LAB A: 1.2 Micropipette techniques

Question 1

function and volume range of a micropipette

Answer 1

to measure small volume of liquid
0.5 - 1000 uL

Question 2

Plunger

Answer 2

top most part
adjusts the volume and aspirate

Question 3

rotation of the plunger for increase/decrease

Answer 3

anticlockwise - increase
clockwise- decrease

Question 4

tip ejector

Answer 4

used to remove the micropipette tips

Question 5

volume window

Answer 5

see the amount of quantity

Question 6

shaft

Answer 6

tube filled with air which sucks and release the volume

Question 7

pipette tip

Answer 7

attached at the end; collect and transfer the sample

Question 8

P-20

Answer 8

2-20 uL white

Question 9

p-200

Answer 9

20-200 uL yellow

Question 10

p-1000

Answer 10

100-1000uL blue

Question 11

type of micropipette base on working principle

Answer 11

air displacement micropipette- the volume of the air displaced corresponds to the same amount of aspiration volume

positive displacement pipette- the piston moves up and down with no air included

Question 12

types of micropipette based on working mechanism

Answer 12

mechanical pipette- operated by hand
electronic micropipette- automatic operation

Question 13

types of micropipette based on the no. of channels

Answer 13

single channel micropipette-
multi-channel micropipette- 8, 12, 16

Question 14

types of micropipette based on volume capacity

Answer 14

fixed volume
adjustable volume- the amoun of liquid to be aspirated can be changed