lab exam Flashcards
what is Kohler illumination?
a technique that produces even, glare-free illumination of a specimen in a microscope.
formula for dilution
volume of sample/total volume
what is serial dilution?
solution that is used to dilute a sample
what is included in a graphing chart?
axis title/graph title, scaled axis, and plotted data
when can you use a p-1000
201-1000 uL
when can u use a p-200
21-200 uL
micropipetting: when can you use a p-20?
1-20 uL
how do you use the microscope?
use the coarse adjustment knob at the 4x lens to focus your specimen and set diagram to 70-80%
What is the spectrophotometry machine’s purpose?
to measure the amount of light absorbed/transmitted by a substance at different wavelengths.
Total dilution factor
product of the dilution factors of each specieman
What are the parts of a microscope?
eyepiece, objective lens stage, light intensity, y-axis, x axis, and fine focus knob.
FOV calculations
FN objective/objective magnification
Total maginification
4x, 40x, 100x, and 400x
germ theory
the medical theory that specific microscopic organisms, or germs, cause specific diseases
koch’s postulates
4 criteria’s that establish whether a microorganism is the cause
