Lab Investigation of Cardiac Disease Flashcards
What are the roles of biochemical tests in clinical medicine?
- Screening (subclinical conditions)
can assess if at risk of heart disease using cholesterol
(HDL,LDL) - Diagnosis (normal vs abnormal values)
- Monitoring (course of disease)
- Clinical management (treatment/ response)
- Prognosis (Risk stratification)
Describe the analytical characteristics of ideal markers
- Measurable by cost-effective method
- Simple to perform
- Rapid turnaround time - quick results
- Sufficient precision & accuracy
What are the clinical characteristics of ideal markers
- Early detection of disease
- Sensitivity vs specificity
- Validated decision limits
- Selection of therapy
- Risk stratification
- Prognostic value
- Ability to improve patient outcome
What is cardiovascular disease?
Umbrella term for a number of linked pathologies including:
- Coronary heart disease (CHD)
- Cerebrovascular disease
- Peripheral arterial disease
- Rheumatic + congenital heart diseases
- Venous thromboembolism
- Lymphatic disease
What is the clinical significance of Coronary heart disease?
CHD causes the most deaths in men and women from CVD especially through:
- Myocardial Infarction
- Heart Failure
How does an MI originate?
MI starts off as a coronary vessel disorder where atherosclerosis and plaque has developed
Long process over decades
Describe the process of developing myocardial infarction
- Initial lesions (endothelial cell dysfunction)
- Fatty streaks from cholesterol and fat build up which
calcifies - Extensive lipid accumulation and deposition
- Fibroatheroma - lipid core capped and fibrotic
- Fibroatheroma breaks through endothelium to become
a complicated lesion into vessel lumen
→ initiates thrombosis and coagulation = occlusion of vessel
Describe the epidemiology of MI occurrence depending on age
<20 y ~ 20% already have significant coronary atherosclerosis and lesions
20-29 y ~ 50%
30-39 y it’s up to 65%
What is atherosclerosis?
A complex inflammatory disease where plaque builds up inside your arteries
Outline the initiation of atherosclerosis
- Endothelial activation
- LDL penetrates endothelium
- LDL retained in intima, where it undergoes oxidative modification
- Proinflammatory lipids released from LDL
- Stimulates endothelial cells to express adhesion molecules
- Circulating monocytes adhere to endothelial cells expressing adhesion molecules e.g. VCAM-1
In atherosclerosis, What is the purpose of monocytes sticking to endothelial cells expressing adhesion molecules?
This enables them to migrate into intima due to increased permeability between endothelial cells (dysfunction)
What happens to monocytes once in the intima?
Monocytes differentiate into macrophages once migrated to the intima
Signalling molecules upregulate receptors on macrophage surfaces causing higher LDL uptake
Outline how calcification occurs in the intima
Foam cells accumulate from macrophages and are calcified
Proinflammatory response within vasculature from macrophages release cytokines, interleukins and interferons to exacerbate the response (T cell influx as well)`
How does coronary thrombosis occur?
Occlusion in a vessel due to deposition of cholesterol leads to atheroma formation that initiates thrombosis
What are the consequences of coronary thrombosis?
Ischemia → necrosis → myocardial infarction
Why does ischaemia occur in the heart?
Significant ischaemia occurs due to lack of oxygen to a part of the heart (necrosis)
How does ischaemia and necrosis lead to a myocardial infarction?
Insufficient fibrinolysis enables a myocardial infarction to ensue due to lack of perfusion
(necrosis of myocardial tissue)
How can atherosclerosis lead to Myocardial infarction?
Plaque rupture can lead to myocardial infarction
Describe the process of chest pain to heart attack
Angina → acute coronary syndromes → heart attack
What causes angina?
Angina: atherosclerosis present but sufficient flow to compensate
Chest pain but no cardiomyocyte necrosis
Why is it important to distinguish between types of IHD?
It’s important to define the type of Ischemic Heart Disease for:
Stable angina vs. acute myocardial infarction
- Treatment
- Prognosis
- Management
Outline the various causes of chest pain
- Broken rib
- Collapsed lung
- Nerve infection (shingles)
- “Pulled” muscle
- Infection
- Heartburn (hernia)
- Blood clot in the lungs (PE)
- Pericarditis
- Angina
- Myocardial infarction