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Lab Dx Mycology Flashcards

0
Q

It is recommended that specimens should be stored under refrigeration at what temperature?

A

4degreesC

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1
Q

All specimens should be collected _______ and placed in sterile containers.

A

aseptically

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2
Q

This should be stored at 30-37degreesC

A

Blood and CSF

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3
Q

This should be stored at 15-30degreesC

A

Dermatologic specimens

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4
Q

Collection notes for BLOOD

A

Wright’s or Giemsa stain bottles

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5
Q

Isolation media for BLOOD

A

Sodium polyethanol sulfonate (SPS)

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6
Q

Collection notes for BONE MARROW

A

Heparin as anticoagulant and collect 0.5 ml of marrow

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7
Q

Isolation media for BONE MARROW

A

BHI broth, SDA

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8
Q

Collection notes for CSF

A

> 2ml: centrifugation

<2ml: uncentrifuged specimen

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9
Q

Isolation media for CSF

A

SDA, BHI with blood

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10
Q

Collection notes for HAIR

A

Hairs that fluoresce or are broken and scaly

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11
Q

Isolation media for HAIR

A

SDA, SDA-CC

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12
Q

Collection notes for NAILS

A

Scrape dissolved or hyperkeratotic areas

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13
Q

Isolation media for NAILS

A

SDA, SDA-CC

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14
Q

Collection notes for SKIN

A

Scrape outer edge of ring in ringworm infections

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15
Q

Isolation media for SKIN

A

SDA, SDA-CC, SDA with oil for Malassezia

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16
Q

Collection notes for MUCOCUTANEOUS

A

Scraping plaque with tongue depressor if Candida is suspected

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17
Q

Isolation media for MUCOCUTANEOUS

A

SDA, SDA-CC

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18
Q

Collection notes for SUBCU TISSUE, LESIONS AND ABSCESSES

A

Biopsy or needle aspiration and examine presence of granules

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19
Q

Isolation media for SUBCU TISSUE, LESIONS AND ABSCESSES

A

SDA, SDA-CC, anaerobic BHI with blood

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20
Q

Collection notes for SPUTUM, BRONCHIAL WASHINGS AND TRANSTRACHEAL ASPIRATES

A

Early morning sputum for 3 consecutive days

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21
Q

Isolation media for SPUTUM, BRONCHIAL WASHINGS AND TRANSTRACHEAL ASPIRATES

A

SDA, BHI with blood and CC, anaerobic BHI with blood

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22
Q

Collection notes for THROAT

A

2 sterile swabs

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23
Q

Isolation media for THROAT

A

SDA, SDA-CC

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24
Q

Collection notes for URINE

A

Midstream clean catch and detect fungal elements

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25
Q

Isolation media for URINE

A

SDA, SDA-CC

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26
Q

Collection notes for VAGINAL, CERVICAL

A

2 swabs examine with KOH

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27
Q

Isolation media for VAGINAL, CERVICAL

A

SDA, SDA-CC

28
Q

Source of culture media

A

Nitrogen and carbon

29
Q

What does fungi prefer for its growth?

A

Moisture and increased humidity

30
Q

How many weeks should a culture be incubated?

31
Q

Yeasts grow within _______ while H. capsulatum require _________ weeks to grow

A

24 to 72 hours… 10-12

32
Q

Give the four Primary Isolation Media

A

SDA, BHI, DTM, Mycosel/Mycobiotic Agar

33
Q

General isolation medium and it contains peptone and glucose

A

Sabouraud’s Dextrose Agar (SDA)

34
Q

Primary recovery of saprobic and pathogenic fungi

35
Q

Isolation of saprophytic and pathogenic fungi from sterile sites especially SYSTEMIC MYCOSES

36
Q

Useful for pathogenic fungi exclusive for dermatophytes

37
Q

For the recovery of fungi from BLOOD and BONE MARROW

A

BHI Biphasic Culture Bottles

38
Q

Isolation of dermatophytes from hair, skin and nail specimens

39
Q

Indicator that it has changed to alkaline pH

A

Yellow to red

40
Q

Inhibitory agents: cycloheximide and chloramphenicol. This is similar to DTM

A

Mycosel/Mycobiotic Agar

41
Q

This is a differential culture media wherein it shows stimulation of conidiation and chlamydospore production in Candida spp

A

Cornmeal Agar with Tween 80

42
Q

Useful for spp ID of Candida

A

Cornmeal Agar with Tween 80

43
Q

Cornmeal agar: numerous chlamydospores with flight clusters of blastoconidia

A

C. albicans

44
Q

Cornmeal agar: fewer blastoconidia and no chlamydospores present

A

C. tropicalis

45
Q

Cornmeal agar: colonies appear as “spiders”

A

C. parasilopsis

46
Q

Cornmeal agar: elongated blastoconidia in a “log in a stream” arrangement

A

C. pseudotropicalis

47
Q

Differential culture media for the isolation of C. neoformans

A

Birdseed/Niger seed Agar

48
Q

This production breaks down the medium resulting to melanin forming black brown colonies

A

Phenol Oxidase

49
Q

Differential culture media useful in slide cultures

A

Potato Dextrose Agar

50
Q

Differential culture media which demonstrates pigment production of T. rubrum

51
Q

Differential culture media for the detection of Urease producrion by C. neoformans

52
Q

Differenti culture media which differentiates T. rubrum from T. mentagrophytes

53
Q

They can grow as white patches with glossy surface

54
Q

They often appear whitish grey with fuzzy edges

55
Q

Used in screening for keratinized tissue

56
Q

Most accurate method to preserve and observe fungal microstructure

A

Slide culture

57
Q

Slide culture should not be performed on ______, _______, and ________.

A

Coccidioides, Histoplasma, Blastomyces

58
Q

To identify the capsule of C. neoformans

59
Q

Used to visualize microscopic fungal morphology by imparting a blue color to the cell walls

60
Q

A brightening agent can bind to the chitin in the fungal cell wall providing contrast when examined under fluorescence xscope

A

Calcofluor white

61
Q

Used for the detection of Intracellular Histoplasma capsulatum; C. neoformans also stains quite well in this mtd

A

Giemsa or Wright’s stain

62
Q

Used to screen fungi in histologic spx; fungi is outlined black against pale blue background

A

Methenamine-Silver Nitrate Stain

63
Q

Stains hyphae of molds and some yeasts

64
Q

Hydrolyzes CHO of cell walls of the org to form aldehydes and then react w fuchsin dye to form pink-purple complex

A

Periodic Acid

65
Q

Differential test specific for C. albicans

A

Germ tube test

66
Q

Differential test for specie ID of some yeasts; INOSITOL:C. Neoformans vs Rhodotorula

A

CHO Assimilation Test for Yeast

67
Q

Differential test specific for Cryptococcus spp

A

Rapid Urease Test

Mycology-Virology (1 decks)

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