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Viro > Lab Diagnosis > Flashcards

Lab Diagnosis Flashcards

1
Q

Viral shedding is greatest when?

A

During Early stages of infection

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2
Q

Viral culture results can decrease rapidly inhow many days

A

3

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3
Q

These can be added to prevent tissues from dryinh

A

Transport media
Saline
Trypticase soy broth

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4
Q

Buffered transport media contain proteins go protect less stable viruses

A

Albumin
Gelatin
Serum

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5
Q

Thepresence of this will disrupt the host cell and results to loss of viral viability

A

Ice crystals

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6
Q

What spx can be used to isolate eneteroviruses after the cessation of respiratory shedding

A

Stool

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7
Q

Major causes of aseptic meningitis

A

Enteroviruses

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8
Q

4 major methods in to diagnose viral infections

A

Direct detection
Nucleic acid based detection
Cell cultures
Serologic assays

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9
Q

Not as sensitive as cell culture but can offer quick results for rapid therapy

A

Direct detection

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10
Q

Bright field microscopy can be used to what virus

A

Poxvirus

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11
Q

Type of microscopy that is useful to detect non culturable virus

A

Electron microscope

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12
Q

It is the distinctive and characteristic visual changes in infected cells

A

Cytopathic effects

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13
Q

CPE can be detected in _____ using bright field

A

Cell scrapings

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14
Q

Smear that detect cowdry type A bodies

A

Tzanck smear

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15
Q

Owl eyes is seen in what virus

A

HSV AND VZV

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16
Q

_________ smear reveal HPV

A

Papanicolaou

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17
Q

Squamous cells with enlarged nucleus and non staining halo (perinuclear halo)

A

Koilocytes

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18
Q

Eosinophilic cytoplasmic inclusions in neurons

A

Negro bodies

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19
Q

Negro bodies are seen in

A

Rabies

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20
Q 
EIA using multiwell microtiter plates can detect what virus in 
Respiratory spx 
Serum or plasma 
Stool 
Cutaneous lesions and conjuctival
A

RSV and influenza A
Hep b virus and HIV -1
Enteric adenovirus
HSV

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21
Q

EIA in single test platforms detects these viruses in
Respiratory spx
Rectal swabs
Serum

A

RSV, influenzavirus A and B
Rotavirus and enteric adenovirus
West nile fever virus

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22
Q

EIA negative results are confirmed with

A

Cell culture or IF or nucleic acid based test

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23
Q

This test gives a quantitative result

A

Nucleic acid based detection

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24
Q

Combination of flow cytometry and PCR for mutiplex detectio

A

luminex system

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25
Q

Nucleic acid based assay that primarily detects blood pathogens

A

Gene amplification technique

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26
Q

Rapid subtyping for influenza A virus

A

Microarray assay

27
Q

Luminex system detects how many type or subtype pathogens and wihtin how many hrs

A

20

5 hrs

28
Q

The gold standard

A

Viral isolation

29
Q

Three methods for viral isolation

A

Cell culture
Animal inoculation
Embryonated eggs

30
Q

Most commonly used isolation method

A

Cell culture

31
Q

Coxsackie virus require what animal for isolation

A

Suckling mice

32
Q

Isolation of Influenza virus is enhanced in

A

Embryonated eggs

33
Q

Most of the clinical workload focuses on the detection of

A

HSV and respiratory viruses

34
Q

Common clinical viruses can be identified within

A

48 hrs

35
Q

Common clinical viruses

A

HIPRAE

HSV 
Influenza virus A ANDB 
PIV 1 - 4 
RSV 
Adenovirus 
Enterovirus
36
Q

Used to indicate culture of cells in vitro

A

Cell culture

37
Q

Denote the growth of tissues or organ so that the architecture or function is reserved

A

Tissue culture

38
Q

Cell culture that is obtained from tissue removed from an animal and has minimal cell division

A

Primary cell culture

39
Q

Tissue in promary cell culture is treated with what enzyme to disperse individual cell further

A

Trypsin

40
Q

Process of removing cells from the surface, diluting and placing them in a new container

A

Splitting or passaging

41
Q

Commonly used primary cell culture

A

Primary monkey kidney cells

42
Q

Can divide but has limited passage ( up to50 gen)

A

Finite cell cultures

43
Q

Term for having two copies of chromosome

A

Diploid

44
Q

True or false

As the number of passae increases, cells become more insensitive to viral infection

A

True

45
Q

Standard finite cell culture

A

Human neonatal lung

46
Q

Cell culture capable of infinite passage and are heteroploid

A

Continuous cell cultures

47
Q

HEp2 is derived from

A

Human laryngeal epithelial carcinoma

48
Q

A549 is dervied from

A

Human lung carcinoma

49
Q

Vero is derived from

A

Monkey kidney

50
Q

Developed from combining two cell lines

A

Mixed cell culture

51
Q

Exhibits rapid growth, plaque formation, And growth ondifferent cell types

A

HSV

52
Q

HSV produces CPE within

A

24 hrs

53
Q

Growth on rabbit kidney cells is indicative of what

A

HSV

54
Q

Produces HSV-like CPE and growns only on diploid fibroblast

A

CMV

55
Q

Adenovirus CPE appearance

A

Cluster of grapes

56
Q

Produce classic syncytial formation

A

RSV

57
Q

Gian multinucleated cells resulting from cell fusion as a consequence of viral infection

A

Syncytia

58
Q

Cells infected with influenza virus expresses what which binds to RBC

A

Hemagglutinin protein

59
Q

True or false
(Hemadsorption)
If positive, the rbc will stick or adsorb to the infected cell

A

True

60
Q

Stain used to detect viral antigen and is used to screen cultures before final negative result

A

Fluorescent antibody stain

61
Q

Can identify more rapidly vrisuses than xian

A

Centrifugation-enhances shell vial culture

62
Q

Sa small round flat bottmed tube with screw cap

A

Shell vial

63
Q

Modifcation of centrifugation-enhanced shell vial culture

A

Use of flat bottomed microtiter plates

64
Q

Measures the host response rather than the directly detecting the virus

A

Serologic assays

Viro (2 decks)

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