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MEDL 200 > LAB GLASSWARE > Flashcards

LAB GLASSWARE Flashcards

1
Q

Tolerance and accuracy

A

Limits of error; nearness to the true value

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2
Q

Precision and reproducibility

A

Ability to repeatedly produce the same value

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3
Q

Capacity in terms of glassware

A

Volume the glassware will hold or deliver

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4
Q

Temperature in regards to glassware

A

Temp the glassware was calibrated and temp to be used at

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5
Q

What’s the average temperature for glassware to be calibrated at?

A

20c

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6
Q

Liquid ____ when warmed.

A

Expands

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7
Q

Liquid ____ when cooled.

A

“Contracts”

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8
Q

TC?

A

To contain

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9
Q

What does TO CONTAIN mean?

A

Holding a specific volume

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10
Q

TD?

A

To deliver

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11
Q

What does TO DELIVER mean?

A

Holding a bit more than what is stated; this is to deliver the stated amount

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12
Q

What is prepared in TC glassware?

A

Solutions

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13
Q

What are some examples of TC glassware?

A

Volumetric flasks

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14
Q

What glasswares are TD?

A

Pipettes and some graduated cylinders

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15
Q

Where should the meniscus be?

A

The lowest part of the meniscus should be ON the calibration mark.

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16
Q

What are volumetric flasks primarily used for?

A

Preparation of accurate solutions — reagents and controls

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17
Q

What are cylinders used for?

A

Prepare solutions that require less accuracy — ex. Cleaning solutions

18
Q

Are cylinders TC or TD?

A

Both

19
Q

What are examples of glassware that aren’t calibrated TC or TD—graduation marks are approximations?

A

Beakers, Erlenmeyer flasks, test tubes, and reagent bottles

20
Q

Volumetric pipettes are ____ (TC/TD).

A

TD

21
Q

Which is the most accurate pipette?

A

Volumetric

22
Q

What are some examples of graduated/measuring pipettes?

A

Mohr and serological

23
Q

What’s the difference between a Mohr and serological pipette?

A

Mohr pipettes aren’t calibrated at the tip while serological pipettes are.
Serological pipettes aren’t wiped with a tissue!

Mohr - you discard the liquid at the tip
Serological - you blow out the last drop

24
Q

What’s the ranking of pipettes in terms of accuracy?

A

Volumetric
Mohr
Serological
Pasteur

25
Q

What are serological pipettes used for?

A

Serial solutions; measuring thick liquids; procedures where accuracy doesn’t matter

26
Q

What are semiautomatic pipettes used for?

A

The measuring of small volumes (1uL - 1000uL).

27
Q

What are good techniques for using semiautomatic pipettes?

A
  • pipette held vertically
  • smooth depression and release of plunger
  • depress plunger before inserting into sample
  • when transferring to a receiving tube, don’t release plunger till you remove from receiving tube
28
Q

What are examples of semiautomatic pipettes?

A

MLA pipettes, and Eppensorf/Hamilton pipettes

29
Q

What’s the difference between a MLA pipette and an Eppendorf/Hamilton pipette?

A

The eppendorf/Hamilton pipette’s plunger has two stops

30
Q

How do you proper usage of the two stops on an eppendorf/Hamilton pipette?

A
  • first stop for picking up sample

- depress plunger to first stop when transferring to receiving tube, wait one second then depress to second stop

31
Q

How do you maintain a MLA pipette?

A
  • Clean and lubricate the piston every 3 months.
  • apply thin coat of silicone to piston.
  • replace seal if pipette doesn’t pick up or discharge samples
  • clear nozzle with compressed air jet or fine wire if clogged
  • calibrate
32
Q

How often should calibration and quality control checks be done on eppendorf/Hamilton pipettes?

A

Every 6 months or after maintenance

33
Q

What are some examples given for specialty pipettes?

A

Repeater, multi-tip, and digital

34
Q

What are repeater pipettes?

A

Pipettes that draw up a large volume, and dispenses some into multiple tubes

35
Q

What are multi-tip pipettes?

A

Pipettes that have many tips attached to one pipette to deliver the same volume into multiple sample cups at once

36
Q

What are digital pipettes?

A

Pipettes that have digital volume displays

37
Q

How do you check if glassware is physically clean?

A

Water will uniformly wet the surface of the glass

38
Q

How do you check if glassware is chemically clean?

A

By measuring the pH of water added to the glassware

Or by adding dilute indicator to the surface of the glass

39
Q

How many rinses with tap water do you need to do to manually clean glassware?

A

7-10

40
Q

How many rinses do you need to do with distilled water when manually cleaning glassware?

A

3

41
Q

How many cycles does the laboratory glassware washers have?

A

6

42
Q

What are the six cycles the laboratory washer goes through?

A
  1. Pre-rinse
  2. Detergent wash

3, 4, 5. First, second, and third rinses
6. Distilled or deionized water rinse

MEDL 200 (4 decks)

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