Lab 3 and 4 Flashcards
1
Q
Cell lysis
A
breaking of the cell
2
Q
Lysozyme
A
enzyme that breaks down peptidoglycans of the cell wall
3
Q
Peptidoglycan
A
A polymer consising of sugars and amino acids
4
Q
What are some methods of cell lysis?
A
- sonification (uses ultrasound waves )
- liquid homogenization ( uses hydraulic pressure )
- freeze- thaw method ( freeze in dry ice or ethanol and then thaw at room temp. )
- bead beating ( agitates sample by grinding with beads or media)
- microfluidizer method ( high pressure homogenizer)