lab animal past q Flashcards
1
Q
This gland is not a specific pheromone producer gland
A
Rabbit’s face gland
2
Q
specific pheromone producer gland
A
Gerbil’s umbilical gland
Dog’s cicumanal gland
Hamster’s hip gland
3
Q
Which is not a method for genetic quality control of lab animals?
A
Back gross breeding
4
Q
methods for genetic quality control of lab animals?
A
Skin transplantation
SSLP (micro-satellite)
Biomedical markers
5
Q
Drawback of preference tests:
A
Animals choose in the base of short term conditions
6
Q
Which measurement is used to express energy requirement of laboratory animals?
A
Metabolic body weight
7
Q
Sentinels are
A
Animals used to evaluate a group’s health status
8
Q
Which method wouldn’t you use to get 1mL of blood from a rat?
A
Cardiac puncture
9
Q
You cannot buy this type of diet from a commercial supplier
A
Chemically defined diet
10
Q
you can buy this type of diet from a commercial supplier
A
commercial pellet
liquid diet
gel
11
Q
Not α2-agonist
A
Tolmetine
12
Q
α2-agonist
A
xilazine
detomidine
romofidine
13
Q
Not inherited disease of rat
A
Hypo-acidosis
14
Q
inherited disease of rat:
A
deaf
diabetes
obesity
15
Q
The basic method of rodent phenotyping is to look for
A
Chromosoma test
16
Q
parts of refinement of animal experimentation
A
Genetic homogeneity
Health status
Proper defined diet
17
Q
Which animals are covered by 63/2010 EEC directive
A
Vertebrates and Cephalipodes
18
Q
GLP not concerned about conditions under which laboratory
A
The ones being built
19
Q
Which species can die after oral penicillin treatment
A
Guinea pig
20
Q
One of them is an important mechanism of nutrigenomics
A
Changing the signalling pathway
21
Q
Acute toxicity test has an invasiveness category of __ pain
A
Severe
22
Q
Maintenance energy requirements of laboratory animals
A
0.45MJ DE x W0.75
23
Q
This disease is not a zoonosis
A
Ringtail
Sendai virus disease
24
Q
this disease is zoonosis
A
Lymphatic choriomeningitis of mice
Salmonellosis
Listeriosis
Hantaan virus disease
25
The recommended nutrient allowances
Ensure the reproduction and longevity of the animals
26
Cannot be stage of mouse genital and urinary syndrome
Ulceration
27
Can be stage of mouse genital and urinary syndrome
Bacteraemia
Inflammation
Mating overload
28
Quarantine period not determined by
Number of animals
29
Quarantine period determined by
health status of the animals
species
purpose of use
30
Which species has a tied skin, so cannot grab it without causing pain to the animal?
Guinea pig
31
Not physical euthanasia method
Maceration
32
physical euthanasia method
T61
Cervical dislocation
Decapitation
33
Which species is not a member of Rodentia genus
Marmoset
34
Which species is a member of Rodentia genus
Golden hamster
Gerbil
Guinea pig
35
OECD do not deal with questions about
Validation of in vitro methods
36
Gerbil is a good model for
Epilepsy aorta
37
animal cannot be examined with ultrasound
Golden hamster
38
Not true for chronic mycoplasmosis of mice
Zoonosis
39
true for chronic mycoplasmosis of mice
Agent is Mycoplasma pulmonis
12-18 day old puppies are most disposed
Can appear with Sendai virus
40
The closest species to Syrian hamster in taxonomy is
Gerbil
41
Good species to use for auditory research
Chinchilla
42
Breeding technique which is not used to maintain an out-bred stock
Brother-sister mating
43
Breeding technique which is used to maintain an out-bred stock:
Random breeding
MAI
cross-breeding
44
A contaminated breeding colony (line) should be
Euthanized
Put under negative pressure cabin
Treated by antibiotics
Individually marked
45
In reproductive toxicology, not acceptable treatment
Used only for parents before mating
Between implantation and foetogenesis
Between foetogenesis and parturition From parent before mating till separation from dam
46
It can be used for intra-dermal injection
Mice ear
47
Pasteurella pneumotropica does not attack
Brain
48
Pasteurella pneumotropica does attack
Uterus
Prostate
Lung
49
Environmental enrichment should not be done
Just for human fun
50
Environmental enrichment should be
For the benefit of the animals
With the sense of animals
With some unpredictability
51
How would you restrain a gerbil
Hand around the chest
52
Who will decide on institute level if an animal experiment was necessary or not
Ethical committee (Animal Welfare Body)
53
This female embryo in a rat will achieve breeding maturity (M=Month
1 M
54
The basic method of rodent sexing is to look fo
Ano-genital distance
55
The recommended nutrient allowances
Ensure the optimum functioning of the animals
