Lab Exam

Question 1

What do you need to bring to every lab period?

Answer 1

1. A lab coat
2. Lab manual
3. Standard note taking supplies
4. Full length pants or skirt
5. Eye protection
6. Waterproof felt pen
7. Time piece capable of measuring seconds
8. An elastic (for long hair)
9. Closed footwear

Question 2

Pure Culture

Answer 2

An isolated group of genetically identical cells

Question 3

Colony

Answer 3

A small mass of genetically identical cells

Question 4

Medium (pl. media)

Answer 4

a solution or solid that contains nutrients a cell can use to grow and divide. It can be left as a liquid or can be mixed with a substance to solidify it

Question 5

Lawn

Answer 5

a dense, even layer of bacteria on the plate with clearings where the antibiotics kill the cells

Question 6

Lawn

Answer 6

a dense, even layer of bacteria on the plate with clearings where the antibiotics kill the cells

Question 7

Light microscope

Answer 7

A microscope where light travels from the light source, shines on the specimen, and its image is then magnified by the lenses

Question 8

Ocular lens

Answer 8

Magnifies an image and inverts it for viewing

Question 9

Objective lens

Answer 9

Magnifies the image of the specimen

Question 10

Condenser lens

Answer 10

Focuses light on the specimen

Question 11

Condenser lens

Answer 11

Focuses light on the specimen

Question 12

Light source

Answer 12

Small powerful light bulb

Question 13

Field iris diaphragm

Answer 13

Controls the amount of light entering the lens system. Decreasing the amount of light can improve contrast

Question 14

Contrast

Answer 14

the difference between light and dark on the slide

Question 15

Stage

Answer 15

the horizontal surface where the slide is placed. It moves horizontally so you can locate an object on the slide, and vertically so you can focus on the slide

Question 16

Objective lenses

Answer 16

gather light from the specimen, magnify the image, and project the magnified image into the body tube

Question 17

Course focus knob

Answer 17

moves the stage up and down to bring an object into focus

Question 18

Fine focus knob

Answer 18

is the small knob on the course adjustment knob. Brings the object into focus under low power and is used for all focusing when using higher power lenses

Question 19

Body tube

Answer 19

contains a prism that allows light to pass from the objective lens to the ocular lens

Question 20

Ocular lens

Answer 20

magnifies the image

Question 21

Ocular lens

Answer 21

magnifies the image

Question 22

Total magnification of a microscope

Answer 22

The product of the magnification of the objective lens and the ocular lens

Total magnification=
magnification (ocular lens) x magnification (objective lens)

Question 23

Total magnification of a microscope

Answer 23

The product of the magnification of the objective lens and the ocular lens

Total magnification=
magnification (ocular lens) x magnification (objective lens)

Question 24

Osmosis

Answer 24

Passive transport where water moves across a membrane from an area of low solute concentration to an area of high solute concentration

Question 25

Hypotonic solution

Answer 25

has a lower solute concentration than inside the cell; cell is swollen (turgid)

Question 26

Isotonic

Answer 26

Cell is normal

Question 27

Hypertonic

Answer 27

Cell is shrinking