Lab 4 Enzyme Activity Flashcards
Review micropipette
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What is the nature of a spontaneous process and what is the distribution of energy?
It is exergonic
Energy is released usually in the form of heat
What is the second law of thermodynamics?
Entropy increases
What is the first law of thermodynamics?
Energy can neither be created or destroyed
How to enzymes speed up reactions ?
They reduce the amount of activation energy required to complete the reaction
Why are activation energy barriers Important ?
Aids in the ability store macromolecules
How can activation energy barriers be overcome?
Addition of an enzyme or increasing temperature
What is the role of an enzyme?
To speed up chemical reactions
How do enzymes enable life?
Allows for reactions to occur within a biological time scale
What are enzymes made from?
Proteins
What is an enzyme substrate?
A reactant that requires the aid of an enzyme to produce products
How do enzymes recognize and bind to their substances? Shape important?
Specific amino acids make up the activation site which only match certain substrates.
Shape is important
Does heating up an enzyme denature it?
Yes
Why do denatured proteins produce cloudy substances when heated?
Denatured proteins clump together
Why does increasing temperature increase the rate of chemical reactions?
It increases the chance of chemical collisions