Lab Exam Flashcards (Monday)

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1
Q

You will NOT be able to participate in lab unless you have?

A
  1. Proper Face Covering
  2. Closed-Toed Shoes
  3. Long Pants
  4. Lab Coat
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2
Q

Safety goggles are required when working with hazardous materials. (True or False?)

A

True

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3
Q

Never try to pick up broken pieces with your hands. (True or False?)

A

True

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4
Q

Report ALL spills or other incidents immediately to your lab instructor. (True or False?)

A

True

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5
Q

Discard clean broken glass into the Biohazard Bin. (True or False?)

A

False– (Discard clean broken glass into the “Broken Glass” box ONLY!!)

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6
Q

Return all equipment to their designated locations and clean your work area. (True or False?)

A

True

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7
Q

Dry biohazardous waste should be disposed in?

A

A red biohazard bag, which is placed in a bin or container with biohazard labels on it.

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8
Q

Sharps should be disposed in a biohazard bin. (True or False?)

A

False– (Sharps should be disposed in a sharps container.)

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9
Q

Determine each object and match them to their appropriate locations.

  • Contaminated cultures
  • Petri dishes
  • Culture flasks
  • Gloves
  • Paper Towels
  • Contaminated hypodermic needles
  • Clean broken glassware (beakers, flasks)
  • Contaminated glass waste
  • Plastic pipette tips
  • Contaminated blades
  • Contaminated microscope slides
  • Contaminated scalpels
  • Any contaminated material which can puncture or penetrate the skin or a red bag
  • Solid materials that have been contaminated with animal blood
  • Kim wipes
  • Bench papers contaminated with biohazardous materials
  • Waste from infectious agents, such as bacteria, viruses or live or attenuated vaccine
  • Waste contaminated with excretion or secretion from infectious humans or animals
A
  1. Biohazard Bin
  2. Biohazard Bin
  3. Biohazard Bin
  4. Biohazard Bin
  5. Biohazard Bin
  6. Sharps Container
  7. Broken Glass Container
  8. Sharps Container
  9. Biohazard Bin
  10. Sharps Container
  11. Sharps Container
  12. Sharps Container
  13. Sharps Container
  14. Biohazard Bin
  15. Biohazard Bin
  16. Biohazard Bin
  17. Biohazard Bin
  18. Biohazard Bin
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10
Q

ALL pathological waste should be disposed of in a Pathological Waste Container. What are some examples of pathological waste?

A

Some examples include:
- Organs, tissues, body parts, and fluids, which have been removed by trauma, surgery, other medical procedures.
- Human or animal tissues injected with a human pathogen or are potentially infectious.

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11
Q

Where is liquid waste disposed of?

A

Through the conventional sanitary sewage system if the materials are deactivated with 10% bleach solution and copious amounts of water.
Examples:
- Human or animal blood
- Body fluid or semi-liquid materials

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12
Q

You prepared a Gram-stained slide of bacteria during your lab. How would you dispose of it at
the end of the lab?

A

Sharps Container

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13
Q

Your lab-mate accidentally breaks a beaker you used for boiling water in one of your experiments. Where would you CAREFULLY throw away the broken glass?

A

Broken Glass Container

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14
Q

What are five PPE requirements that must be worn during lab?

A
  1. Lab Coat
  2. Close-toed shoes
  3. Long pants
  4. Safety goggles
  5. Gloves
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15
Q

What are the three major parts of a microscope?

A

The lens system, the adjustable light source, and the focusing mechanism.

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16
Q

Biological research is _________?

A

The study of living cells and organisms

17
Q

In a compound light microscope, what does the base do?

A

Supports the microscope and it, most of the time, contains a light switch, the light source and the voltage adjustment controls.

18
Q

Light from the light source passes through the condenser which?

A

Which focuses the light specimen on the slide supported on the microscope stage.

19
Q

What allows a viewer to increase or decrease the contrast in a compound microscope?

A

The iris diaphragm (which is also built into the condenser)

20
Q

By adjusting the ___________ _______ ________ you can move the _____________ _______ (and the attached slide) around allowing different parts of the slide to be illuminated. (Fill in the blanks)

A

Mechanical stage control/mechanical stage

21
Q

Microscopes usually have several _________ lenses attached onto a ____________ ___________ which allows objective lenses of different magnification power to be used on a sample. (Fill in the blanks)

A

objective/revolving nosepiece

22
Q
A