Lab operations / safety Flashcards
Which of the following protective equipment must be worn (by law) when handling formaldehyde?
a. impervious apron
b. lab coat
c. scrub suit
d. white uniform
a. impervious apron
Which of the following may be disposed of in the sewer system according to EPA and CDC a. absolute alcohol
b. silver salts
c. pulverized tissue
d. blood
d. blood
How many mL of water should be added to 80mL of concentrated formaldehyde to obtain sol of 10% formalin?
10/100 = 80/x
x= 800 mL solvent
mL H2O? 800 - 80= 720mL water
The handling of biohazards is governed by the:
a. blood-borne pathogen standard
b. formaldehyde standard
c. laboratory standard
d. respiratory protection standard
a. blood-borne pathogen
HCl acid may be disposed of in the sink, provided it is:
a. diluted to 24%
b. less than 2N solution
c. neutralized to a pH btw 3 and 11
d. not a concentrated solution
c. neutralized to a pH btw 3 and 11
During microscopic review of H&E stained sections, it is noted that there is insufficient light on the slide. The best way to correct this is by:
a. changing to a higher objective
b. increasing the numerical aperture
c. lowering the substrate condenser
d. opening the iris diaphragm
d. opening the iris diaphragm
control the amount of light reaching the sp
Nontoxic, basic stain that are suspended in sterile water and injected into living animals are referred to as:
a. vital
b. in vitro
c. amphoteric
d. anionic
a. vital
Tissue can be fixed in the microwave oven because it causes:
a. rapid molecular movement
b. reaction between protein molecules
c. ionizing radiating
d. rupture of hydrogen bonds
a. rapid molecular movement
Before adjusting the pH of the Warthin-Starry staining solutions, the pH meter should be calibrated with a buffer solution of pH:
a. 4
b. 6
c. 7
d. 10
a. 4 pH
Because Warthin-Starry staining solutions (spirochetes) contain acidified water at pH 4
A gradual increase in staining time, with worsening overstaining, is noted when using a microwave oven equipped with a temperature probe. The first step in correcting this problem would be to:
a. purchase a new temperature probe
b. have the microwave oven repair
c. clean the temperature probe
d. reposition the stainig container
c. clean the temperature probe
note: its probably not reaching the req temp right therefore more staining time was added resulting in overstaining
When fractional distillation solvent recovery sys are used, xylene:
a. can be recycled only a limited number of times
b. will be higher purity recycled than when original
c. can not be completely separated from alcohol
d. that is recovered should not be used for clearing
b. will be higher purity recycled than when origina
When a solution containing HCl acid is to be discarded in the sink, it should be:
between pH3 and pH11
The Microscopic review of slides stained for the presence of microorganisms require the used of immersion oil. The purpose of the oil is to:
a. enhance the color contrast
b. decrease refractive index
c. improve resolution
d. require less light intensity
c. improve resolution
Chronic repetitive trauma
Tingling or numbness in the hands, an aching neck, shoulder and wrist pain
To meet inspection criteria, the Lab is expected to prepare procedure manuals to conform tao the guidelines published by the:
a. CDC
b. CAP
c. JCAHO
d. NCCLS
d. NCCLS
National Committee for Clinical Laboratory Standards
The function of the condenser on a light microscope is to:
a. concentrate light on the tissue specimen
b. magnify and resolve the image
c. regulate the intensity of the light illuminating the specimen
d. furnish strongly divergent ligth to the specimen
a. concentrate light on the tissue specimen
The possibility of a repetitive motion injury (RMI) may be reduced by:
a. using a rocking motion on the flywheel when cutting paraffin sections
b. tightly gripping the forceps when embedding and coversliping
c. removing the lift each cassette with the left thumb
d. utilizing an automated coversliping system
d. utilizing an automated coversliping system
When an inorganic substance is dissolved in water, the usual result is:
a. the Tyndall effect
b. colloidal despersion
c. ionization
d. electrolysis
c. ionization
100 mL of a 1% sol of Luxol fast blue is needed. he old, stock dye had a concentration of 80% and the new preparation has a concentration of 84%. To achieve the same consistency in staining as with the old dye how many grams of the new dye are required?
Gravimetric factor=
old / new
80/84= .952
1%/100= x/ 100 ml x= 1
amount needed g= .952 x 1
= .952 g of new dye
A visible image is achieved in the TEM microscopy when:
a. electrons passing through the specimen collide with a fluorescent-coated viewing screen
b. an electron–sensitive photographic emulsion is applied to the viewing screen
c. ultraviolet light is introduced to the viewing screen
d. electrons passing though the object split and emit light in the vososble light range
a. electrons passing through the specimen collide with a fluorescent-coated viewing screen
A change in the pH of a sol from 7 to 6 indicates that the solution now contains:
a. double the # of hydronium ions as before
b. 5 times the # of hydronium ions as before
c. 10 times the # of hydroniums ions as before
d. double the # of hydroxyl ions as before
c. 10 times the # of hydroniums ions as before
NOTE
Ph # difference by 10.
Acid= more hydronium
Basic= more hydroxyl
The # of hrs used to calculate the annual salary of a full time employee is:
2080 hrs
CAP requieres that 90% of routine sp requiring frozen sections have a turn-around time of:
(from arrival to gross are to notification of dx)
20 min
Main regulatory body that defines anatomic pathology laboratorie’s accrediting regulations
CAP= college of american pathologist
an organization that accredits and certifies healthcare organizations that is recognized world wide
JCAHO= joint commission on accreditation of healthcare organizations
regulates and establishes sets of quality standards used by laboratories
CLIA= Clinical laboratory improvements amendements
an organization for pathologist and laboratory professionals that also certifies these individuals through the boad of certification BOC
ASCP
OSHA
The Occupational Safety and Health Administration
(under dep. labor)
-air contaminant standards formaldehyde standards -laboratory standad (exposuse to hazardous chemicals) -hazard communication standard (right to know) -bloodborne pathogens standard -repiratory standard -persoal protective equipment standard -hazardous waste operations
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DOT
Department Of Transportation
shipping hazardous materials
CAP
certifies pathologists and organizes laboratory inspections (lab safety)
CLIA
Clinical Laboratory Improvement Act/ ammendment
- stats who can perform specific jobs within a lab and how much education it required
EPA
Environmental Protection Agency
- laws for disposal of chemicals down the drain
NRC
Nuclear regulatory Commission
- guidelines to prevent exposure of workers to radiation
