LAB 6 Flashcards
DNA Electrophoresis:
Running DNA on an agarose on an agarose gel using an electrical current to separate the bands or fragments.
A band or fragment is composed of ______:
Many pieces of DNA are of the same length.
A DNA control:
It is always electrophoresed with the other DNA.
This allows you to determine the size of the band in the DNA of interest.
How many pieces of DNA will be in each band:
The same in each band irrespective of size.
Band in the same lane contains:
The same sequence of nucleotides.
Why aren’t the thickness of the bands in lane 2 proportional to those in lane 1:
Difference lanes don’t have the same concentration of DNA.
In a digest of a sample of DNA, the bands within that sample should be:
Proportional in terms of thickness and intensity versus size.
Bands of two different samples would be proportional in terms of:
The band size and band migration down the gel.
Why is DNA gel electrophoresis done:
It allows you to separate bands and determine their sizes.
You could use it to make sure the plasmid has not been altered.
What does the gel contain that allows it to conduct electricity:
A buffer.
DNA samples are loaded:
In the wells.
What stains the gel:
Ethidium bromide (it is a mutagen).
It gets between the bases (intercalates).
It has a ring structure (4 rings).
what Involves pouring melted agarose into a gel tray and a comb is used to make the well:
Pouring gel.
The buffer solution is also called:
Peacock solution.
Purpose of the loading dye:
It weights down the DNA so it doesn’t flow out.
It creates a dye front so you can figure out how far the DNA has run on the gel.
