Lab Final

Question 1

Which type of fungi has lots of macroconidia?

Answer 1

Microsporum

Question 2

Which type of fungi has lots of microconidia?

Answer 2

Trichophyton

Question 3

When do you use TSA agar?

Answer 3

General purpose

Question 4

When do you use blood agar?

Answer 4

Fastidious organisms (need enriched media)

Question 5

What diagnostic characteristic does blood agar let us utilize?

Answer 5

Hemolysis

Question 6

When type of gram bacteria would you use MacConkey agar for?

Answer 6

G –

Question 7

What diagnostic characteristic does MacConkey agar let us utilize?

Answer 7

Lactose fermentation

Question 8

What aspects of MacConkey agar inhibit G+ bacteria?

Answer 8

Bile Salts, Crystal Violet

Question 9

What aspect of MacConkey agar stain the lactose?

Answer 9

neutral red dye

Question 10

Non lactose fermenters that grow on MacConkey utilize what nutrient?

Answer 10

Peptone

Question 11

Lac- bacteria that grow on MacConkey?

Answer 11

Salmonella, Proteus, Pseudomonas, Shigella

Question 12

Lac+ bacteria that grow on MacConkey? (just the three specifically addressed in lab)

Answer 12

E. coli, Enterobacter, Klebsiella

Question 13

“Primary stain” of Gram Stain process?

Answer 13

Crystal Violet

Question 14

“Mordant stain” of Gram Stain profess?

Answer 14

iodine

Question 15

Decolorizer for gram staining?

Answer 15

Alcohol or acetone

Question 16

“Counterstain” for gram staining?

Answer 16

Safranin/Fuschin

Question 17

What does crystal violet stain for gram staining?

Answer 17

BOTH G- and G+

Question 18

What does Safranin/Fuschin stain for gram staining?

Answer 18

G- ONLY (stains pink)

Question 19

What is catalase used for?

Answer 19

Differentiate Staph from Strept

Question 20

Is Staph or Strept Cat+?

Answer 20

Staph

Question 21

What does oxidase test test for?

Answer 21

Cytochrome C Oxidase

Question 22

What does catalase test for?

Answer 22

conversion of H202 into H20 and 02

Question 23

Is pseudomonas or strept Ox+?

Answer 23

Pseudomonas

Question 24

What agent is added for the motility test?

Answer 24

Tetrozolium salts

Question 25

What are tetrazolium salts converted to by motile bacteria?

Answer 25

Formazan (red color)

Question 26

What is the indicator for Urease test?

Answer 26

phenol red

Question 27

What color is negative/positive for the urease test?

Answer 27

Yellow- NegativeRed- Positive

Question 28

What antimicrobial susceptibility tests allow us to get an MIC?

Answer 28

E-test and Broth Microdilution

Question 29

Disk diffusion antimicrobial susceptibility test?

Answer 29

Kirby-Bauer

Question 30

Micro-ID test is used to differentiate which group of bacteria?

Answer 30

Enterobacteriaceae

Question 31

What McFarland standard is used for antimicrobial susceptibility?

Answer 31

0.5

Question 32

What is the colony forming units/mL for the 0.5 McFarland?

Answer 32

1.5x10^8

Question 33

Which antimicrobial susceptibility test utilizes Mueller-Hinton?

Answer 33

Broth Microdilution

Question 34

Competitive ELISA tests for what?

Answer 34

Antigen

Question 35

Indirect ELISA tests for what?

Answer 35

Antibody

Question 36

Sandwich ELISA tests for what?

Answer 36

Antigen

Question 37

Which IFA test is brighter?

Answer 37

Indirect