Lab Exam (From review sheet) Flashcards

1
Q

What is a hematology assay used for?

A

Assays are used to diagnose blood disorders such as anemias and other general diseases

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2
Q

CDCs: when and why

A

CBCs are performed when a patient feels symptoms such as weakness, fatigue, fever, bleeding, etc. The purpose is to see what specifically is wrong with the patient

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3
Q

CBC: components

A

a. RBC
b. WBC
c. Platelet
d. MCV
e. MCH
f. MCHC
g. PTT
h. aPTT

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4
Q

Reticulocyte units

A

Normally in %, but also in cells per liter for ARC

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5
Q

Sedimentation rate: what does it measure

A

Used to see if any disease affects the plasma proteins

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6
Q

Sedimentation rate: units

A

Millimeters

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7
Q

Sedimentation rate: factors that affect it

A

gender, habits like diet and drug use

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8
Q

Sedimentation rate: Prefered method

A

Westergren method

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9
Q

Sedimentation rate: Men Value

A

Male: 0-15mm

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10
Q

RBC Indices: MCV range

A

MCV: 80 to 100 femtoliter

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11
Q

Most important concern when performing a phlebotomy?

A

The main concern when taking a person’s blood sample is performing diplomacy and ethical behavior

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12
Q

Where do you place tourniquet

A

The tourniquet should be placed below IV site on the bicep and should be on for no longer than a minute

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13
Q

The order of the draw

A

Coagulation test (Light Blue top), Chemistry test (Red top), CBC test (Lavender top)

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14
Q

Tube types: what they’re for

A

Blue top: Coagulation
Red Top: Chemistry
Lavender: CBC

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15
Q

Tube types: what they’re for

A

Blue top: Coagulation
Red Top: Chemistry
Lavender: Hematology
Yellow: Microbiology

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16
Q

Common veins for phlebotomy

A

Median cubital vein

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17
Q

Where to draw/not draw blood sample

A

Draw blood on opposite arm above the IV site, don’t puncture at IV site

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18
Q

When to remove tourniquet

A

Remove the tourniquet one last tube starts filling

19
Q

How to handle needle

A

Secure with 3 fingers angled at 15 degree angle entering 3/4s of the needle in the skin

20
Q

Criteria for rejection of blood sample

A

a. Incorrect specimen collection container.
b. insufficient specimen quantity.
c. Transported incorrectly.
d. Inadequate fixative.
e. specimen too large for container.
f. Incorrect media.
g. Specimen stability compromised

21
Q

Hematocrit

A

volume occupied by RBCs in a given in a volume of blood, expressed in percentage of the whole blood sample

22
Q

Hemoglobin

A

the component of the RBC that transports O2 from lungs to tissues and CO2 from tissues to lungs

23
Q

Hematocrit calculation

A

RBC * MVC / 10 = HCT

24
Q

hematocrit value: infant

A

Newborn: 53-65 %

25
Q

hematocrit value: Child

A

Infant: 30-43 %

26
Q

hematocrit value: Women

A

37-47%

27
Q

hematocrit value: Men

A

42-52%

28
Q

What is the rule of 3?

A

Hematocrit = 3*hemoglobin
Hemoglobin= 3 * RBC

29
Q

Hemoglobin types

A

Oxyhemoglobin: with O2
Carbaminohemoglobin: with CO2
Methemoglobin: with ferric Iron
Carboxyhemoglobin: with CO
Sulfhemoglobin: with S

30
Q

Which types of hemoglobin doesn’t carry O2

A

Methemoglobin: with ferric Iron
Carboxyhemoglobin: with CO
Sulfhemoglobin: with S

31
Q

Hemoglobin affinity

A

250x stronger for carbon monoxide than O2

32
Q

Hemoglobin value: infant

A

13.5-19.5

33
Q

Hemoglobin value: Child

A

11.5-15.5

34
Q

Hemoglobin value: Women

A

12-15

35
Q

Hemoglobin value: Men

A

13.5-18

36
Q

MCV calculation

A

MCV: HCT/RBC *10

37
Q

MCH calulation

A

MCH: Hgb/RBC * 10

38
Q

MCHC calculation

A

MCHC: Hgb/HCT *100

39
Q

What does stain for Reticulocytes

A

Stains the RNA and ribosomes that are still present in reticulocytes

40
Q

Reticulocyte calculation

A

of reticulocytes counted/1000*100

41
Q

Sedimentation rate: Women Value

A

Female: 0-24mm

42
Q

Sedimentation rate: Child Value

A

Child: 0-24mm

43
Q

RBC Indices: MCH range

A

MCH: 27 to 31 picograms/cell

44
Q

RBC Indices: MCHC range

A

MCHC: 32 to 36 grams/deciliter