lab d

Question 1

What is the primary purpose of blood culture?

Answer 1

To detect and identify microorganisms in the bloodstream.

Question 2

What type of media is used in Castaneda’s biphasic blood culture method?

Answer 2

BHI broth and agar.

Question 3

True or False: Blood culture can only detect bacterial infections.

Answer 3

False.

Question 4

What does BHI stand for in BHI broth?

Answer 4

Brain Heart Infusion.

Question 5

Fill in the blank: Castaneda’s biphasic media consists of ______ and agar.

Answer 5

BHI broth.

Question 6

What is the significance of using biphasic media in blood cultures?

Answer 6

It allows for both aerobic and anaerobic growth of microorganisms.

Question 7

Multiple Choice: Which of the following is NOT a component of BHI broth? A) Beef extract B) Peptone C) Glucose D) Sodium chloride

Answer 7

D) Sodium chloride.

Question 8

What is the incubation temperature for blood cultures?

Answer 8

Typically 35-37 degrees Celsius.

Question 9

How long should blood cultures be incubated before considering them negative?

Answer 9

At least 5 to 7 days.

Question 10

True or False: The presence of bubbles in the broth indicates contamination.

Answer 10

True.

Question 11

What type of specimen is primarily used in blood cultures?

Answer 11

Whole blood.

Question 12

What is the role of agar in Castaneda’s biphasic media?

Answer 12

To provide a solid surface for colony growth.

Question 13

Fill in the blank: Blood culture is crucial for diagnosing ______ infections.

Answer 13

Systemic.

Question 14

What is the recommended volume of blood for adult blood cultures?

Answer 14

10 to 20 mL.

Question 15

Multiple Choice: Which of the following pathogens can be detected by blood cultures? A) Fungi B) Viruses C) Protozoa D) All of the above

Answer 15

D) All of the above.

Question 16

What is the purpose of subculturing from blood cultures?

Answer 16

To isolate and identify specific microorganisms.

Question 17

True or False: Anaerobic bacteria can be detected using Castaneda’s biphasic media.

Answer 17

True.

Question 18

What does a positive blood culture indicate?

Answer 18

The presence of infectious agents in the bloodstream.

Question 19

What is the recommended method for collecting blood specimens for culture?

Answer 19

Using sterile technique with venipuncture.

Question 20

Fill in the blank: Castaneda’s biphasic media is designed to enhance the growth of ______ organisms.

Answer 20

Fastidious.

Question 21

What is the first step after obtaining a blood specimen for culture?

Answer 21

Inoculate the media immediately.

Question 22

Multiple Choice: Which of the following is a common contaminant in blood cultures? A) Staphylococcus epidermidis B) Streptococcus pneumoniae C) Listeria monocytogenes D) Escherichia coli

Answer 22

A) Staphylococcus epidermidis.

Question 23

What is the main advantage of using biphasic media over traditional media?

Answer 23

Increased sensitivity for detecting a wider range of pathogens.

Question 24

True or False: Blood cultures should be performed only in cases of suspected septicemia.

Answer 24

False.

Question 25

What is the role of glucose in BHI broth?

Answer 25

To serve as a carbohydrate source for microorganisms.