Lab Practical Flashcards
differential and selective medium with a neutral red dye (red/pink in acidic conditions, colorless in alkaline conditions) that inhibits growth of gram positives due to crystal violet dye and bile salts
MacConkey Agar
MacConkey Agar
differential and selective medium with a neutral red dye (red/pink in acidic conditions, colorless in alkaline conditions) that inhibits growth of gram positives due to crystal violet dye and bile salts
MSA
differential and selective medium with high concentration salt and a pH indicator, inhibits the growth of all species except members of the Gram positive Staphylococci genus because of the high salt concentration
differential and selective medium with high concentration salt and a pH indicator, inhibits the growth of all species except members of the Gram positive Staphylococci genus because of the high salt concentration
MSA
PEA
selective medium that allows growth of gram positives (particularly cocci) and inhibits the growth of gram negatives
selective medium that allows growth of gram positives (particularly cocci) and inhibits the growth of gram negatives
PEA
Voges-Proskauer Test
differential test that differentiates glucose metabolism patterns, specifically whether the bacteria can break down glucose to butanediol; positive results for this test are mutually exclusive of positive results for Methyl Red Test
differential test that differentiates glucose metabolism patterns, specifically whether the bacteria can break down glucose to butanediol; positive results for this test are mutually exclusive of positive results for Methyl Red Test
Voges-Proskauer Test
Methyl Red Test
differential test that determines whether a bacteria uses mixed acid fermentation pathway to ferment glucose, indicated by a color change from yellow to red
differential test that determines whether a bacteria uses mixed acid fermentation pathway to ferment glucose, indicated by a color change from yellow to red
Methyl Red Test
Indole Test
differential test that determines whether the bacteria has the enzyme tryptophanase to hydrolyze tryptophan
differential test that determines whether the bacteria has the enzyme tryptophanase to hydrolyze tryptophan
Indole Test
Citrate Test
test that determines whether the bacteria can use citrate as its sole carbon source
test that determines whether the bacteria can use citrate as its sole carbon source
Citrate Test
Blood Agar
this type of agar is a differential media that identifies the type of hemolysis an organism is capable of
this type of agar is a differential media that identifies the type of hemolysis an organism is capable of
Blood Agar
ability to partially degrade red blood cells is known as ____ and is indicated by a ___ discoloration underneath or around colonies on a blood agar plate
Alpha-hemolysis; green
ability to completely degrade red blood cells is known as ___ and is indicated by a ___ around colonies on a blood agar plate
Beta-hemolysis; clearing
inability to degrade red blood cells, indicated by unchanged blood agar plate
Gamma-hemolysis
Urease Test
a differential media with a pH indicator that tests whether the organism produces the enzyme which is used to hydrolyze urea into ammonia and carbon dioxide
a differential media with a pH indicator that tests whether the organism produces the enzyme which is used to hydrolyze urea into ammonia and carbon dioxide
Urease Test
Casease Test
a differential media that tests whether bacteria produce an enzyme that can hydrolyze casein into smaller molecules for use in an anabolic process
a differential media that tests whether bacteria produce an enzyme that can hydrolyze casein into smaller molecules for use in an anabolic process
Casease Test
TSI
a differential medium that tells whether an organism can ferment glucose and/or lactose or sucrose, whether the organism produces hydrogen sulfide and whether the fermentation produces gas
a differential medium that tells whether an organism can ferment glucose and/or lactose or sucrose, whether the organism produces hydrogen sulfide and whether the fermentation produces gas
TSI
the concentrations of sugars in the TSI are __ glucose, __ sucrose and ___ lactose
0.1%, 1%, 1%
Nitrate Reduction Test
differential media to determine whether the bacteria can reduce nitrate to nitrite (and in some cases, from nitrate to nitrite to nitrogen gas)
differential media to determine whether the bacteria can reduce nitrate to nitrite (and in some cases, from nitrate to nitrite to nitrogen gas)
Nitrate Reduction Test
Decarboxylase Test
a differential test that tests for dextrose fermentation and the presence of an enzyme that can remove carboxyl groups from specific amino acids, producing alkaline conditions and a color change from yellow to purple; requires mineral oil to prevent exposure to oxygen, promoting fermentation
a differential test that tests for dextrose fermentation and the presence of an enzyme that can remove carboxyl groups from specific amino acids, producing alkaline conditions and a color change from yellow to purple; requires mineral oil to prevent exposure to oxygen, promoting fermentation
Decarboxylase Test
DNase Test
a differential test for Staphylocci testing for deoxyribonuclease activity
a differential test for Staphylocci testing for deoxyribonuclease activity
DNase Test
Coagulase Test
a differential test for Staphylococci testing for whether the bacteria has the enzyme (name of the test) to make blood coagulate and wall off itself from the host’s protective mechanisms
a differential test for Staphylococci testing for whether the bacteria has the enzyme (name of the test) to make blood coagulate and wall off itself from the host’s protective mechanisms
Coagulase Test
Phenylalanine Deaminase Agar
differential test for the ability of Enterobacteriaceae species to convert phenylalanine into phenylpyruvic acid
differential test for the ability of Enterobacteriaceae species to convert phenylalanine into phenylpyruvic acid
Phenylalanine Deaminase Agar
Eosin-Methylene Blue Agar (EMB)
differential and selective test that allows growth of gram-negative bacteria but not gram-positive bacteria differentiates between colonies of lactose fermenting and non-lactose fermenting bacteria; positive result indicates lactose fermentation abilities
differential and selective test that allows growth of gram-negative bacteria but not gram-positive bacteria differentiates between colonies of lactose fermenting and non-lactose fermenting bacteria; positive result indicates lactose fermentation abilities
Eosin-Methylene Blue Agar (EMB)
Bile Esculin Test
differential test for whether the bacterial can hydrolyze esculin
differential test for whether the bacterial can hydrolyze esculin
Bile Esculin Test
Carbohydrate fermentation test
tests for ability to ferment a certain sugar and also gas production
tests for ability to ferment a certain sugar and also gas production
Carbohydrate fermentation test
