Lab Diagnosis

Question 1

Viral shedding is greatest when?

Answer 1

During Early stages of infection

Question 2

Viral culture results can decrease rapidly inhow many days

Answer 2

3

Question 3

These can be added to prevent tissues from dryinh

Answer 3

Transport media
Saline
Trypticase soy broth

Question 4

Buffered transport media contain proteins go protect less stable viruses

Answer 4

Albumin
Gelatin
Serum

Question 5

Thepresence of this will disrupt the host cell and results to loss of viral viability

Answer 5

Ice crystals

Question 6

What spx can be used to isolate eneteroviruses after the cessation of respiratory shedding

Answer 6

Stool

Question 7

Major causes of aseptic meningitis

Answer 7

Enteroviruses

Question 8

4 major methods in to diagnose viral infections

Answer 8

Direct detection
Nucleic acid based detection
Cell cultures
Serologic assays

Question 9

Not as sensitive as cell culture but can offer quick results for rapid therapy

Answer 9

Direct detection

Question 10

Bright field microscopy can be used to what virus

Answer 10

Poxvirus

Question 11

Type of microscopy that is useful to detect non culturable virus

Answer 11

Electron microscope

Question 12

It is the distinctive and characteristic visual changes in infected cells

Answer 12

Cytopathic effects

Question 13

CPE can be detected in _____ using bright field

Answer 13

Cell scrapings

Question 14

Smear that detect cowdry type A bodies

Answer 14

Tzanck smear

Question 15

Owl eyes is seen in what virus

Answer 15

HSV AND VZV

Question 16

_________ smear reveal HPV

Answer 16

Papanicolaou

Question 17

Squamous cells with enlarged nucleus and non staining halo (perinuclear halo)

Answer 17

Koilocytes

Question 18

Eosinophilic cytoplasmic inclusions in neurons

Answer 18

Negro bodies

Question 19

Negro bodies are seen in

Answer 19

Rabies

Question 20

EIA using multiwell microtiter plates can detect what virus in 
Respiratory spx 
Serum or plasma 
Stool 
Cutaneous lesions and conjuctival

Answer 20

RSV and influenza A
Hep b virus and HIV -1
Enteric adenovirus
HSV

Question 21

EIA in single test platforms detects these viruses in
Respiratory spx
Rectal swabs
Serum

Answer 21

RSV, influenzavirus A and B
Rotavirus and enteric adenovirus
West nile fever virus

Question 22

EIA negative results are confirmed with

Answer 22

Cell culture or IF or nucleic acid based test

Question 23

This test gives a quantitative result

Answer 23

Nucleic acid based detection

Question 24

Combination of flow cytometry and PCR for mutiplex detectio

Answer 24

luminex system

Question 25

Nucleic acid based assay that primarily detects blood pathogens

Answer 25

Gene amplification technique

Question 26

Rapid subtyping for influenza A virus

Answer 26

Microarray assay

Question 27

Luminex system detects how many type or subtype pathogens and wihtin how many hrs

Answer 27

20 | 5 hrs

Question 28

The gold standard

Answer 28

Viral isolation

Question 29

Three methods for viral isolation

Answer 29

Cell culture Animal inoculation Embryonated eggs

Question 30

Most commonly used isolation method

Answer 30

Cell culture

Question 31

Coxsackie virus require what animal for isolation

Answer 31

Suckling mice

Question 32

Isolation of Influenza virus is enhanced in

Answer 32

Embryonated eggs

Question 33

Most of the clinical workload focuses on the detection of

Answer 33

HSV and respiratory viruses

Question 34

Common clinical viruses can be identified within

Answer 34

48 hrs

Question 35

Common clinical viruses

Answer 35

HIPRAE ``` HSV Influenza virus A ANDB PIV 1 - 4 RSV Adenovirus Enterovirus ```

Question 36

Used to indicate culture of cells in vitro

Answer 36

Cell culture

Question 37

Denote the growth of tissues or organ so that the architecture or function is reserved

Answer 37

Tissue culture

Question 38

Cell culture that is obtained from tissue removed from an animal and has minimal cell division

Answer 38

Primary cell culture

Question 39

Tissue in promary cell culture is treated with what enzyme to disperse individual cell further

Answer 39

Trypsin

Question 40

Process of removing cells from the surface, diluting and placing them in a new container

Answer 40

Splitting or passaging

Question 41

Commonly used primary cell culture

Answer 41

Primary monkey kidney cells

Question 42

Can divide but has limited passage ( up to50 gen)

Answer 42

Finite cell cultures

Question 43

Term for having two copies of chromosome

Answer 43

Diploid

Question 44

True or false | As the number of passae increases, cells become more insensitive to viral infection

Answer 44

True

Question 45

Standard finite cell culture

Answer 45

Human neonatal lung

Question 46

Cell culture capable of infinite passage and are heteroploid

Answer 46

Continuous cell cultures

Question 47

HEp2 is derived from

Answer 47

Human laryngeal epithelial carcinoma

Question 48

A549 is dervied from

Answer 48

Human lung carcinoma

Question 49

Vero is derived from

Answer 49

Monkey kidney

Question 50

Developed from combining two cell lines

Answer 50

Mixed cell culture

Question 51

Exhibits rapid growth, plaque formation, And growth ondifferent cell types

Answer 51

HSV

Question 52

HSV produces CPE within

Answer 52

24 hrs

Question 53

Growth on rabbit kidney cells is indicative of what

Answer 53

HSV

Question 54

Produces HSV-like CPE and growns only on diploid fibroblast

Answer 54

CMV

Question 55

Adenovirus CPE appearance

Answer 55

Cluster of grapes

Question 56

Produce classic syncytial formation

Answer 56

RSV

Question 57

Gian multinucleated cells resulting from cell fusion as a consequence of viral infection

Answer 57

Syncytia

Question 58

Cells infected with influenza virus expresses what which binds to RBC

Answer 58

Hemagglutinin protein

Question 59

True or false (Hemadsorption) If positive, the rbc will stick or adsorb to the infected cell

Answer 59

True

Question 60

Stain used to detect viral antigen and is used to screen cultures before final negative result

Answer 60

Fluorescent antibody stain

Question 61

Can identify more rapidly vrisuses than xian

Answer 61

Centrifugation-enhances shell vial culture

Question 62

Sa small round flat bottmed tube with screw cap

Answer 62

Shell vial

Question 63

Modifcation of centrifugation-enhanced shell vial culture

Answer 63

Use of flat bottomed microtiter plates

Question 64

Measures the host response rather than the directly detecting the virus

Answer 64

Serologic assays