Lab assesment

Question 1

What was the function of MnCl2

Answer 1

To clump the nucleic acids together so they can be removed from the solution to insure that there is no nucleic interference with the enzyme reading

Question 2

What was the function of Ammonium sulphate

Answer 2

To separate and purify the proteins in the crude extract. Done in a series of steps with an increase in concentration so the proteins become less soluble and the proteins precipitate out of the solution

Question 3

What was the function of EDTA

Answer 3

Binds the metal ions within the solution so they dont interfere with the enzymes in the solution

Question 4

What was the function of Methanol

Answer 4

To clump the proteins together by weakening the hydrophilic interactions which results in the proteins being precipitated out of solution

Question 5

What was the function of P-nitrophenol

Answer 5

Used to measure the enzymatic reaction and it is used to quantify acid phosphatase via colour change