Lab 3

Question 1

Define the terms oxygenation, deoxygenation, ferrous, and ferric in relation to haemoglobin?

Answer 1

Oxygenation:
The process of oxygen binding to the iron in hemoglobin, converting deoxyhemoglobin (Hb) into oxyhemoglobin (HbO₂).

Deoxygenation:
The release of oxygen from hemoglobin, converting oxyhemoglobin (HbO₂) back to deoxyhemoglobin (Hb).

Ferrous (Fe²⁺):
The +2 oxidation state of iron in hemoglobin, which allows it to bind oxygen.

Ferric (Fe³⁺):
The +3 oxidation state of iron in hemoglobin, seen in methemoglobin, which cannot bind oxygen effectively.

Question 2

What is it called where absorbance is equal between two different forms of a molecule?

Answer 2

The isosbestic point

Question 3

Describe the tertiary structure of a G Protein-Coupled Receptor (GPCR).

Answer 3

The receptor protein folds to form a bundle of seven transmembrane a-helices, connected by three intracellular and three extracellular loops. The N-terminus of the receptor protein is Inside Cell outside the cell, whilst the C-terminus of the protein is inside the cell