Lab 3 and 4 Flashcards

1
Q

Cell lysis

A

breaking of the cell

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2
Q

Lysozyme

A

enzyme that breaks down peptidoglycans of the cell wall

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3
Q

Peptidoglycan

A

A polymer consising of sugars and amino acids

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4
Q

What are some methods of cell lysis?

A
  • sonification (uses ultrasound waves )
  • liquid homogenization ( uses hydraulic pressure )
  • freeze- thaw method ( freeze in dry ice or ethanol and then thaw at room temp. )
  • bead beating ( agitates sample by grinding with beads or media)
  • microfluidizer method ( high pressure homogenizer)
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