Lab Apparatus Flashcards
Safety glasses
Protect eyes from chemical splashes, fire and biohazardous material.
Must be worn during ALL labs.
Gloves
Wear gloves whenever in lab and do not touch your hair and face with them.
~ Disinfect them with hand san itizer periodically during lab.
Do not leave the lab with your gloves.
Dispose the gloves after each lab
Serological pipettes
To accurate pipette relatively small volumes, typically 1-25 ml during experimentation.
-Individually wrapped, sterile pipettes are used when working with cell culture under aseptic conditions.
Micropipetes
Instrument used to measure and extract very small amounts of liquids from a solution.
• Different types have different levels of accuracy but usually to the nearest microliter
• Often used to handle small amounts of solutions typical in molecular biology.
Plastic micropipette tips
Disposable, autoclavable tips used to protect the micropipetter from contaminator.
. Used and disposed after
EACH use in order to prevent contamination and reduce clean up and sterilization
Autoclave
Removes microorganisms (virus, bacteria, fungi etc and spores using high pressure and high temperature steam sterilization.
USES:
• Sterilize contaminated material like biohazardous waste
• Sterilize glassware, plastics and pipettes for use of aseptic techniques.
• Sterilize liquids for use in experimentation.
Spectrometer/ Spectrophotometer
Produces light of desired wavelength and it passes through your sample and reaches the photmeter that measures its intensity.
• It reports the value as amount of light absorbed (Absorbance (ABS).
USES:
• Measure growth of microorganisms like bacteria and yeast or other cells grown in
suspension.
• Find concentration of proteins or nuclei acids.
• Detect contaminants
Centrifuge
Is an apparatus that rotates at high speed and separates substances of different densities.
USES:
• Remove cell elements from blood to provide cell free serum or plasma.
• Separate subcellular components e.g. mitochondria, nuclel etc.
• Remove Insoluble matter from extracted proteins.
• Pellet DNA, RNA, protein or lipids in isolation of macromolecules
Microcentrifuge tubes
Tapered, specially-engineered test tubes for centrifuge work, designed to resist the high G-forces induced by centrifugal forces.
Container that uses water to heat or maintain a constant temperature in a highly controlled manner.
USES:
• To heat or thaw fragile biological substances like mammalian cells.
• To maintain more homogenous temperature control when working with nucleic acids and proteins. ‘
Water Bath
stirs at the controlled speed to mix liquids
Uses:
Mix cells In suspensions
Mix reagents of an array to create homogenous solutions
Vortex
Used to mix or agitate solutions, often prior to placement in the centrifuge and/or spectrophotometer
.
USES:
• To incubate antibodies, chemical reactions during experimentations for mixing and reproducible results.
• To aerate media while growing bacterial or yeast cultures.
Shaker
Uses a light source at the base in combination with various lenses to magnify images tunicallv from 40x-1000x
Compound Light Microscope
Uses a light source in the top along with various lenses in the base to magnify objects from 40x to 1000x
Inverted Microscope
Sealed chamber with controls to manage temperature/ sometimes humidity and CO2 levels.
• Used to culture adherent cells under specific conditions mimicking normal or diseased in vivo environment.
Water Jacketed CO2 Incubator
