(lab) amylase Flashcards
major sources of amylase
pancreas
salivary gland
minor sources of amylase
skeletal muscle
liver
fallopian tube
amylase catalyzes breakdown of __ and __ with products __, __, __
starch, glycogen; glucose, maltose, dextrin
amylase requires __ and __ for activation
calcium and chloride ions
disease where AMS combines with Ig to form a large complex that is not filterable by the glomerulus
macroamylasemia
isoenzyme: predominates in urine
P-type
p-type isoenzyme markedly elevated in acute pancreatitis
P3
isoenzyme: predominates in serum
S-type
t/f: S-type isoenzyme migrates slower than p-type
f (faster)
S-type AMS is inhibited by __
wheat germ lectin
to measure s-ams and p-ams, determine the __ in the presence and absence of __
t-ams, lectin
amylase methods: measure the disappearance of starch substrate
amyloclastic
amylase methods: measures the appearance of the product
saccharogenic
amylase methods: measures the increasing color from production of product couples with chromogenic dye
chromogenic
amylase methods: coupling of several enzyme systems to monitor amylase activity
coupled enzyme / continuous monitoring
color of starch+iodine
purplish/dark blue
t/f: product of amyloclastic/ iodometric method is colorless
t
t/f: in amyloclastic / iodometric method, fast disappearance of starch substrate means low AMS activity
f (high AMS activity)
in saccharogenic method, amount of reducing sugar AMS activity is reported in __ units
somogyi
in chromolytic / chromogen labelled substrate method, the higher the color intensity of the soluble dye-substrate, the __(higher, lower) the AMS activity
higher
in coupled enzyme reaction / continuous monitoring method, change in absorbance of __ at __nm and __pH is measured
absorbance of NAD+, 340nm, 6.9 pH
enzymes used in coupled enzyme reaction
ams
a-glucosidase
hexokinase
g-6-pd
t/f: morphine and other opiates for pain relief may falsely decrease AMS
f (falsely increase)
t/f: oxalates and citrates may falsely decrease AMS
t
reference range
serum: 28-100 u/L
urine: 1-15 U/hour
substrate used in amylase testing
2-chloro-4-nitrophenyl-maltotrioside
buffer used
MES (ph 6.0)
wavelength
405nm
substrate used in coupled enzymatic method
maltotetrase / maltopentoase
