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MICRO-PARA LEC > LAB EQUIPMENTS > Flashcards

LAB EQUIPMENTS Flashcards

1
Q

uses a single lens to view objects

A

SIMPLE MICROSCOPE

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2
Q

uses light to view objects

A

COMPOUND MICROSCOPE

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3
Q

Composition of compound microscope

A

Oculars or Eyepiece
Objectives

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4
Q

Types of compound microscope

A

A. Bright-field Microscope
B.Dark-field Microscope
C.Flouresence Microscope
D.Phase-contrast Microscope

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5
Q

most commonly used type of
microscope in routine microscopy

A

Bright-field Microscope

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6
Q

produce a dark image on a bright
background

A

Bright-field Microscope

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7
Q

uses transmitted light to observe
targets at high magnification

A

Bright-field Microscope

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8
Q

a brightfield microscope that is
arranged so that the light source is
blocked off, causing light to scatter
as it hits the specimen

A

Dark-field Microscope

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9
Q

produces a bright image on a dark
background

A

Dark-field Microscope

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10
Q

Coupling compound that bind to certain cellular components

A

Flourescein

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11
Q

biological microscope that
observes fluorescence emitted by
samples by using special light
sources such as mercury lamps

A

Flouresence Microscope

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12
Q

allows coupling compounds such
as fluorescein to bind to certain
cellular components

A

Flouresence Microscope

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13
Q

visualizes minute surface
irregularities by using light
interference

A

Phase-contrast Microscope

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14
Q

commonly used to observe living
cells without staining them

A

Phase-contrast Microscope

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15
Q

ability of a microscope to produce an image of an object at a scale
larger than its actual size

A

Magnification

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16
Q

combination of the eyepieces and the
objective lens

A

Total Magnification

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17
Q

Ocular lens magnification =

A

10x

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18
Q

4 objective lenses

A

A. scanning
B. low power field
C. high power field
D. Oil immersion

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19
Q

scanning =

A

4x

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20
Q

low power field =

A

10x

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21
Q

high power field =

A

40x

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22
Q

Oil immersion =

A

100x

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23
Q

Total Magnification formula

A

Total Magnification = Ocular lens x Objective lens

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24
Q

ability of a microscope to distinguish details
of a specimen or sample

A

Resolution/ Resolving Power

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25
Q

▪︎ wavelength
▪︎ numerical aperture

A

Resolution/Resolving Power

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26
Q

ability to gather
light and to resolve fine specimen detail
while working at a fixed object (or
specimen) distance

A

numerical aperture

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27
Q

Also called cell culture dish

A

Petri Dish

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28
Q

named after its inventor, German
bacteriologist, Julius Richard Petri

A

Petri Dish

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29
Q

a shallow transparent covered dish used
to culture cells, such as bacteria or fungi

A

Petri Dish

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30
Q

Has a transparent body allow researchers to
constantly monitor contents

A

Culture Tubes

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31
Q

shaped for easier retrieval and minimal
sample loss

A

Culture Tubes

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32
Q

extremely resistant to accommodate any
operation or harsh sample

A

Culture Tubes

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33
Q

ability of a microscope to stay relatively in
focus as the user switches among the
objectives

A

Parfocal

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34
Q

Scanning + course adjustment

A

Parfocal

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35
Q

also called a smear loop, inoculation
wand, microstreaker, wire loop

A

Inoculating loop

36
Q

consists of a thin handle with a loop
about 5 mm wide or smaller at the end

A

Inoculating Loop

37
Q

used to pick up and transfer a small
sample (inoculum) from a culture of
microorganisms into a petri dish

