Lab 6

Question 1

differential stain

Answer 1

provides more than just size, shape, and arrangement, structural differences
- gram positive - high peptidoglycan low lipid
- gram negative - low peptidoglycan high lipid

Question 2

how nutrients and temperature can directly influence bacterial growth? ***

Answer 2

cardinal temperature points
min, max and optimal growth temp.

Question 3

Minimum, maximum and optimal growth temperature

Answer 3

min - lowest temp where growth occurs
max - highest temp where growth occurs
optimum - its where they are found growing (natural environment)

Question 4

what does psychrophiles grow at?

Answer 4

micrbes that grow within a temp ranfe of less than 0 degrees C and 20 C

Question 5

mesophiles

Answer 5

minimum - 15-30 C
max - above 45 C
optimal - 30-40 C
(our bodys are 37)
- main cause of infections

Question 6

thermophiles

Answer 6

min - 45 C
max - exceed 100C
optimal - 50 -85 C

Question 7

Thermophiles are archaea but are archaea classified as hyperthermophiles

Answer 7

Yes because it exceeds 80 degrees C

Question 8

what is the difference of an enriched media, chemically defined media, EZ medium, complex

Answer 8

• enriched - contains growth factors, vitamins, and other essential nutrients to promote growth of fastidious organisms
• chemically defined - chemical composition of medium is known
• EZ - chemical components are exactly known
• Complex media - which contains extractions and digests of yeast, meat or plants and percise chemical composition not known.