Lab 3 - Bacteriophage and little eukaryotes

Question 1

bacteriophage

Answer 1

obligate intracellular parasites

• need a metabolically active bacterial host to replicate

a virus that infects and replicates within bacteria and archaea.

• prob all proks are susceptible

Question 2

phage contents

Answer 2

protein shell or capsid that surrounds and contains the viral nucleic acid

Question 3

lytic cycle

Answer 3

redirection of all bacterial metabolic processes

ie synthesis of only phage nucleic acid and protein occurs - hundreds of new phage particles assemble in the bacterial cytoplasm

Question 4

virulent phage

Answer 4

cause cell to lysis

Question 5

temperate phage

Answer 5

do not cause lysis initially, but establish a lysogenic relationship

phage dna coexists in host cell

Question 6

incubating a bacterial culture with an infective phage

Answer 6

demonstrates bacterial host cell lysis.

evidence of infection = reduction/ loss of turbidity in a culture or in the form of zones of clearing on an agar plate

Question 7

t4 phage

Answer 7

infect e. coli

Question 8

countable plate

Answer 8

30-300 plaques (groups)

Question 9

phage particles per mL or plaque forming units /mL

Answer 9

PFU/ mL

plaques observed / dilution of sample x what makes it over 1mL

Question 10

contact made with phage

Answer 10

evident clear zone from host cell lysis

Question 11

prokaryotes

Answer 11

largest and most direct effect on our immediate environment and on our bodies

Question 12

cyanobacteria

Answer 12

large diverse group of prokaryotes common in natural environment

Question 13

protozoa

Answer 13

single celled eukaryotes

Question 14

algae

Answer 14

euks
distinguished by chlorophyll

are oxygenic phototrophs
have cell walls often w flagella

Question 15

detrius feeders

Answer 15

ingest only organic debris or dead cells