Lab Practical 1

Question 1

Culture

Answer 1

Living microbes

Question 2

Pure culture

Answer 2

Single species

Question 3

Aseptic transfer

Answer 3

Transfer of microbes from pure culture to sterile medium

Question 4

3 Media Forms

Answer 4

Broths
Agar slants
Plated media

Question 5

Broths

Answer 5

Used for growing fresh culture or large number of cells

Question 6

Agar slants

Answer 6

Used to grow stock cultures, can be refrigerated after incubation, maintain for several weeks

Question 7

Plated media

Answer 7

Used for obtaining isolation species

Question 8

What is instruments for transfer

Answer 8

Inoculating loops or needles

Question 9

Culture consisting two or more species

Answer 9

Mixed culture

Question 10

Commonly used to isolation technique

Answer 10

Streak plate

Question 11

Cells that have been sufficiently isolate?

Answer 11

Colony

Question 12

Plates that are used primarily for obtaining isolated/pure colonies

Answer 12

Agar plates

Question 13

To study morphological characteristics of the colonies

Answer 13

Colony Morphology

Question 14

5 Colonies shape

Answer 14

Circular
Rhizoid
Irregular
Filamentous
Spindle

Question 15

Colonies Margin

Answer 15

Smooth entire
Irregular/erose
Undulate
Lobate
Curled
Rhizoid
Filamentous

Question 16

5 Colonies elevations

Answer 16

Flat
Raised
Convex
Pulvinate
Umbinate

Question 17

Colonies Size

Question 18

Colonies size

Answer 18

Punctiform
Small
Moderate
Large

Question 19

Colonies texture

Answer 19

Smooth
Rough

Question 20

Colonies appearance

Answer 20

Glistening (Shinny)
Dull

Question 21

Colonies pigmentation

Answer 21

Non pigmented (ex: cream tan white)
Pigmented (ex: purple,red, yellow)

Question 22

Colonies Optical Property

Answer 22

Opaque
Translucent
Transparent

Question 23

Colonial Shape (Whole colony)

Answer 23

Round
Irregular
Filamentous
Rhizoid