Lab Exam Flashcards
Bacterial growth media that contain specific ingredients to allow one to distinguish selected species of bacteria visual observation
Differential media
Are used for growth of only selected organisms and inhibit the growth of other organisms
Selective
Supports the growth of wide variety bacteria
General purpose media
Differential
Blood agar
EMB
Differential and selective
Tsa
Non selective general purpose
NA
General purpose
Mconcey
Selective and differential
Sim
Differential
Simmon citrate
Differential
Is green turns blue when positive
Simon citrate
Positive test indicates from pink to red at the top of the broth medium
Sim
Yellow indicates negative indole test
Sim
Gram negative turns pink
Mcconkey
Msa
Selective differential
Witch antimocrobic agent produced the largest average inhibition zone diameter
Tetracycline
Antibiotics broad spectrum
Penecilin
Erethomycin
Neomycin
Tetracycline
Witch bacteria was resistant to the antibiotics used
Aeruginosa
Which bacteria was the most sensitive
S auerous
What media was used for bacteria and antibiotics
Muiller Hinton 11 agar
Msa content medium
Msa contains salt
Sugar Manitol
If it can ferment manitol a acidic byproduct is made that causes phenol red to yellow
What is the ph indicator in msa
Phenol red
What bacteria grows on msa
Staphococolus areus
What bacteria turns msa yellow
Stephococlus Aerus
What enzyme activity does hydrogen peroxide test
Catalese
Used to determine hydrogen sulfide production indole production and motility
What is sim medium
What is sim used to detect in bacteria
Any blacking of medium indicates reduction of sulfur and is a positive result
What is a catalase test
The culture will bubble of oxygen present if no bubbling no reaction
Used for rapid identification of Enterobacter 12 different types of media and 15 bichemical tests
Enterotube