A

Inoculating Loop

38
Q

streaking technique

A

Inoculating Loop

39
Q

are used when fingers are too
large to grasp small objects

A

Forceps

40
Q

when many objects need to be
held at one time

A

Forceps

41
Q

in Bacteriology lab,used for
handling glass slides during the
staining process

A

Forceps

42
Q

holds multiple microscopic
slides simultaneously for
staining process

A

Staining Rack

43
Q

used to transfer and inoculate living
microorganisms

A

Inoculating Needle

44
Q

made from nichrome or platinum
wire affixed to a metallic handle

A

Inoculating Needle

45
Q

base of the needle is dulled, resulting in
a blunted end

A

Inoculating Needle

46
Q

stabbing technique

A

Inoculating Needle

47
Q

used for steaming or heating
process tubes, flasks or beakers

A

Water Bath

48
Q

used to hold specimen viewed
under the microscope

A

Glass slides | Cover slips

49
Q

device used to measure the distance
between two opposite sides of an
object (usually diameter)

A

Vernier Caliper

50
Q

In antibiotic susceptibility testing:
• to measure the zone of inhibition

A

Vernier Caliper

51
Q

candle flame will consume most
of O2
in the jar & will produce an
elevated level of CO2

A

Candle Jar

52
Q

these conditions are ideal for
growth of microaerophilic
organisms

A

Candle Jar

53
Q

Filter paper disks impregnated with
standardized concentration of an
antimicrobial agent

A

Antibiotic Discs

54
Q

Used in susceptibility testing

A

Antibiotic Discs

55
Q

used to grow microorganism
in optimum temperatures

A

Incubator

56
Q

usual temperature of incubation
is 35-37°C

A

Incubator

57
Q

maintains a constant
temperature specifically
suitable for growth of a
specific microbe

A

Incubator

58
Q

“Steam under pressure”

A

Autoclave

59
Q

used to sterilize liquid substances such as prepared media,
solutions, glasswares

A

Autoclave

60
Q

water is boiled in a closed containerto reach 100°C

A

Autoclave

61
Q

steambuilds up inside the autoclave increasing the
temperature much beyond 100°C

A

Autoclave

62
Q

pressure reaches 15 pounds/in2 or 15 psi

A

Autoclave

63
Q

temperature reaches 121°c

A

Autoclave

64
Q

Sterility time of autoclave

A

15 minutes

65
Q

high temperature is required to kill all bacteria including
heat resistantspore-formers

A

Autoclave

66
Q

Air curtain prevent organism to go outside,if
the glass panel is closed (UV would turn on) and vice versa

A

Glass panel

67
Q

enclosed, ventilated laboratory workspace for safely
working with materials contaminated with (or
potentially contaminated with) pathogens requiring a
defined biosafety level

A

Biosafety Cabinet / Hood

68
Q

several different types of BSC exist, differentiated by the
degree of biocontainment required

A

Biosafety Cabinet / Hood

69
Q

Types of BSC cabinets

A

Class I
Class II
Class III

70
Q

Hepa filter is used to filter air before it is released

A

Class I

71
Q

Personnel and Environmental protection

A

Class I

72
Q

Personnel are protected by moving air away from them

A

Class I

73
Q

Product, personnel and environment protection

A

Class II

74
Q

Has four subtypes (A1, A2, B1, B2)

A

Class II

75
Q

High Risk biological agents

A

Class III

76
Q

Maximum protection

A

Class III

77
Q

Known as “glove box”

A

Class III

78
Q

Any material passes through a double door – door autoclave

A

Class III

79
Q

food for the organism

A

Culture Medium

80
Q

provide similar environmental and
nutritional conditions that exist in
the natural habitat of a bacterium

A

Culture Medium

81
Q

Contains water, source of carbon
and energy, source of nitrogen,
trace elements and some growth
factors

A

Culture Medium

82
Q

Types of Culture Medium

A
  1. Enriched media
  2. Enrichment media
  3. Selective media
  4. Differential media
83
Q

used to grow micro-organisms of a wide variety
contains high nutrition in form of egg yolk or blood serum so on

A

Enriched Media

84
Q

selective medium used to grow specific microogranism
prevent contaminating microbes from growing

A

Enrichment media

85
Q

Solid media

A

Selective media

86
Q

differentiate kinds of bacteria

A

Differential media

MICRO-PARA LEC (2 decks)

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