Lab Exam Flashcards
Forensic Entomology
Study of insects and other arthropods in forensic contexts
3 general areas in which forensic entomologists practice
Stored-product entomology, urban entomology, medicolegal entomology
Medicolegal entomology
Study, analysis, and interpretation of insects associated with the corpse
PMI
Post-mortem interval, time since death
Use of insects in medicolegal entomology
PMI, areas of wounds, movement of body after death
Why is forensic entomology regional in nature?
Insect growth and development relies on biochemical processes that depend on moisture/humidity and temperature, as well as environmental data
Where is the insect pinned on the pinning block?
Top step
Where is the first label pinned on the pinning block?
Second step from top
What is on the first insect specimen label?
Date of collection, locality, name of collector, identification of insect
Where is the second label pinned on the pinning block?
Bottom step (3rd from top)
What goes on the second insect specimen label?
Method of collection, food source if raised in lab, area of body it was collected from
When is the det label added?
Last
Det Label
Determination label, produced by professional insect taxonomist that verifies genus and species
How are immature/soft bodied insects labelled?
One on the outside of the label and one on the inside in case the outer one falls off and to transfer the inside one with the contents
How are immature/soft bodied insects stored?
In a vial with 70-80% ethanol
What goes on immature/soft bodied insect vial labels?
Date of collection, locality, name of collector, identification of insect (if possible). Can include area of body, length of maggot when collected, and if maggot was blanched in boiling water to keep from shrinking in alcohol
Necrophagous Insects
Attracted to and feed on the carrion
Omnivorous Insects
May be attracted to the carrion as a food source for their larval stages, adults may feed on other insects present
Predaceous Insects
Attracted to the carrion due to the large numbers of other insects present to feed on
Adventitious Insects
Not necessarily attracted to the carrion at all but may be found in association with it if it is in their habitat
Diptera Pinning Position
Through centre of thorax
Coleoptera Pinning Position
Through upper right elytra and abdomen
Hymenoptera Pinning Position
Through centre of thorax
Hemiptera Pinning Position
Through right hand thorax
Odonata Pinning Position
Through centre of thorax
Beetle vs Fly Larvae
Beetles have prolegs, flies do not
Locard’s Principle
Whenever there is contact between two objects/people/etc., there is an exchange of material
How to Examine Leaf Fragments
Margin/teeth type, venation (primary, secondary, and tertiary)
Why is the underside used in an epidermal leaf peel?
So you can obtain an impression of the stomata pattern and venation of the epidermis
How do the acetone and acetate work together in a leaf peel?
Acetone adheres the acetate to the leaf. The acetone soften/melts the acetate and allows it to conform to the epidermal pattern of the leaf
How can you tell the difference between a leaf and a leaflet?
Leaf has a bud at the base and leaflet does not. Leaf has circular or semicircular base to surround the bud but leaflet does not.
Which tree is an exception to the leaf vs leaflet rules?
Sycamore tree has hollow petiole that encloses next year’s bud
Alternate Leaf Attachment
One leaf at each node (every other on each side)
Opposite Leaf Attachment
Two leaves at each node (one on each side at each spot)
Whorled Leaf Attachment
3 or more leaves at each node
Decussate Leaf Attachment
Each leaf attached at 90 degrees (not 180) from those above and below (opposite or alternate, but switches directions at each)
Aerial Net
A fine mesh net used to catch flying insects
Pan Trap
Plastic pan that can be counter sunk into the ground, 1/2 full of water/salt/soap mixture, to catch crawling and flying insects. Often yellow to attract dipterans and hymenopterans.
Pitfall Trap
Cup counter sunk in ground to catch crawling insects. A funnel on top to prevent them from getting out. 1/2 full of water/salt/soap/EtOH mixture.
Window Trap
Large piece of glass/plexiglass or even an old window suspended between trees with an eavestrough attached to bottom with water/salt/soap/EtOH mixture. Fly into window, stunned and fall into trap and are killed.
Aspirator
An arrangement of tubes and stoppers allowing small flying insects in particular to be sucked into a holding tube by the collector, using their own inhalation; screens or cotton gauze prevent inhalation of insect by collector.
Killing Jar
Jar with a screw cap and a wick or plaster of Paris that are soaked in ethyl acetate to kill insects when closed in the jar.