56
Maintenance energy requirements of laboratory animals and for extrapolation of dosage use
W 0.75
57
The main body of Laboratory Animal Science of the World
CALAS (the Canadian association for laboratory animal science)
58
The closest species to Syrian hamster in taxonomy is
Gerbil
59
What ‘Environmental Enrichment’ means in laboratory
To give bedding material or toys to experimental animal if possible
60
Feeding rancid feed and/or vitamin E deficient feed may cause
Dermatitis
61
Zebradanio is
Omnivore
62
It is an alternative use of animals in experiments
Database search and meta-analysis
63
Sub-cutaneous injury has a severity category of __ pain
Minor
64
Which 3 words called the 3 R s by Russel and Burch
Reduction, Replacement, Refinement
65
It is not true about euthanasia
Can be used to get large amount of blood out of an animal
66
What does ‘knock out animal’ mean
Transgenic animal
67
Which method would you use to get a drop of blood from mice?
Retro-orbital puncture
68
Which name indicates an outbred stock?
.lr:NMRI
69
You cannot use this method for euthanasia on rat
Ketamine
70
You can use this method for euthanasia on rat
Decapitation
Cervical dislocation with weight limit
CO2 inhalation
71
What is the best equipment to maintain a transgenic colony
Base colony in different countries
72
Which species has only provoked (stimulated) ovulation
Ferret
73
What does the expression ‘inbred strain’ mean
Brother-sister mating through at least 20 generations
74
Which species is the best in atherosclerosis research
WHHL-rabbit
| Notes – Gerbil
75
You can find a ‘sacculus rotundus or ampulla ilei’ in the
Rabbit
76
This gland is a specific pheromone producer gland
Hamster’s lateral gland
77
Which species has a gallbladder
Golden hamster
78
What does SPF mean
Specific Pathogen Free
79
How would you restrain a rat for per-oral dosing
By grabbing the skin on back and neck
80
Which sentence is not true about ‘Environmental Enrichment’
Device in case that animal can control
81
true about ‘Environmental Enrichment’
All possibilities should be done to improve animal’s well-being what the experiment allows
Always should be evaluated on cross-benefit base
A nest material is an example for that
82
Gnotobiotic (=Axenic) animals
Germ free
83
What does rederivation mean?
Production of germ-free strain by embryo transfer
84
In the practice, it is not possible to produce germ-free colony from this species
Duck
85
Which would be the best feeding regime in an experiment
Ad libitum
86
What is well-being
State of physiological and psychological harmony of the animal with its internal and external environment
87
Which vein can be used for IV injection on ferret
Jugular vein
88
How would you restrain a rabbit
By grabbing as much skin as possible on back
89
You cannot use this method for euthanasia on new-born, nest-living animals
CO2 inhalation
90
Which method would you use you get 10mL of blood from an adult rat?
Preparation of abdominal aorta
91
Which mouse strain is sensitive to Pneumocystis carini
Swiss
92
Which is not necessary before transport of rodent?
Fasting
93
necessary before transport of rodent
Clinical examination
Ear printing
Acclimatisation
94
When this phenomenon disappears, the narcosis ends with death
Cornea reflex
95
It is not true about euthanasia
Should be cheap
96
true about euthanasia
Should achieve rapid unconciseness and death
Should be safe for user
Should be simple to administer
97
not true about the co-isogenic strains
It can only be maintained in a homozygous form
98
true about the co-isogenic strains
The new gene is the product of natural maturation
The name contains the strain/sub-strain and the symbol of the mutant gene
The co-isogenic strain only differs by only one gene from the original one
99
Dog is not a good model for
Cardiovascular atherosclerosis
100
Dog is a good model for
Tumour-cancer research
Leptospirosis
Epilepsy
101
Mini-pig has a live weight of
20-45 kg
102
Experimental design cannot be
Cross-breeding
103
Experimental design can be
Cross-over design
Latin square design
Factorial design
104
The basal circadian rhythm is
Dependent from the gen expression of a protein
105
Melatonin is produced by
Pineal gland
106
This species mostly used in viral respiratory research
Ferrets
107
A chronic stress can cause this type of behaviour
Apathy / Aggression
108
Well-being cannot be measured by (more than one correct answer)
Clinical assessment
Physiological parameters
Grimace score
Reproductive state
109
Which environment is good for animals?
