 19

cloudy

Question 20

the negative test for motility =

Answer 20

line remains in the middle

Question 21

Urease (+)

Answer 21

neon pink

Question 22

Urease (-)

Answer 22

remains peachy color

Question 23

If the medium turns blue, the organism is _________________.

Answer 23

• citrate positive

Question 24

If there is no color change on the citrate agar, the organism is_________________.

Answer 24

• citrate negative

Question 25

Phenylalanine deaminase medium tests the ability of an organism…

Answer 25

to produce the enzyme deaminase.

Question 26

For the phenylalanine test, 10% ferric chloride is added to the media; if phenylpyruvic acid was produced, it will __________________.

Answer 26

react with the ferric chloride and turn dark green.

Question 27

If the medium remains ___________the organism is negative for phenylalanine deaminase production.

Answer 27

a straw color,

Question 28

what does a positive test look like for a positive MRVP test?

Answer 28

Red

Question 29

a dark copper color is reflective of a ________________ test for MRVP

Answer 29

negative

Question 30

Corynebacterium

Answer 30

Morphology: Gram (+) (PURPLE) Rods, Singly, in pairs, or characteristic palisade arrangement resembling rows of matchsticks.
(Review slides)

Question 31

Neisseria

Answer 31

Gram (-) (PINK) diplococci with adjacent sides flattened.

Review slides

Question 32

Clostridium

Answer 32

Gram (+) Rod (PURPLE)
Spore Forming – Straight/Slightly curved rods
(Review slides)

Question 33

Explain the the Kirby Bauer test…

Answer 33

Standardized test using a standard amount of organism, discs impregnated with define conc. of antibiotic, results are interpreted from tables where zone sizes are obtained from species with a known responsiveness for a specific antibiotic.