Insect Pins
Specialty coated straight pins that will not rust due to insect fluids
Pinning Block
A stepped block with a hole drilled in each step which will allow the appropriate spacing of insect and labels on a pin
Spreading Board
Used to mount moths, butterflies, dragon flies, or anything with large wings that are to be mounted in an open position
How to Use Spreading Board
Wings are spread on the wooden side platforms while the body of the insect is pinned in the groove down the middle of the board.
Pinning with Points
Insects too small to pin are glued to the point of a small triangular or tear dropped shape of archival quality card stock, and the mounting pin is placed through the wide end of the point.
Blow Fly vs Flesh Fly Maggots
Blow fly spiracles on surface, flesh fly spiracles are depressed
Calliphoridae
Blow Fly
Sarcophagidae
Flesh Fly
Admedial (Botany)
Toward the midline of the lamina
Apex (Botany)
Usually the upper ~25% of the lamina
Apical (Botany)
Toward the apex of the leaf
Basal (Botany)
Toward the base of the leaf
Base (Botany)
Usually the lower ~25% of the lamina
Concave (Botany)
Curving toward the center of the lamina or tooth
Convex (Botany)
Curving away from the center of the lamina or tooth
Costal Vein (Botany)
Primary and secondary veins that extend from the base of the leaf or from a primary
toward the leaf margin
Exmedial (Botany)
Away from the midline of the lamina
Intercoastal Area (Botany)
The region bounded by two costal veins
Lamina (Botany)
Blade of leaf. The expanded, flat part of a leaf or leaflet
Margin (Botany)
Edge of the lamina/blade
Midvein
Medial primary vein, in pinnate leaves this is the only primary
Node (Botany)
The place where a leaf is (or was) attached to the axis (stem)
Petiole
The stalk of a leaf
Petiolule
The stalk of a leaflet in a compound leaf
Primary Vein (Botany)
The widest vein of the leaf and any others of like width and/or course, usually originate at or just above the petiole
Rachis
The prolongation of the petiole of a pinnately compound leaf upon which leaflets are attached
Secondary Vein (Botany)
The next narrower class of veins after the primary, originating from the primary or primaries
Sessile
A leaf or leaflet that is lacking a petiole or petiolule
Tertiary Vein (Botany)
The next narrower class of veins after the secondaries, originating from the secondaries or primaries
Vein Course (Botany)
Path of the vein on the leaf
Munsell Colour System
Series of colour charts for soil horizon colour classification
Munsell Hue
Colours. Range from red to yellow
Munsell 10Y
Very yellow
Munsell 10R
Very red
Munsell 5YR
Half way between yellow and red
Munsell 7YR
More yellow than red
Munsell 2 YR
More red than yellow
Munsell Value
Scale from white to black, brightness of the colour
Munsell Chroma
Intensity of the colour, scale from grey to vibrant
What does Munsell 5YR:3/4 mean?
5YR: Hue
3: Value
4: Chroma
How are the Munsell colours written?
Hue: Value/Chroma
What is the soil profile of a podzol from top to bottom?
F1, F2, A1, A2, B1, B2, C
Soil Profile Top to Bottom
O layer (or F), A layer, B layer, C layer
F1 Soil Layer
Top litter layer, nothing broken down, whole leaf shapes, some fermentation, fungus
F2 Soil Layer
More decomposition but still littery, pine needle layer
A1 Soil Layer
Darker due to humus leaching down from F2
A2 Soil Layer
Elluvial, iron leached down and out, ashy colour
B1 Soil Layer
Illuvial, iron has washed in from above, orange brown from iron
B2 Soil Layer
Less orange brown
C Soil Layer
Unweathered and parent material
What type of soil is in Sudbury?
Podzol, or podzolic soil
What is podzol?
Hydromorphic soil, meaning there is a downward movement of water and ions and consequent leaching of constituents
How do you see soil profiles?
Core method or dig a pit with nice crisp vertical sides
Spheroidal Peds
Less than 1 cm diameter, granular or crumb
Granular vs Crumb Peds
Both small and spheroidal but granular is solid/hard/can’t crush easily, crumb can squish easily
Platy/Plate-Like Peds
In kaolin (non swelling clay), cracking in horizontal plane. Usually in subsoil, sometimes the result of particles leading down.