Classical music
110
Which tissue is the least sensitive to suffering?
Brain tissue
111
The naked mole rat has an extra=ordinary long life-span because of
Special hyaluronan which prevent cancer
112
The co-efficient on inbreeding (F
Means the fraction of the genes that get fixed in a homozygous state
113
It is inherited disease of some mouse strains
Deafness
114
Which is a member of Pox viridae
Ectomelia virus
115
Ringtail is caused by
Too low relative humidity
116
Not part of refinement of animal experimentation
Hair colour
| Alternative use of animals
117
part of refinement of animal experimentation
Health status
Analgesia
Environmental enrichment
118
The hearing range of rodents
0.5 / 1 - 100 / 120 kHz
119
Which species has 1 pair teats
Guinea pig
120
Which species has a ‘peg’ (double incisors) teeth
Rabbit
121
The two important words for animals concerning the pain feeling (Weiss-trail)
Predictability and controllability
122
Mouse pox is
Caused by Ectomelia
123
It is not a physical euthanasia method
Cooling in refrigerator
124
a physical euthanasia method
T61
Cervical dislocation
Decapacitation
CO2 inhalation
125
The African clawed for (Xenopus)
Shows mating behaviour after Hcg injection
126
The axolotl is not
Reaches adulthood after a complete metamorphosis
127
The axolotl is
Capable of regenerating lost limbs completely
Carnivorous
Has a long lifespan (8-10 years)
128
Laboratory animals – except the guinea pig – usually adjust their feed intake to satisfy their
Energy requirement
129
Which species can die after oral antibiotic treatment
Guinea pig and rabbit
130
The role of PPAR is to
Burn fats
131
One of the following has no anti-cancer effect
Iron
132
the following has anti-cancer effect:
Resveratrol
CLA
Green tea
133
an important mechanism of epigenomics
Methylation of DNA
134
Zebrafish has a life-span of __year:
6-8
| 3-5 years according to internet
135
One of them has no role in epigenomics
Change of base in DNA
136
The role of __ is a fine regulation of the protein synthesis in the cell
miRNA / siRNA
137
In some cases the epigenetic changes may be inherited
If the effect(s) acts during the germ-cell proliferation (‘time window’)
138
You can find neoteny at
Axolotl
139
Pheromones are detected by
VNO
140
true about the ferret
We can prevent the constant estrus by using GnRH agonist implants
141
The cephalopods
Are under the protection of the 2010/63/EU Directive since 2013
142
One of the statements is true
Animals in an outbred stock are anisogenic
143
A chromorhnydachyrrhea
Vitamin A deficiency
144
It can go through the uteral wall
Mouse mammary tumour
| LCM
145
Carbohydrate ingestion
Stimulate the fatty acid synthase gene expression
146
May increase the mitochondria number
Resveratrol
147
The most used model for drug metabolism study for humans
Macaco
148
Describe the naked mole rat as an animal model.
- Have a gene which conserves collagen.
- Do not age.
- Do not get tumours.
- Life-span of 20-30 years.
- Complex social system and hierarchy.
- Best model for aging and cancer studies
149
Describe the evaluation of the Glires pain categories
- Based on appearance, behaviour, pulse, appetite/drinking, cardiovascular, digestive, and nervous systems.
- 0-4 = Normal
- 5-9 = Needs analgesia
- 10-14 = Moderate pain, possible need to terminate trial
- 15-20 = Not physiological, stop experiment!
150
Explain the pain scoring system for Glires
- 0 points = None
- 1 point = Mild
- 2 points = Moderate
- 3 points = Severe
151
Explain the epigenetics’ mechanism of action
- Modifications e.g. Methylation, histone acetylation, which protect DNA and modify which genes are expressed or silenced.
- Do not change the genome itself
- May be inherited
- Influenced by outside stimuli e.g. nutritional status
