Lab 4 Enzyme Activity Flashcards

1
Q

Review micropipette

A

Ok

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2
Q

What is the nature of a spontaneous process and what is the distribution of energy?

A

It is exergonic

Energy is released usually in the form of heat

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3
Q

What is the second law of thermodynamics?

A

Entropy increases

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4
Q

What is the first law of thermodynamics?

A

Energy can neither be created or destroyed

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5
Q

How to enzymes speed up reactions ?

A

They reduce the amount of activation energy required to complete the reaction

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6
Q

Why are activation energy barriers Important ?

A

Aids in the ability store macromolecules

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7
Q

How can activation energy barriers be overcome?

A

Addition of an enzyme or increasing temperature

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8
Q

What is the role of an enzyme?

A

To speed up chemical reactions

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9
Q

How do enzymes enable life?

A

Allows for reactions to occur within a biological time scale

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10
Q

What are enzymes made from?

A

Proteins

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11
Q

What is an enzyme substrate?

A

A reactant that requires the aid of an enzyme to produce products

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12
Q

How do enzymes recognize and bind to their substances? Shape important?

A

Specific amino acids make up the activation site which only match certain substrates.
Shape is important

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13
Q

Does heating up an enzyme denature it?

A

Yes

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14
Q

Why do denatured proteins produce cloudy substances when heated?

A

Denatured proteins clump together

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15
Q

Why does increasing temperature increase the rate of chemical reactions?

A

It increases the chance of chemical collisions

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16
Q

What is Lugols solution used for?

A

Indicated the presence of starch

17
Q

What is Benedict’s solution used for?

A

Indicator for sugars

18
Q

What does litmus indicate?

A

The pH of a solution

19
Q

How is a synthetic substrate different from an indicator?

A

Synthetic substrate binds to the active site and is cleaved

Indicator binds to the substrate

20
Q

Can RNA act as an enzyme?

A

Yes