Lab Practical

Question 1

differential and selective medium with a neutral red dye (red/pink in acidic conditions, colorless in alkaline conditions) that inhibits growth of gram positives due to crystal violet dye and bile salts

Answer 1

MacConkey Agar

Question 2

MacConkey Agar

Answer 2

differential and selective medium with a neutral red dye (red/pink in acidic conditions, colorless in alkaline conditions) that inhibits growth of gram positives due to crystal violet dye and bile salts

Question 3

MSA

Answer 3

differential and selective medium with high concentration salt and a pH indicator, inhibits the growth of all species except members of the Gram positive Staphylococci genus because of the high salt concentration

Question 4

differential and selective medium with high concentration salt and a pH indicator, inhibits the growth of all species except members of the Gram positive Staphylococci genus because of the high salt concentration

Answer 4

MSA

Question 5

PEA

Answer 5

selective medium that allows growth of gram positives (particularly cocci) and inhibits the growth of gram negatives

Question 6

selective medium that allows growth of gram positives (particularly cocci) and inhibits the growth of gram negatives

Answer 6

PEA

Question 7

Voges-Proskauer Test

Answer 7

differential test that differentiates glucose metabolism patterns, specifically whether the bacteria can break down glucose to butanediol; positive results for this test are mutually exclusive of positive results for Methyl Red Test

Question 8

differential test that differentiates glucose metabolism patterns, specifically whether the bacteria can break down glucose to butanediol; positive results for this test are mutually exclusive of positive results for Methyl Red Test

Answer 8

Voges-Proskauer Test

Question 9

Methyl Red Test

Answer 9

differential test that determines whether a bacteria uses mixed acid fermentation pathway to ferment glucose, indicated by a color change from yellow to red

Question 10

differential test that determines whether a bacteria uses mixed acid fermentation pathway to ferment glucose, indicated by a color change from yellow to red

Answer 10

Methyl Red Test

Question 11

Indole Test

Answer 11

differential test that determines whether the bacteria has the enzyme tryptophanase to hydrolyze tryptophan

Question 12

differential test that determines whether the bacteria has the enzyme tryptophanase to hydrolyze tryptophan

Answer 12

Indole Test

Question 13

Citrate Test

Answer 13

test that determines whether the bacteria can use citrate as its sole carbon source

Question 14

test that determines whether the bacteria can use citrate as its sole carbon source

Answer 14

Citrate Test

Question 15

Blood Agar

Answer 15

this type of agar is a differential media that identifies the type of hemolysis an organism is capable of

Question 16

this type of agar is a differential media that identifies the type of hemolysis an organism is capable of

Answer 16

Blood Agar

Question 17

ability to partially degrade red blood cells is known as ____ and is indicated by a ___ discoloration underneath or around colonies on a blood agar plate

Answer 17

Alpha-hemolysis; green

Question 18

ability to completely degrade red blood cells is known as ___ and is indicated by a ___ around colonies on a blood agar plate

Answer 18

Beta-hemolysis; clearing

Question 19

inability to degrade red blood cells, indicated by unchanged blood agar plate

Answer 19

Gamma-hemolysis

Question 20

Urease Test

Answer 20

a differential media with a pH indicator that tests whether the organism produces the enzyme which is used to hydrolyze urea into ammonia and carbon dioxide

Question 21

a differential media with a pH indicator that tests whether the organism produces the enzyme which is used to hydrolyze urea into ammonia and carbon dioxide

Answer 21

Urease Test

Question 22

Casease Test

Answer 22

a differential media that tests whether bacteria produce an enzyme that can hydrolyze casein into smaller molecules for use in an anabolic process

Question 23

a differential media that tests whether bacteria produce an enzyme that can hydrolyze casein into smaller molecules for use in an anabolic process

Answer 23

Casease Test

Question 24

TSI

Answer 24

a differential medium that tells whether an organism can ferment glucose and/or lactose or sucrose, whether the organism produces hydrogen sulfide and whether the fermentation produces gas

Question 25

a differential medium that tells whether an organism can ferment glucose and/or lactose or sucrose, whether the organism produces hydrogen sulfide and whether the fermentation produces gas

Answer 25

TSI

Question 26

the concentrations of sugars in the TSI are __ glucose, __ sucrose and ___ lactose

Answer 26

0.1%, 1%, 1%

Question 27

Nitrate Reduction Test

Answer 27

differential media to determine whether the bacteria can reduce nitrate to nitrite (and in some cases, from nitrate to nitrite to nitrogen gas)

Question 28

differential media to determine whether the bacteria can reduce nitrate to nitrite (and in some cases, from nitrate to nitrite to nitrogen gas)

Answer 28

Nitrate Reduction Test

Question 29

Decarboxylase Test

Answer 29

a differential test that tests for dextrose fermentation and the presence of an enzyme that can remove carboxyl groups from specific amino acids, producing alkaline conditions and a color change from yellow to purple; requires mineral oil to prevent exposure to oxygen, promoting fermentation

Question 30

a differential test that tests for dextrose fermentation and the presence of an enzyme that can remove carboxyl groups from specific amino acids, producing alkaline conditions and a color change from yellow to purple; requires mineral oil to prevent exposure to oxygen, promoting fermentation

Answer 30

Decarboxylase Test

Question 31

DNase Test

Answer 31

a differential test for Staphylocci testing for deoxyribonuclease activity

Question 32

a differential test for Staphylocci testing for deoxyribonuclease activity

Answer 32

DNase Test

Question 33

Coagulase Test

Answer 33

a differential test for Staphylococci testing for whether the bacteria has the enzyme (name of the test) to make blood coagulate and wall off itself from the host’s protective mechanisms

Question 34

a differential test for Staphylococci testing for whether the bacteria has the enzyme (name of the test) to make blood coagulate and wall off itself from the host’s protective mechanisms

Answer 34

Coagulase Test

Question 35

Phenylalanine Deaminase Agar

Answer 35

differential test for the ability of Enterobacteriaceae species to convert phenylalanine into phenylpyruvic acid

Question 36

differential test for the ability of Enterobacteriaceae species to convert phenylalanine into phenylpyruvic acid

Answer 36

Phenylalanine Deaminase Agar

Question 37

Eosin-Methylene Blue Agar (EMB)

Answer 37

differential and selective test that allows growth of gram-negative bacteria but not gram-positive bacteria differentiates between colonies of lactose fermenting and non-lactose fermenting bacteria; positive result indicates lactose fermentation abilities

Question 38

differential and selective test that allows growth of gram-negative bacteria but not gram-positive bacteria differentiates between colonies of lactose fermenting and non-lactose fermenting bacteria; positive result indicates lactose fermentation abilities

Answer 38

Eosin-Methylene Blue Agar (EMB)

Question 39

Bile Esculin Test

Answer 39

differential test for whether the bacterial can hydrolyze esculin

Question 40

differential test for whether the bacterial can hydrolyze esculin

Answer 40

Bile Esculin Test

Question 41

Carbohydrate fermentation test

Answer 41

tests for ability to ferment a certain sugar and also gas production

Question 42

tests for ability to ferment a certain sugar and also gas production

Answer 42

Carbohydrate fermentation test