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Biology > Lab Practical 2 > Flashcards

Lab Practical 2 Flashcards

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1
Q

Net movement of substances from

A

High to low

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2
Q

Baggies with starch and iodine:
What is moved through the baggie?
What is the process?
How do you know the substance moved?

A
  • Iodine move through the baggies
  • the process is diffusion
  • blue black color indicates presence of both iodine and starch
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3
Q

Starch in beaker,iodine in baggie:
Which substance is in the beaker?
Which substance is in the baggie?

A
  • Both starch and iodine is in the beaker because of the blue color
  • iodine is in the baggie because of the brown color
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4
Q

Iodine in beaker, starch in baggie:
What substance is in the beaker?
What substance is in the baggie?

A
  • iodine is in the beaker because of the brown color
  • both is in the baggie because of the blue color
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5
Q

NaCl solution:
What are the two substances in the solution?
Which is the solute?
Which is the solvent?

A
  • Water and NaCl
  • NaCl (salt)
  • Water
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6
Q

Potatoes soaked in distilled water or in NaCl solution:
How does potato feel when soaked in distilled water?
Is distilled water hypotonic, isotonic, or hypertonic?
Potatoes lost or gain water?
Are potatoes turgid or plasmolyzed?

A
  • firm
  • hypotonic
  • gain
  • turgid
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7
Q

Potatoes soaked in distilled water or in NaCl solution:
How does potatoes feel in NaCl solution?
Is 10% NaCl hypotonic, isotonic, or hypertonic?
Potato cell gain or lose water?
Turgid or plasmolyze?

A
  • floppy
    -hypertonic
  • lose water
  • plasmolyze
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8
Q

Blood cells put in distilled water, 0.9% salt, or 10% salt water:
What solution is hypotonic to blood cells?
What solution is hypertonic to blood cells?

A
  • distilled water
  • 10% salt water
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9
Q

Blood cells put in distilled water, 0.9% salt, 10% salt water:
Blood cells gain or lose in hypotonic solution?
Cremated or hemolyzed?
Blood appears cloudy or clear?

A
  • gain
  • hemolyzed
  • clear
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10
Q

Blood cells put in distilled water, 0.9% salt, 10% salt water:
Blood cells gain or lose in hypertonic solution?
Crenated or hemolyzed?
Cloudy or clear?

A
  • lose
  • Crenated
  • Cloudy
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11
Q

Concentration of isotonic NaCl solution to blood cells?
Effect does isotonic solution have on cells?
What molecule moves in and out of the cell?
What process?

A
  • 0.9%
  • No effect
  • Water
  • Osmosis
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12
Q

Elodea leaf in distilled water and 10% salt water:
What solution is hypotonic?
What solution is hypertonic?

A
  • distilled water
    -10% salt water
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13
Q

Elodea leaf in distilled water and in 10% salt water:
Appearance of cell when lost water?
Hypotonic or hypertonic?
Water or 10% NaCl?
Turgid or plasmolyzed.

A
  • chloroplast clumps to the center
  • hypertonic
  • 10% NaCl
  • plasmolyzed
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14
Q

Elodea leaf in distilled water or salt water solution:
- Appearance when gained water?
- Hypertonic or hypotonic?
- Water or 10% NaCl?
- Turgid or Plasmolyzed?

A
  • Chloroplast on the edge of cell
  • Hypotonic
  • Water
  • Turgid
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15
Q
  • What molecule moved into or out of the elodea cells?
  • What is the process?
A
  • water
  • osmosis
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16
Q

Enzymes
2H2O2 —catalase—>2H2O +O2
- substrate?
- products?
- enzyme?

A
  • hydrogen peroxide
  • water + oxygen
  • catalase
17
Q

Enzymes:
- how do you know reaction had taken place?
- source of enzyme used?
- liquid product caused by reaction?
- gas product caused by reaction (in bubbles)?

A
  • bubble column
  • potato juice
  • water
  • oxygen
18
Q

Enzymes:
- what happened when an enzyme is denatured?
-temp that denatures most enzymes?
- does pH denature enzymes?

A
  • stops working
  • very hot temp
  • yes
19
Q

Factors affecting enzyme activity:
- hydrogen peroxide + catalase
- water + hydrogen peroxide
- catalase + sucrose

A
  • yes
  • no, no catalase
  • no, no substrate
20
Q

Factors affecting enzymes activity:
- catalase 0 °C + hydrogen peroxide
- catalase at room temp + hydrogen peroxide
- boiled catalase + hydrogen

A
  • yes
  • yes
  • no, denatured
21
Q

Factors affecting enzyme activity:
- catalase + hydrogen peroxide + water (pH 7)
- Catalase + hydrogen peroxide + HCl
- Catalase + hydrogen peroxide + NaOH

A
  • yes
  • no, denatured
  • no, denatured
22
Q

Factors affecting enzyme activity:
REMEMBER!!

A
  • The more enzyme means faster reaction
  • The more substrate means faster reactions to a certain point
23
Q

Experiment with liver and hydrogen peroxide:
- Does liver have catalase?
- how do you prove?
- Exergonic or endergonic reaction?
- how do you know?
- Is catalase reusable?
- how do you prove?

A
  • yes
  • by adding hydrogen peroxide
  • Exergonic
  • tube was warm
  • yes
  • by adding more hydrogen peroxide
24
Q

Photosynthesis:
- organelle involved in photosynthesis?
- general equation for photosynthesis?

A
  • chloroplast
  • H2O + CO2 - > glucose + O2
25
Q

Chromatography:
- purpose?
- solvent used?
- what are the 4 pigments?
- which pigment is most soluble?
- which pigment is least soluble?

A
  • separate pigments
  • chromatography solution
  • chlorophyll b, chlorophyll a, xanthophyll, beta-carotene
  • beta carotene
  • chlorophyll b
26
Q

Chromatography:
- how is rf calculated?
- which pigment has the lowest rf value?
- which pigment has the highest rf value?

A
  • distance of pigment / distance of solvent
  • chlorophyll b
  • beta carotene
27
Q

Fermentation:
- general equation for yeast fermentation?
- aerobically or anaerobically?
- what organism is fermenting?
- what gas is released during fermentation?

A
  • glucose —> ethanol + CO2
  • anaerobically
  • yeast
  • CO2
28
Q

Fermentation:
- Yeast + glucose
- Yeast + lactose
- Yeast + sucrose
- Yeast + water

A
  • yes
  • no, yeast is lactose intolerant
  • yes
  • no, no sugar
29
Q

Fermentation:
- what was the control?
- how to recognize which voltmeter fermented the best?
- what gas is in the bubble at the top of the fermenting test tubes.

A
  • water
  • largest air bubble
  • CO2
30
Q

Mitosis:
Remember!!

A

Interphase - chromatin
Prophase - sister chromatids
Metaphase - sister chromatids
Anaphase - daughter chromosomes
Telophase - daughter chromosomes

31
Q
  • Which are replicated chromosomes?
  • Which are non replicated chromosomes?
A
  • sister chromatids
  • daughter chromosomes

Biology (1 decks)

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